Results 101 to 110 of about 2,369,262 (399)

EcoCyc: fusing model organism databases with systems biology. [PDF]

, 2012
EcoCyc (http://EcoCyc.org) is a model organism database built on the genome sequence of Escherichia coli K-12 MG1655. Expert manual curation of the functions of individual E.
Bonavides-Martínez, César, Collado-Vides, Julio, Fulcher, Carol, Gama-Castro, Socorro, Gunsalus, Robert P, Huerta, Araceli M, Karp, Peter D, Keseler, Ingrid M, Kothari, Anamika, Krummenacker, Markus, Latendresse, Mario, Mackie, Amanda, Muñiz-Rascado, Luis, Ong, Quang, Paley, Suzanne, Paulsen, Ian, Peralta-Gil, Martin, Santos-Zavaleta, Alberto, Schröder, Imke, Shearer, Alexander G, Subhraveti, Pallavi, Travers, Mike, Weerasinghe, Deepika, Weiss, Verena   +23 more
core   +1 more source

A general model for analysis of linear and hyperbolic enzyme inhibition mechanisms

FEBS Open Bio, EarlyView.
We developed a general enzyme kinetic model that integrates these six basic inhibition mechanism onto a single one. From this model, we deduced a general enzyme kinetic equation that through modulation of simple parameters, γ (the relative inhibitor affinity for two binding sites) and β (the reactivity of the enzyme–substrate–inhibitor complex), is ...
Rafael S. Chagas, Sandro R. Marana
wiley   +1 more source

Source Attribution Study of Sporadic Salmonella Derby Cases in France

Frontiers in Microbiology, 2020
Salmonella enterica subsp. enterica serovar Derby is one of the most frequent causes of gastroenteritis in humans. In Europe, this pathogen is one of the top five most commonly reported serovars in human cases. In France, S.
Yann Sévellec, Sophie A. Granier, Sophie A. Granier, Simon Le Hello, François-Xavier Weill, Laurent Guillier, Michel-Yves Mistou, Sabrina Cadel-Six   +7 more
doaj   +1 more source

Homologous expression and purification of human HAX‐1 for structural studies

FEBS Open Bio, EarlyView.
This research protocol provides detailed instructions for cloning, expressing, and purifying large quantities of the intrinsically disordered human HAX‐1 protein, N‐terminally fused to a cleavable superfolder GFP, from mammalian cells. HAX‐1 is predicted to undergo posttranslational modifications and to interact with membranes, various cellular ...
Mariana Grieben
wiley   +1 more source

Fatal case of hemolytic-uremic syndrome in an adult due to a rare serogroup O91 Entero hemorrhagic Escherichia coli associated with a Clostridium difficile infection. More than meets the eye

International Journal of Infectious Diseases, 2015
Hemolytic-uremic syndrome due to enterohemorrhagic Escherichia coli, belonging to serogroup O91 has rarely been described. We report here a case of post-diarrheal HUS due to EHEC O91 in an elderly patient for whom diagnosis was delayed given a previously
Thomas Guillard, Anne Limelette, Elisabeth Le Magrex-Debar, Alain Wynckel, Malika Gouali, Patricia Mariani-Kurkdjian, Charlotte Guyot-Colosio, Christophe de Champs   +7 more
doaj   +1 more source

Sex and virulence in Escherichia coli: an evolutionary perspective

Molecular Microbiology, 2006
Pathogenic Escherichia coli cause over 160 million cases of dysentery and one million deaths per year, whereas non‐pathogenic E. coli constitute part of the normal intestinal flora of healthy mammals and birds.
T. Wirth, D. Falush, R. Lan, F. Colles, Patience A Mensa, L. Wieler, H. Karch, P. Reeves, M. Maiden, H. Ochman, M. Achtman   +10 more
semanticscholar   +1 more source

SmallTalk: a novel small‐sized fusion tag for peptide expression and purification

FEBS Open Bio, EarlyView.
The SmallTalk fusion tag allows for the efficient expression and purification of soluble recombinant proteins or peptides in Escherichia coli. Testing with SmallTalk‐GFP confirmed that the proteins were soluble and folded correctly, while SmallTalk‐Bin1b maintained its antimicrobial activity against various bacterial isolates. This streamlined workflow
Atika Tariq, Nestor G. Casillas‐Vega, Alma Gomez‐Loredo, Xristo Zarate   +3 more
wiley   +1 more source

Targets for the MalI repressor at the divergent Escherichia coliK-12malX-malI promoters [PDF]

, 2010
Random mutagenesis has been used to identify the target DNA sites for the MalI repressor at the divergent Escherichia coli K-12 malX-malI promoters. The malX promoter is repressed by MalI binding to a DNA site located from position -24 to position -9 ...
Barnard, Barne, Busby, Chaudhuri, Clausen, El-Robh, Georgina S. Lloyd, Hollands, Jacob, Lloyd, Lodge, Mitchell, Mitchell, Reidl, Reidl, Rita E. Godfrey, Rojo, Stephen J.W. Busby, Swint-Kruse, Valentin-Hansen, Weickert, West, Zdych   +22 more
core   +1 more source

Novel plasmid-mediated colistin resistance mcr-4 gene in Salmonella and Escherichia coli, Italy 2013, Spain and Belgium, 2015 to 2016

Euro surveillance : bulletin Europeen sur les maladies transmissibles = European communicable disease bulletin, 2017
A novel mcr colistin resistance gene was identified in a strain of Salmonella enterica, monophasic variant of serovar Typhimurium (4,5,12:i:- ), isolated from a pig at slaughter in Italy in 2013, and in Escherichia coli strains collected during routine ...
A. Carattoli, L. Villa, C. Feudi, L. Curcio, S. Orsini, A. Luppi, G. Pezzotti, C. Magistrali   +7 more
semanticscholar   +1 more source

dUTPase is essential in zebrafish development and possesses several single‐nucleotide variants with pronounced structural and functional consequences

FEBS Open Bio, EarlyView.
dUTPases are involved in balancing the appropriate nucleotide pools. We showed that dUTPase is essential for normal development in zebrafish. The different zebrafish genomes contain several single‐nucleotide variations (SNPs) of the dut gene. One of the dUTPase variants displayed drastically lower protein stability and catalytic efficiency as compared ...
Viktória Perey‐Simon, Angéla Békesi, Latifa Kazzazy, Jázmin Mihály, Máté Varga, Beáta G. Vértessy, Kinga Nyíri   +6 more
wiley   +1 more source