Results 81 to 90 of about 546,537 (214)

Lipopolysaccharide uptake is augmented in lipopolysaccharide‐tolerant mouse macrophage‐like cells via increased CD14 expression

FEBS Open Bio, EarlyView.
In normal (nontolerant) cells, CD14 is crucial for both LPS uptake and LPS signaling. In LPS‐tolerant cells, in which LPS‐induced TNF‐α and IFN‐β production is suppressed, there is a dramatic increase in surface CD14 expression. The overexpressed CD14 in LPS‐tolerant cells is responsible for the enhanced LPS uptake without inducing pro‐inflammatory ...
Saeka Nishihara, Takayuki Manabe, Mika Jouta, Kiyoshi Kawasaki   +3 more
wiley   +1 more source

On the viability of Escherichia coli cells lacking DNA topoisomerase I [PDF]

, 2012
Copyright @ 2012 Stockum et al; licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution,
Lloyd, RG, Lloyd Robert G, Rudolph Christian J, Stockum Anna, Christian J Rudolph, Stockum, A, Anna Stockum, Robert G Lloyd, Rudolph, CJ   +8 more
core   +1 more source

Versatile vector tools for efficient protein screening across multiple expression systems

FEBS Open Bio, EarlyView.
A unified vector toolkit enables rapid protein expression screening across E. coli, insect, and mammalian cells. A single primer pair amplifies the target gene, which is inserted into any vector via a standardized interface. This streamlined workflow eliminates repeated cloning steps, accelerating the identification of optimal expression conditions for
Zhimin Zhu, Yaqing Liu, Lei Qin, Feng Yu, Qisheng Wang, Yahui Liu   +5 more
wiley   +1 more source

Mutation rate plasticity in rifampicin resistance depends on Escherichia coli cell–cell interactions [PDF]

, 2014
Variation of mutation rate at a particular site in a particular genotype, in other words mutation rate plasticity (MRP), can be caused by stress or ageing. However, mutation rate control by other factors is less well characterized.
John A. D. Aston, Aston, Elizabeth, Christopher G. Knight, Roman V. Belavkin, Knight, Christopher G., Aston, JA, Aston, John A D, Rash, BM, Knight, C. G. (Christopher G.), Elizabeth Aston, Channon, A, Krašovec, Rok; id_orcid, Knight, CG, An Kadirvel, Aston, John A. D., Forbes, S, Alastair Channon, Channon, Alastair, Knight, Christopher G.; id_orcid, Aston, E, Krašovec, Rok, Belavkin, RV, Forbes, Sarah, Aston, John A.D., Sarah Forbes, Kadirvel, Manikandan, Belavkin, Roman V., Bharat M. Rash, Kadirvel, Manikandan; id_orcid, Rash, Bharat M., Krašovec, R, Kadirvel, M, Channon, A., Belavkin, R., Krašovec, R., Aston, E., Forbes, S., Kadirvel, M., Rash, B., Knight, C., Aston, J.   +40 more
core   +1 more source

The crystal structure of the Borrelia burgdorferi nicotinamidase BBE22 resolves a long‐standing annotation error

FEBS Open Bio, EarlyView.
The crystal structure of Borrelia burgdorferi nicotinamidase (PncA/BBE22) reveals the correct full‐length protein initiated from a non‐canonical AUU start codon. The structure validates previous biochemical findings and resolves a long‐standing annotation error, demonstrating that the truncated database sequence is structurally incompatible with the ...
Kalvis Brangulis
wiley   +1 more source

Molecular characterization of covRS mutations in M1UK Streptococcus pyogenes

FEBS Open Bio, EarlyView.
Group A Streptococcus (GAS) acquires covRS mutations driving a hypervirulent bacterial state, frequently associated with invasive disease‐like necrotizing fasciitis. We demonstrate that the newly emerged M1UK GAS lineage can also acquire these mutations.
Jarrad Pritchard, Emma‐Jayne Proctor, Jiawa Wang, Nikolas P. Johnston, Andrew Hayes, Georgia McCorkell, Haibo Yu, Jai Tree, Mark R. Davies, Mark J. Walker, Ronald Sluyter, Stephan Brouwer, Martina L. Sanderson‐Smith   +12 more
wiley   +1 more source

The lytB gene of Escherichia coli is essential and specifies a product needed for isoprenoid biosynthesis [PDF]

, 2001
LytB and GcpE, because they are codistributed with other pathway enzymes, have been predicted to catalyze unknown steps in the nonmevalonate pathway for isoprenoid biosynthesis.
McLennan, Neil, Coulson, Andrew, McAteer, Sean, Masters, Millicent/Mrs Donachie   +3 more
core  

Transcripts enriched in codons that trigger P‐site tRNA‐mediated mRNA decay possess stable mRNA

FEBS Open Bio, EarlyView.
PTMD codons were first described by Mendel et al. as mediators of an mRNA decay pathway dependent on the human protein CNOT3, homologous to yeast Not5. Our findings confirm that PTMD codons destabilize transcripts; however, unlike in yeast, the human pathway specifically targets and slightly destabilizes primarily stable mRNAs.
Rodolfo Lopes Carneiro, Fernando Lucas Palhano   +1 more
wiley   +1 more source

Remission of histiocytic ulcerative colitis in Boxer dogs correlates with eradication of invasive intramucosal Escherichia coli [PDF]

, 2009
BACKGROUND: Historically, histiocytic ulcerative (HUC) (or granulomatous) colitis of Boxer dogs was considered an idiopathic immune-mediated disease with a poor prognosis. Recent reports of dramatic responses to enrofloxacin and the discovery of invasive
Dogan, B., Nicholls, P.K., Simpson, K.W., Davies, D.R., James, F.E., MacDonough, S.P., Mansfield, C.S., Craven, M., O'Hara, A.J.   +8 more
core  

Cloning and phenotypic expression in Escherichia coli of a Bacillus subtilis gene fragment coding for sucrose hydrolysis [PDF]

, 1986
Friehs K, Schörgendorfer K, Schwab H, Lafferty RM. Cloning and phenotypic expression in Escherichia coli of a Bacillus subtilis gene fragment coding for sucrose hydrolysis. Journal of Biotechnology.
Lafferty, R. M., Friehs, Karl, Schörgendorfer, K., Schwab, H.   +3 more
core   +1 more source