Results 81 to 90 of about 1,276,365 (260)

Global functional atlas of Escherichia coli encompassing previously uncharacterized proteins.

PLoS Biology, 2009
One-third of the 4,225 protein-coding genes of Escherichia coli K-12 remain functionally unannotated (orphans). Many map to distant clades such as Archaea, suggesting involvement in basic prokaryotic traits, whereas others appear restricted to E.
Pingzhao Hu, Sarath Chandra Janga, Mohan Babu, J Javier Díaz-Mejía, Gareth Butland, Wenhong Yang, Oxana Pogoutse, Xinghua Guo, Sadhna Phanse, Peter Wong, Shamanta Chandran, Constantine Christopoulos, Anaies Nazarians-Armavil, Negin Karimi Nasseri, Gabriel Musso, Mehrab Ali, Nazila Nazemof, Veronika Eroukova, Ashkan Golshani, Alberto Paccanaro, Jack F Greenblatt, Gabriel Moreno-Hagelsieb, Andrew Emili   +22 more
doaj   +1 more source

SHuffle, a novel Escherichia coli protein expression strain capable of correctly folding disulfide bonded proteins in its cytoplasm

Microbial Cell Factories, 2012
BackgroundProduction of correctly disulfide bonded proteins to high yields remains a challenge. Recombinant protein expression in Escherichia coli is the popular choice, especially within the research community.
J. Lobstein, C. Emrich, C. Jeans, M. Faulkner, P. Riggs, M. Berkmen   +5 more
semanticscholar   +1 more source

Controlled functional expression of the bacteriocins pediocin PA-1 and bactofencin A in Escherichia coli [PDF]

, 2017
peer-reviewedThe bacteriocins bactofencin A (class IId) and pediocin PA-1 (class IIa) are encoded by operons with a similarly clustered gene organization including a structural peptide, an immunity protein, an ABC transporter and accessory bacteriocin ...
Cotter, Paul D., Hill, Colin, Mesa-Pereira, Beatriz, O’Connor, Paula M., Rea, Mary, Ross, R Paul   +5 more
core   +2 more sources

Immunolabeling of Proteins in situ in Escherichia coli K12 Strains

Bio-Protocol, 2013
This protocol was developed to label proteins in bacterial cells with antibodies conjugated to a fluorophore for fluorescence microscopy imaging. The procedure is optimized to minimize morphological changes and also to minimize the amount of antibodies ...
Nienke Buddelmeijer, Mirjam Aarsman, Tanneke den Blaauwen   +2 more
doaj   +1 more source

Tomato EF-Tsmt, a functional mitochondrial translation elongation factor from higher plants [PDF]

, 2003
Ethylene-induced ripening in tomato (Lycopersicon esculentum) resulted in the accumulation of a transcript designated LeEF-Tsmt that encodes a protein with significant homology to bacterial Ts translational elongation factor (EF-Ts).
Benichou, Mohamed, Bouzayen, Mondher, Chaves, Ana, Delalande, Corinne, Jauneau, Alain, Latché, Alain, Li, Zhengguo, Pech, Jean-Claude, Spremulli, Linda L., Tournier, Barthélémy, Zegzouti, Hicham   +10 more
core   +1 more source

High-resolution structure of the Escherichia coli ribosome

Nature Structural &Molecular Biology, 2015
Protein synthesis by the ribosome is highly dependent on the ionic conditions in the cellular environment, but the roles of ribosome solvation have remained poorly understood.
J. Noeske, Michael R. Wasserman, Daniel S. Terry, R. Altman, S. Blanchard, J. Cate   +5 more
semanticscholar   +1 more source

Loss of β-ketoacyl acyl carrier protein synthase III activity restores multidrug-resistantEscherichia colisensitivity to previously ineffective antibiotics [PDF]

, 2022
Yaoqin Hong, Jilong Qin, Anthony D. Verderosa, Sophia Hawas, Bing Zhang, Mark A. T. Blaskovich, John E. Cronan, Makrina Totsika   +7 more
openalex   +1 more source

ruvA Mutants that resolve Holliday junctions but do not reverse replication forks [PDF]

, 2008
RuvAB and RuvABC complexes catalyze branch migration and resolution of Holliday junctions (HJs) respectively. In addition to their action in the last steps of homologous recombination, they process HJs made by replication fork reversal, a reaction which ...
A Blastyak, A Constantinou, A Kuzminov, A Machwe, Alison Sylvia Bradley, B Guenther, B Michel, B Michel, Bénédicte Michel, C Ralf, CA Parsons, CE Shurvinton, CV Privezentzev, CZ Bachrati, D Chamberlain, D Hargreaves, DV Bugreev, EC Friedberg, EP Rocha, F Lecointe, F Osman, F Osman, G Grompone, H Iwasaki, H Shinagawa, IR Tsaneva, IR Tsaneva, Irina Tsaneva, JB Rafferty, JH Miller, JK Karow, JR Donaldson, K Hiom, K Yamada, K Yamada, L Wu, M Ariyoshi, M Fromant, M Lopes, M Oram, M Seigneur, Marie Le Masson, MC Whitby, MC Whitby, ME Robu, MJ Flores, P McGlynn, P McGlynn, P McGlynn, P McGlynn, R Lestini, RC Heller, RG Lloyd, SC West, SD Vincent, SM Roe, SN Chan, T Hishida, T Hishida, T Nishino, T Nishino, T Shibata, TN Mandal, W Bussen, William F. Burkholder, Y Liu, Y Liu, Z Baharoglu, Zeynep Baharoglu   +68 more
core   +3 more sources

MCE domain proteins: conserved inner membrane lipid-binding proteins required for outer membrane homeostasis

Scientific Reports, 2017
Bacterial proteins with MCE domains were first described as being important for Mammalian Cell Entry. More recent evidence suggests they are components of lipid ABC transporters.
Georgia L. Isom, Nathaniel J. Davies, Zhi-Soon Chong, Jack A. Bryant, Mohammed Jamshad, Maria Sharif, Adam F. Cunningham, Timothy J. Knowles, Shu-Sin Chng, Jeffrey A. Cole, Ian R. Henderson   +10 more
doaj   +1 more source

Structural genomics of human proteins – target selection and generation of a public catalogue of expression clones

Microbial Cell Factories, 2005
Background The availability of suitable recombinant protein is still a major bottleneck in protein structure analysis. The Protein Structure Factory, part of the international structural genomics initiative, targets human proteins for structure ...
Harttig Ulrich, Sievert Volker, Scheich Christoph, Büssow Konrad, Schultz Jörg, Simon Bernd, Bork Peer, Lehrach Hans, Heinemann Udo   +8 more
doaj   +1 more source