Results 51 to 60 of about 2,144,314 (302)

Biophysical characterization of the structural stability of Helix lucorum hemocyanin

Biotechnology & Biotechnological Equipment, 2021
The structural stability of the hеmocyanin purified from the hеmolymph of gаrden snаils Helix lucorum (HlH) was investigated by means of far-UV circular dichroism (CD), differential scanning calorimetry (DSC) and transmission electron microscopy (TEM ...
Krassimira Idakieva, Svetla Todinova, Aleksandar Dolashki, Lyudmila Velkova, Yuliana Raynova, Pavlina Dolashka   +5 more
doaj   +1 more source

Glycosyltransferase engineering and multi-glycosylation routes development facilitating synthesis of high-intensity sweetener mogrosides

iScience, 2022
Summary: Mogrosides are widely served as natural zero-calorie sweeteners. To date, the biosynthesis of high-intensity sweetness mogrosides V from mogrol has not been achieved because of inefficient and uncontrollable multi-glycosylation process.
Jiao Li, Shicheng Mu, Jiangang Yang, Cui Liu, Yanfei Zhang, Peng Chen, Yan Zeng, Yueming Zhu, Yuanxia Sun   +8 more
doaj   +1 more source

Effect of DFPP on Ferroptosis in Peripheral Blood CD14+ Monocytes of Hyperlipidemic Patients Involving the ACSL4/LPCAT3/ALOX5 Pathway

Therapeutic Apheresis and Dialysis, EarlyView.
ABSTRACT Introduction Hyperlipidemia is a major risk factor for atherosclerotic cardiovascular disease (ASCVD). Although statins are the cornerstone of lipid‐lowering therapy, their use may be limited in some patients by adverse effects. This study evaluated the possible impact of non‐drug treatment using double‐filtration plasmapheresis (DFPP) on CD14+
Yun Cheng, Lan Li, Qingmei Yang, Meipeng Wu   +3 more
wiley   +1 more source

7 Glycogen and Starch Debranching Enzymes

, 1971
This chapter focuses on glycogen and starch debranching enzymes. The debranching enzymes can be divided into two classes—namely, direct debranching enzyme and indirect debranching enzymes.
W.J. Whelan, Lee, E.Y.C, E.Y.C. Lee, Whelan, W.J   +3 more
core   +1 more source

High-temperature ssBP-LAMP breaks the barrier of nonspecific amplification and unlocks the full potential of LAMP diagnostics

Cellular & Molecular Biology Letters
Background Loop-mediated isothermal amplification (LAMP) is a highly attractive nucleic acid detection method for decentralized molecular diagnostics, but its broader adoption has been limited by unpredictable nonspecific amplification (NSA).
Rita S. Simões, Pedro Bule, Carlos M. G. A. Fontes, João S. Teodoro   +3 more
doaj   +1 more source

High copy number and highly stable Escherichia coli–Bacillus subtilis shuttle plasmids based on pWB980

Microbial Cell Factories, 2020
Background pWB980 derived from pUB110 is a promising expression vector in Bacillus for its high copy number and high stability. However, the low transformation rate of recombinant plasmids to the wild cells limited the application of it.
XingYa Zhao, JianYong Xu, Ming Tan, Jie Zhen, WenJu Shu, ShiBin Yang, YanHe Ma, HongChen Zheng, Hui Song   +8 more
doaj   +1 more source

Enteropathogenic E. coli shows delayed attachment and host response in human jejunum organoid‐derived monolayers compared to HeLa cells

FEBS Letters, EarlyView.
Enteropathogenic E. coli (EPEC) infects the human intestinal epithelium, resulting in severe illness and diarrhoea. In this study, we compared the infection of cancer‐derived cell lines with human organoid‐derived models of the small intestine. We observed a delayed in attachment, inflammation and cell death on primary cells, indicating that host ...
Mastura Neyazi, Pilar Samperio Ventayol, Natalie Burkard, Nicolas Schlegel, Carmen Aguilar, Sina Bartfeld   +5 more
wiley   +1 more source

Determination of Volatile Organic Compounds and Antibacterial Activity of the Amazonian Cyanobacterium Synechococcus sp. Strain GFB01

Molecules, 2020
Cyanobacteria exhibit great biotechnological potential due to their capacity to produce compounds with various applicability. Volatile organic compounds (VOCs) possess low molecular weight and high vapor pressure.
Samuel Cavalcante do Amaral, Agenor Valadares Santos, Maria Paula da Cruz Schneider, Joyce Kelly Rosário da Silva, Luciana Pereira Xavier   +4 more
doaj   +1 more source

Gut microbiome and aging—A dynamic interplay of microbes, metabolites, and the immune system

FEBS Letters, EarlyView.
Age‐dependent shifts in microbial communities engender shifts in microbial metabolite profiles. These in turn drive shifts in barrier surface permeability of the gut and brain and induce immune activation. When paired with preexisting age‐related chronic inflammation this increases the risk of neuroinflammation and neurodegenerative diseases.
Aaron Mehl, Eran Blacher
wiley   +1 more source

The equilibrium model for the effect of temperature on enzymes: Insights and implications [PDF]

, 2008
A new, experimentally-validated “Equilibrium Model” describes the effect of temperature on enzymes, and provides a new mechanism for the reversible loss of enzyme activity with temperature.
Lee, Charles Kai-Wu, Weinberg, Cristina S., Monk, Colin R., Daniel, Roy M., Danson, Michael J.   +4 more
core