Results 151 to 160 of about 396,299 (201)

In silico mutation of aromatic with aliphatic amino acid residues in Clostridium perfringens epsilon toxin (ETX) reduces its binding efficiency to Caprine Myelin and lymphocyte (MAL) protein receptors

Journal of Biomolecular Structure and Dynamics, 2023
Enterotoxaemia (ET) is a severe disease that affects domestic ruminants, including sheep and goats, and is caused by Clostridium perfringens type B and D strains.
Sunil Kumar, S. Behera, K. Gururaj, Anurag Chaurasia, Sneha Murmu, R. Prabha, U. Angadi, R. Pawaiya, A. Rai   +8 more
semanticscholar   +1 more source

Carboxyl groups in Clostridium perfringens epsilon toxin

Microbial Pathogenesis, 1987
The maximal number of norleucine methyl ester (NME) incorporated into carboxyl groups in epsilon toxin of Clostridium perfringens by the carbodiimide-nucleophile procedure was 7 and 17 in the absence and presence of 8 M urea, respectively. The introduction of 3-4 nucleophilic modifying agents such as NME, glycine methyl ester or taurine into carboxyl ...
J, Sakurai, M, Nagahama
openaire   +2 more sources

Enterocolitis in goats associated with enterotoxaemia in the perspective of two toxins: Epsilon toxin and beta-2 toxin - An immunohistochemical and molecular study.

Comparative Immunology, Microbiology & Infectious Diseases, 2022
Caprine intestinal diseases associated with clostridia are generally caused by Cpa and Etx encoded alpha (α) and epsilon (ε) toxinotypes of Clostridium perfringens type D respectively. A recent study on goat enterocolitis, demonstrated that the incidence
N. Gangwar, R. Pawaiya, K. Gururaj, D. Andani, A. Kumar, R. Singh, A.P. Singh   +6 more
semanticscholar   +1 more source

Detection of Clostridium perfringens epsilon toxin by elisa

Research in Veterinary Science, 1987
An enzyme-linked immunosorbent assay (ELISA) has been developed as an alternative to neutralisation tests in mice to detect Clostridium perfringens type D epsilon toxin in the intestinal contents of animals which have died from suspected enterotoxaemia. The test was sensitive and quantitative and gave excellent agreement with the mouse protection test.
R D, Naylor, P K, Martin, R T, Sharpe
openaire   +2 more sources

Cytotoxic effects of epsilon toxin from Clostridium perfringens on colon cancer cell lines (HT-29) and (Caco2) in terms of oxidative stress and inflammatory cytokines.

Toxicological Research
Bacterial toxins, such as Epsilon toxin (Etx) from Clostridium perfringens, represent a promising, albeit underexplored, avenue for anticancer agent development.
SeyedAmir Mirabbasi, M. Ghane
semanticscholar   +1 more source

Production and purification of Clostridium perfringens type D epsilon toxin and IgY antitoxin.

Anaerobe, 2021
The aim of this study was to purify Clostridium perfringens type D epsilon toxin and produce and purify anti-epsilon chicken immunoglobulin Y (IgY).
G. G. Alves, L. A. Gonçalves, R. A. Assis, C. O. Oliveira Júnior, R. Silva, L. Heneine, F. Lobato   +6 more
semanticscholar   +1 more source

Attenuated Clostridium perfringens Epsilon Toxin Mutants and Insights Into the Mechanism of Attenuation

Journal of Basic Microbiology
Clostridium perfringens epsilon toxin (ETX) is a potent pore‐forming exotoxin responsible for severe enterotoxemia and necrotizing enterocolitis in ruminants.
Zibei Huang, Haiyan Wang, Jinlin Huang, Wenbo Liu   +3 more
semanticscholar   +1 more source

Cellular vacuolation induced by Clostridium perfringens epsilon‐toxin

The FEBS Journal, 2011
The epsilon‐toxin of Clostridium perfringens forms a heptamer in the membranes of Madin–Darby canine kidney cells, leading to cell death. Here, we report that it caused the vacuolation of Madin–Darby canine kidney cells. The toxin induced vacuolation in a dose‐dependent and time‐dependent manner. The monomer of the toxin formed oligomers on lipid rafts
Masahiro, Nagahama, Yukari, Itohayashi, Hideki, Hara, Masahiro, Higashihara, Yusuke, Fukatani, Teruhisa, Takagishi, Masataka, Oda, Keiko, Kobayashi, Ichiro, Nakagawa, Jun, Sakurai   +9 more
openaire   +2 more sources

Novel competitive annealing-mediated isothermal amplification (CAMP)-based detection of epsilon toxin gene in Clostridium perfringens.

Anaerobe
In this study, we developed a novel competitive annealing-mediated isothermal amplification (CAMP)-based assay for the detection of the etx gene, which encodes the epsilon toxin, of Clostridium perfringens.
A. Milton, Torik Basar, K. Srinivas, A. G. Momin, Sabia Khan, G. B. Priya, Samir Das, S. Ghatak, K. Puro   +8 more
semanticscholar   +1 more source

Identification of Effective Inhibitors against Epsilon Toxin (ETX) of Clostridium perfringens: Virtual Screening and Molecular Dynamics Simulation.

Toxicon
In spite of the potential for toxicity in humans, no therapeutics are approved against Epsilon toxin (ETX) for human use. Therefore, finding effective inhibitors for this toxin has garnered a lot of attention.
Atefe Panahipoor Javaherdehi, Z. Hashemi, Sepideh Khaleghi, Gholamreza Bayazian   +3 more
semanticscholar   +1 more source