Results 81 to 90 of about 762,581 (360)

Cell wall target fragment discovery using a low‐cost, minimal fragment library

FEBS Letters, EarlyView.
LoCoFrag100 is a fragment library made up of 100 different compounds. Similarity between the fragments is minimized and 10 different fragments are mixed into a single cocktail, which is soaked to protein crystals. These crystals are analysed by X‐ray crystallography, revealing the binding modes of the bound fragment ligands.
Kaizhou Yan, Mathew Stanley, Olawale Raimi, Andrew T. Ferenbach, Helge C Dorfmueller, Daan M. F. van Aalten   +5 more
wiley   +1 more source

Comparison of bacterial intracellular and secreted proteins produced in milk versus medium for Escherichia coli by proteomic analysis

Journal of Dairy Science
: The growth and reproduction of microorganisms are dependent on nutrient supply. Here, milk and Luria-Bertani (LB) media were used as nutrition sources for Escherichia coli, and the changes in bacterial and secretory proteins at 3 time points (3, 9, and
Qiyue Sun, Yanxin Li, Hongning Jiang, Qijing Du, Jun Wang, Rongbo Fan, Rongwei Han, Yongxin Yang   +7 more
doaj   +1 more source

Identifying New Small Proteins in Escherichia coli [PDF]

PROTEOMICS, 2018
Abstract The number of small proteins (SPs) encoded in the Escherichia coli genome is unknown, as current bioinformatics and biochemical techniques make short gene and small protein identification challenging.
Caitlin E. VanOrsdel, John P. Kelly, Brittany N. Burke, Christina D. Lein, Christopher E. Oufiero, Joseph F. Sanchez, Larry E. Wimmers, David J. Hearn, Fatimeh J. Abuikhdair, Kathryn R. Barnhart, Michelle L. Duley, Sarah E. G. Ernst, Briana A. Kenerson, Aubrey J. Serafin, Matthew R. Hemm   +14 more
openaire   +2 more sources

Analysis of ligation and DNA binding by Escherichia coli DNA ligase (LigA). [PDF]

, 2005
NAD+-dependent DNA ligases are essential enzymes in bacteria, with the most widely studied of this class of enzymes being LigA from Escherichia coli. NAD+-dependent DNA ligases comprise several discrete structural domains, including a BRCT domain at the ...
Adam Wilkinson, Alexandra Bonner, Altschul, Andrew Hemmings, Andrew Smith, Baer, Bork, Brotz-Oesterhelt, Callebaut, Derbyshire, Dermody, Desmond Bullard, Doherty, Doherty, Doherty, Feng, Gajiwala, Georlette, Georlette, Glover, Gong, Grawunder, Guex, Gunther, Halligan, Heather Sayer, Herrmann, Huyton, Jeon, Joo, Kaczmarek, Kodama, Krishnan, Lavesa-Curto, Lee, Legrain, Lehman, Manuel Lavesa-Curto, Masson, Odell, Pascal, Richard Bowater, Sambrook, Shuman, Singleton, Sriskanda, Sriskanda, Sriskanda, Sriskanda, Subramanya, Timson, Timson, Wieland, Wilkinson, Wilkinson, Williams, Yamane, Yamane, Zhang, Zimmerman   +59 more
core   +1 more source

Type III Secretion Effectors with Arginine N-Glycosyltransferase Activity [PDF]

, 2020
Type III secretion systems are used by many Gram-negative bacterial pathogens to inject proteins, known as effectors, into the cytosol of host cells. These virulence factors interfere with a diverse array of host signal transduction pathways and cellular
Araujo Garrido, Juan Luis, Bernal Bayard, Joaquín, Ramos Morales, Francisco   +2 more
core   +1 more source

A general model for analysis of linear and hyperbolic enzyme inhibition mechanisms

FEBS Open Bio, EarlyView.
We developed a general enzyme kinetic model that integrates these six basic inhibition mechanism onto a single one. From this model, we deduced a general enzyme kinetic equation that through modulation of simple parameters, γ (the relative inhibitor affinity for two binding sites) and β (the reactivity of the enzyme–substrate–inhibitor complex), is ...
Rafael S. Chagas, Sandro R. Marana
wiley   +1 more source

Rainbow Vectors for Broad-Range Bacterial Fluorescence Labeling.

PLoS ONE, 2016
Since their discovery, fluorescent proteins have been widely used to study protein function, localization or interaction, promoter activity and regulation, drug discovery or for non-invasive imaging.
Mariette Barbier, F Heath Damron
doaj   +1 more source

Remarkable diversity in the enzymes catalyzing the last step in synthesis of the pimelate moiety of biotin. [PDF]

PLoS ONE, 2012
Biotin synthesis in Escherichia coli requires the functions of the bioH and bioC genes to synthesize the precursor pimelate moiety by use of a modified fatty acid biosynthesis pathway.
Madelyn M Shapiro, Vandana Chakravartty, John E Cronan   +2 more
doaj   +1 more source

Tuning Escherichia coli for membrane protein overexpression [PDF]

Proceedings of the National Academy of Sciences, 2008
A simple generic method for optimizing membrane protein overexpression in Escherichia coli is still lacking. We have studied the physiological response of the widely used “Walker strains” C41(DE3) and C43(DE3), which are derived from BL21(DE3), to membrane protein overexpression.
Wagner, Samuel, Klepsch, Mirjam M., Schlegel, Susan, Appel, Ansgar, Draheim, Roger, Tarry, Michael, Hogbom, Martin, van Wijk, Klaas J., Slotboom, Dirk J., Persson, Jan O., de Gier, Jan-Willem, Högbom, Martin   +11 more
openaire   +2 more sources

Homologous expression and purification of human HAX‐1 for structural studies

FEBS Open Bio, EarlyView.
This research protocol provides detailed instructions for cloning, expressing, and purifying large quantities of the intrinsically disordered human HAX‐1 protein, N‐terminally fused to a cleavable superfolder GFP, from mammalian cells. HAX‐1 is predicted to undergo posttranslational modifications and to interact with membranes, various cellular ...
Mariana Grieben
wiley   +1 more source