Escherichia coli Protein Expression System for Acetylcholine Binding Proteins (AChBPs). [PDF]
PLoS ONE, 2016 Nicotinic acetylcholine receptors (nAChR) are ligand gated ion channels, identified as therapeutic targets for a range of human diseases. Drug design for nAChR related disorders is increasingly using structure-based approaches.
Nikita Abraham +3 more
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Protein Mobility in the Cytoplasm of Escherichia coli [PDF]
Journal of Bacteriology, 1999 ABSTRACT The rate of protein diffusion in bacterial cytoplasm may constrain a variety of cellular functions and limit the rates of many biochemical reactions in vivo. In this paper, we report noninvasive measurements of the apparent diffusion coefficient of green fluorescent protein (GFP) in the cytoplasm of ...
Elowitz, Michael B. +4 more
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Expression and Purification of the Arabidopsis E4 SUMO Ligases PIAL1 and PIAL2
Bio-Protocol, 2015 The proteins PIAL1 (At1g08910) and PIAL2 (At5g41580) are members of the recently discovered group of plant E4 SUMO ligases. This protocol allows quick and simple expression of the recombinant proteins in Escherichia coli (E.
Konstantin Tomanov, Andreas Bachmair
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Acid shock proteins of Escherichia coli [PDF]
FEMS Microbiology Letters, 1990 Synthesis of total cellular proteins of Escherichia coli was studied after transfer of cultures from pH 6.9 to pH 4.3. Proteins induced by such an external pH shift down were identified by mono- and bi-dimensional electrophoresis. 30 to 45 min after an acid shift, a group of at least sixteen polypeptides was markedly induced. Four of these polypeptides
Raymond Portalier, Martine Heyde
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Export of functional Streptomyces coelicolor alditol oxidase to the periplasm or cell surface of Escherichia coli and its application in whole-cell biocatalysis [PDF]
, 2009 Streptomyces coelicolor A3(2) alditol oxidase (AldO) is a soluble monomeric flavoprotein in which the flavin cofactor is covalently linked to the polypeptide chain.
AJ Driessen +44 more
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Genes and proteins of Escherichia coli (GenProtEc) [PDF]
Nucleic Acids Research, 1996 GenProtEC is a database of Escherichia coli genes and their gene products, classified by type of function and physiological role and with citations to the literature for each. Also present are data on sequence similarities amongE.coliproteins with PAM values, percent identity of amino acids, length of alignment and percent aligned.
M, Riley, D B, Space
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Isolation of Inner Membrane Vesicles from Escherichia coli by Using an Affinity Tag
Bio-Protocol, 2012 This protocol was developed in a project aimed to identify the inner membrane proteins localizing to cell poles in Escherichia coli (E. coli). By using a known polar protein Tar as a tag, we isolated pole-derived inner membrane vesicles by affinity ...
Gang Li, Kevin Young
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Mistranslated protein in Escherichia coli.
Journal of Biological Chemistry, 1981 Amino acid starvation of a variety of different types of cells has been reported to induce protein degradation and also specific mistranslation. For certain amino acid starvations, the mistranslated protein, which contains specific amino acid substitutions, can be separated and quantified by two-dimensional polyacrylamide gel electrophoresis.
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High-Affinity Phosphate-Binding Protein (PBP) For Phosphorous Recovery: Proof of Concept Using Recombinant \u3cem\u3eEscherichia coli\u3c/em\u3e [PDF]
, 2016 Phosphorus (P) is a critical, non-renewable nutrient; yet excess discharges can lead to eutrophication and deterioration of water quality. Thus, P removal from water must be coupled with P recovery to achieve sustainable P management. P-specific proteins
Ballent, Wendy, Mayer, Brooke, Yang, Yu
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DNA Sampling: a method for probing protein binding at specific loci on bacterial chromosomes [PDF]
, 2009 We describe a protocol, DNA sampling, for the rapid isolation of specific segments of DNA, together with bound proteins, from Escherichia coli K-12. The DNA to be sampled is generated as a discrete fragment within cells by the yeast I-SceI meganuclease ...
Blattner +29 more
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