Results 101 to 110 of about 1,489,227 (363)

Multigene Editing in the Escherichia coli Genome via the CRISPR-Cas9 System

Applied and Environmental Microbiology, 2015
An efficient genome-scale editing tool is required for construction of industrially useful microbes. We describe a targeted, continual multigene editing strategy that was applied to the Escherichia coli genome by using the Streptococcus pyogenes type II ...
Yu Jiang, Biao Chen, Chunlan Duan, Bingbing Sun, Junjie Yang, Sheng Yang   +5 more
semanticscholar   +1 more source

Comparative study of adenosine 3′‐pyrophosphokinase domains of MuF polymorphic toxins

FEBS Open Bio, EarlyView.
With the ultimate goal of understanding the association of toxin‐immunity modules to temperate phages, we characterized toxins from three prophages and examined cross‐protection from immunity proteins. The toxins exhibit adenosine 3′‐pyrophosphokinase activity and are toxic in Escherichia coli.
Eloïse M. Paulet, Julia Bartoli, Atakan Kabtan, Chloé M. Piras, Audrey C. Tempier, Eric Cascales, Julie P. Viala   +6 more
wiley   +1 more source

Dynamic detection of a single bacterium: nonlinear rotation rate shifts of driven magnetic microsphere stages [PDF]

arXiv, 2006
We report on a new technique which was used to detect single Escherichia coli that is based on the changes in the nonlinear rotation of a magnetic microsphere driven by an external magnetic field. The presence of one Escherichia Coli bacterium on the surface of a 2.0 micron magnetic microsphere caused an easily measurable change in the drag of the ...
arxiv  

ATP-Dependent Persister Formation in Escherichia coli

mBio, 2017
Persisters are dormant variants that form a subpopulation of cells tolerant to antibiotics. Persisters are largely responsible for the recalcitrance of chronic infections to therapy.
Yue Shan, Autumn Brown Gandt, Sarah E. Rowe, Julia P. Deisinger, Brian P. Conlon, K. Lewis   +5 more
semanticscholar   +1 more source

Light‐Triggered Protease‐Mediated Release of Actin‐Bound Cargo from Synthetic Cells

Advanced Biology, EarlyView.
TEV Prtoease‐mediated Releasable Actin‐binding Protein (TRAP) is a protein‐based platform consisting of a cargo tightly bound to reconstituted actin networks in synthetic cells which can be proteolyticly released from the bound actin, followed by its secretion through membrane translocation mediated by a cell‐penetrating peptide.
Mousumi Akter, Hossein Moghimianavval, Gary D. Luker, Allen P. Liu   +3 more
wiley   +1 more source

Soluble Toll-Like Receptor 4 Impairs the Interaction of Shiga Toxin 2a with Human Serum Amyloid P Component

Toxins, 2018
Shiga toxin 2a (Stx2a) is the main virulence factor produced by pathogenic Escherichia coli strains (Stx-producing E. coli, STEC) responsible for hemorrhagic colitis and the life-threatening sequela hemolytic uremic syndrome in children.
Maurizio Brigotti, Valentina Arfilli, Domenica Carnicelli, Francesca Ricci, Pier Luigi Tazzari, Gianluigi Ardissino, Gaia Scavia, Stefano Morabito, Xiaohua He   +8 more
doaj   +1 more source

A generalization of Kingman's model of selection and mutation and the Lenski experiment [PDF]

arXiv, 2015
Kingman's model of selection and mutation studies the limit type value distribution in an asexual population of discrete generations and infinite size undergoing selection and mutation. This paper generalizes the model to analyse the long-term evolution of Escherichia. coli in Lenski experiment.
arxiv  

Engineered probiotic Escherichia coli can eliminate and prevent Pseudomonas aeruginosa gut infection in animal models

Nature Communications, 2017
Bacteria can be genetically engineered to kill specific pathogens or inhibit their virulence. We previously developed a synthetic genetic system that allows a laboratory strain of Escherichia coli to sense and kill Pseudomonas aeruginosa in vitro.
In Young Hwang, Elvin Koh, Adison Wong, J. March, W. Bentley, Yung Seng Lee, M. Chang   +6 more
semanticscholar   +1 more source

A Novel Approach to Produce Metal–Metal Composites by Leveraging Immiscibility: Laser Powder Bed Fusion of Nanosilver‐Dispersed Titanium

Advanced Engineering Materials, EarlyView.
Incorporating nanosized silver into titanium for antimicrobial materials exploits the metastable miscibility gap between silver and titanium. Silver nanoparticles are synthesized via pulsed laser ablation in liquid. Silver addition refines the microstructure, increases hardness by 30%, and reduces Staphylococcus capitis microbial adhesion by 70%, with ...
Hamed Shokri, Silja‐Katharina Rittinghaus, Janett Schmelzer, Jessica Bertrand, Bilal Gökce   +4 more
wiley   +1 more source

Pattern formation within Escherichia coli: diffusion, membrane attachment, and self-interaction of MinD molecules [PDF]

, 2004
In E. coli, accurate cell division depends upon the oscillation of Min proteins from pole to pole. We provide a model for the polar localization of MinD based only on diffusion, a delay for nucleotide exchange, and different rates of attachment to the bare membrane and the occupied membrane.
arxiv   +1 more source