Results 71 to 80 of about 15,432 (253)

Synergistic perspectives—How single‐molecule biophysics complement biochemical understanding

open access: yesFEBS Letters, EarlyView.
In this review, we discuss how ensemble biochemistry and single‐molecule approaches are complementary, outline commonly used single‐molecule techniques, and illustrate their relevance through two representative case studies: chromatin organization by SMC complexes and pathway choice during DNA double‐strand break repair.
Sara De Bragança   +2 more
wiley   +1 more source

Functional comparison of EncB and EncC cargo proteins in iron storage within the Myxococcus xanthus encapsulin

open access: yesFEBS Letters, EarlyView.
Encapsulins are protein nanocompartments that play an important role in iron storage. In the Myxococcus xanthus encapsulin system, two cargo proteins called EncB and EncC contribute to iron mineralization. Here, we show that EncB and EncC generate iron‐containing minerals with distinct chemical compositions, suggesting that the composition of stored ...
Harry B. McDowell   +2 more
wiley   +1 more source

Structural and biochemical analysis of a B12 superbinder

open access: yesFEBS Letters, EarlyView.
BtuG proteins are vitamin B12 scavengers in Bacteroides thetaiotaomicron, a dominant human gut bacterium. We present crystal structures of three BtuG homologs bound to cobalamin and its precursor cobinamide, revealing picomolar binding affinities, among the highest known for any natural protein.
Jose M. Martinez Felices   +3 more
wiley   +1 more source

Standard errors of Levine linear equating

open access: yes, 1993
The delta method was used to derive standard errors (SEs) of the Levine observed score and Levine true score linear equating methods. SEs with a normality assumption as well as without a normality assumption were derived.
Zeng, Lingjia   +2 more
core   +1 more source

Generalized kernel equating with applications in R

open access: yes
Generalized Kernel Equating is a comprehensive guide for statisticians, psychometricians, and educational researchers aiming to master test score equating.
González Burgos, Jorge Andrés   +5 more
core   +2 more sources

Is pseudo-equivalent groups equating via minimum discriminant information adjustment effective? Evidence from simulation and real data

open access: yesFrontiers in Education
The traditional way to linking different forms of test typically uses common (or randomly equivalent) examinees or items. When common examinees or items are unavailable due to test properties or administrative restrictions, equating with pseudo ...
Xizhen Fan   +4 more
doaj   +1 more source

PERBANDINGAN METODE PENYETARAAN SKOR TES MENGGUNAKAN BUTIR BERSAMA DAN TANPA BUTIR BERSAMA

open access: yesJurnal Kependidikan: Penelitian Inovasi Pembelajaran, 2016
Abstrak   Penelitian ini bertujuan untuk mengetahui kesetaraan skor 20 perangkat tes ujian akhir SMP dan membandingkan penyetaraan dengan butir bersama (equating) dan tanpa butir bersama (concordance).
Heri Retnawati
doaj   +1 more source

From junk to function — How weak selection in eukaryotes builds new parts and drives genomic complexity

open access: yesFEBS Letters, EarlyView.
How do genomes gain new functional parts? In eukaryotes, which tend to evolve under weak selection, much of the genome is junk. Palazzo and Qiu borrow the logic of Markov chains to show how non‐functional DNA becomes functional through the appearance of intermediate states, which arise due to epistasis, buffering, and biochemical messiness, allowing ...
Alexander F. Palazzo, Yi Qiu
wiley   +1 more source

The Shewanella oneidensis Fic enzyme SoFic targets the switch‐I region of EF‐Tu for AMPylation

open access: yesFEBS Letters, EarlyView.
Fic enzymes mediate diverse post‐translational modifications across all domains of life, including AMPylation. Prokaryotic EF‐Tu can be AMPylated and deAMPylated by the conserved Fic enzyme SoFic. Structural and biochemical approaches were used to characterize the effect of AMPylation on EF‐Tu, SoFic's enzymatic activities, and the enzyme‐target ...
Svenja Runge   +6 more
wiley   +1 more source

Chiral separation of chloroalkanes with the chromatographic column onboard Martian rovers

open access: yesFEBS Letters, EarlyView.
Computer image of the Rosalind Franklin Rover of ESA's ExoMars mission. ExoMars is scheduled to land on planet Mars in Oxia Planum in 2029. This area represents an interesting spot to look for biosignatures. Investigations of ExoMars include measurement on molecular chirality. We show that chiral chloroalkanes, that have been identified on Mars, can be
Asma Merzougui   +4 more
wiley   +1 more source

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