Results 41 to 50 of about 2,028,343 (313)

7 Glycogen and Starch Debranching Enzymes

, 1971
This chapter focuses on glycogen and starch debranching enzymes. The debranching enzymes can be divided into two classes—namely, direct debranching enzyme and indirect debranching enzymes.
W.J. Whelan, Lee, E.Y.C, E.Y.C. Lee, Whelan, W.J   +3 more
core   +1 more source

Quantitative comparison of catalytic mechanisms and overall reactions in convergently evolved enzymes : implications for classification of enzyme function [PDF]

, 2010
The authors thank the National Institutes of Health (NIH R01 GM60595 to PCB) and the Scottish Universities Life Sciences Alliance (SULSA to JBOM) for funding.Functionally analogous enzymes are those that catalyze similar reactions on similar substrates ...
Almonacid, Daniel E, Daniel E Almonacid, Yera, Emmanuel R., Yera, Emmanuel R, Babbitt, Patricia C., Babbitt, Patricia C, John B O Mitchell, Almonacid Daniel E., Almonacid, Daniel E., Patricia C Babbitt, Yera Emmanuel R., Mitchell, John BO, Mitchell John B. O., Babbitt Patricia C., Emmanuel R Yera, Mitchell, John B. O.   +15 more
core   +2 more sources

High-temperature ssBP-LAMP breaks the barrier of nonspecific amplification and unlocks the full potential of LAMP diagnostics

Cellular & Molecular Biology Letters
Background Loop-mediated isothermal amplification (LAMP) is a highly attractive nucleic acid detection method for decentralized molecular diagnostics, but its broader adoption has been limited by unpredictable nonspecific amplification (NSA).
Rita S. Simões, Pedro Bule, Carlos M. G. A. Fontes, João S. Teodoro   +3 more
doaj   +1 more source

High copy number and highly stable Escherichia coli–Bacillus subtilis shuttle plasmids based on pWB980

Microbial Cell Factories, 2020
Background pWB980 derived from pUB110 is a promising expression vector in Bacillus for its high copy number and high stability. However, the low transformation rate of recombinant plasmids to the wild cells limited the application of it.
XingYa Zhao, JianYong Xu, Ming Tan, Jie Zhen, WenJu Shu, ShiBin Yang, YanHe Ma, HongChen Zheng, Hui Song   +8 more
doaj   +1 more source

Determination of Volatile Organic Compounds and Antibacterial Activity of the Amazonian Cyanobacterium Synechococcus sp. Strain GFB01

Molecules, 2020
Cyanobacteria exhibit great biotechnological potential due to their capacity to produce compounds with various applicability. Volatile organic compounds (VOCs) possess low molecular weight and high vapor pressure.
Samuel Cavalcante do Amaral, Agenor Valadares Santos, Maria Paula da Cruz Schneider, Joyce Kelly Rosário da Silva, Luciana Pereira Xavier   +4 more
doaj   +1 more source

Glycosyltransferase engineering and multi-glycosylation routes development facilitating synthesis of high-intensity sweetener mogrosides

iScience, 2022
Summary: Mogrosides are widely served as natural zero-calorie sweeteners. To date, the biosynthesis of high-intensity sweetness mogrosides V from mogrol has not been achieved because of inefficient and uncontrollable multi-glycosylation process.
Jiao Li, Shicheng Mu, Jiangang Yang, Cui Liu, Yanfei Zhang, Peng Chen, Yan Zeng, Yueming Zhu, Yuanxia Sun   +8 more
doaj   +1 more source

Reciprocal control of viral infection and phosphoinositide dynamics

FEBS Letters, EarlyView.
Phosphoinositides, although scarce, regulate key cellular processes, including membrane dynamics and signaling. Viruses exploit these lipids to support their entry, replication, assembly, and egress. The central role of phosphoinositides in infection highlights phosphoinositide metabolism as a promising antiviral target.
Marie Déborah Bancilhon, Bruno Mesmin
wiley   +1 more source

B12-dependent radical SAM enzymes: Ever expanding structural and mechanistic diversity

, 2023
International audienceIn the last decade, B12-dependent radical SAM enzymes have emerged as central biocatalysts in the biosynthesis of a myriad of natural products.
Benjdia, Alhosna, Berteau, Olivier
core   +1 more source

Phosphatidylinositol 4‐kinase as a target of pathogens—friend or foe?

FEBS Letters, EarlyView.
This graphical summary illustrates the roles of phosphatidylinositol 4‐kinases (PI4Ks). PI4Ks regulate key cellular processes and can be hijacked by pathogens, such as viruses, bacteria and parasites, to support their intracellular replication. Their dual role as essential host enzymes and pathogen cofactors makes them promising drug targets.
Ana C. Mendes, Guilherme M. Azevedo, Amanda P. Barcellos, Diana Bahia   +3 more
wiley   +1 more source

The equilibrium model for the effect of temperature on enzymes: Insights and implications [PDF]

, 2008
A new, experimentally-validated “Equilibrium Model” describes the effect of temperature on enzymes, and provides a new mechanism for the reversible loss of enzyme activity with temperature.
Lee, Charles Kai-Wu, Weinberg, Cristina S., Monk, Colin R., Daniel, Roy M., Danson, Michael J.   +4 more
core