Results 181 to 190 of about 3,195,499 (217)

Characterization of EST-SSRs in loblolly pine and spruce

Tree Genetics & Genomes, 2006
In the first large study of conifer expressed sequence tag-simple sequence repeats (EST-SSRs), two large conifer EST databases were characterized for EST-SSRs. One database was from “interior spruce” (white and Engelmann spruce in Southern British Columbia) and Sitka spruce, while the other was from loblolly pine.
Yanik Bérubé, Jun Zhuang, Dainis Rungis, Steven Ralph, Jörg Bohlmann, Kermit Ritland   +5 more
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Characterization of EST–SSRs from Cryptomeria japonica

Conservation Genetics Resources, 2009
Simple sequence repeat (SSR) markers were developed for Cryptomeria japonica from 55,530 expressed sequence tags (ESTs). Of the 219 designed primer pairs, 176 showed clear PCR amplification products and 27 of these revealed polymorphisms in six individuals sampled at sites across Japan.
Yoshinari Moriguchi, Saneyoshi Ueno, Tokuko Ujino-Ihara, Norihiro Futamura, Asako Matsumoto, Kenji Shinohara, Yoshihiko Tsumura   +6 more
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EST-SSR Markers fromHeteroderaglycinesIchinohe

Генетика, 2014
The soybean cyst nematode Heterodera glycines Ichinohe is a severe agricultural pest for which genetic resources are limited. In this study, 295 simple sequence repeats (SSRs) were identified from 259 expressed sequenced tags (ESTs), Which were selected from 9,443 unigenes. The successful primer pairs were designed against six regions.
H M, Wang, H H, Zhao, C Z, Zhao, D, Chu
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Exploitation of pepper EST–SSRs and an SSR-based linkage map

Theoretical and Applied Genetics, 2006
As genome and cDNA sequencing projects progress, a tremendous amount of sequence information is becoming publicly available. These sequence resources can be exploited for gene discovery and marker development. Simple sequence repeat (SSR) markers are among the most useful because of their great variability, abundance, and ease of analysis. By in silico
Gibum, Yi, Je Min, Lee, Sanghyeob, Lee, Doil, Choi, Byung-Dong, Kim   +4 more
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Development of EST-SSR in foxtail millet (Setaria italica)

Genetic Resources and Crop Evolution, 2007
The development of EST-SSR in foxtail millet (Setaria italica) for polymorphism and transferability study was reported here. From 1213 EST sequences, 30 SSRs were obtained and primers were designed for 26 SSRs. Among them, four pairs of SSR primers amplified polymorphic products in 12 foxtail millet cultivars and one accession of Setaria viridis, a ...
Tian-Yu Wang, Guo-Ying Wang
exaly   +2 more sources

Construction of new EST-SSRs for Fusarium resistant wheat breeding

Computational Biology and Chemistry, 2017
Surveying Fusarium resistance in wheat with easy applicable molecular markers such as simple sequence repeats (SSRs) is a prerequest for molecular breeding. Expressed sequence tags (ESTs) are one of the main sources for development of new SSR candidates. Therefore, 18.292 publicly available wheat ESTs were mined and genotyping of newly developed 55 EST-
Aysen Yumurtaci, Hulya Sipahi, Ayed Al-Abdallat, Abdulqader Jighly, Michael Baum 0001   +4 more
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Development and analysis of EST-SSRs for flax (Linum usitatissimum L.)

Theoretical and Applied Genetics, 2009
A set of 146,611 expressed sequence tags (ESTs) were generated from 10 flax cDNA libraries. After assembly, a total of 11,166 contigs and 11,896 singletons were mined for the presence of putative simple sequence repeats (SSRs) and yielded 806 (3.5%) non-redundant sequences which contained 851 putative SSRs. This is equivalent to one EST-SSR per 16.5 kb
Cloutier, Sylvie, Niu, Zhixia, Datla, Raju, Duguid, Scott   +3 more
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Study of EST-SSR marker system of Cordyceps

China Journal of Chinese Materia Medica, 2011
Objective To establish the EST-SSR marker system for Cordyceps by using ESTs of C. bassiana and C. militaris. Method The ESTs of Cordyceps were downloaded from the public database of NCBI, and the redundant ESTs with low quality were removed. The EST-SSR primers were designed by Sequece Seiner 1. 2.
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Development of EST-SSR primers and their practicability test for Laminaria

Acta Oceanologica Sinica, 2011
Laminaria (Saccharina) is one of the economic important seaweeds. Through sequence analysis of 4 099 ESTs from Laminaria (971 were generated from our own cDNA laboratories and 3 128 were downloaded from updated EST databases) with SSRIT software, two hundred and fifty-four SSRs in 206 ESTs were found.
Guoliang Wang, Xiaolong Tan, Junling Shen, Jian Li, Lei Zhang, Jianwei Sun, Bin Wang, Manli Weng, Tao Liu   +8 more
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[EST-SSR identification of Lonicera japonica Thunb].

Yao xue xue bao = Acta pharmaceutica Sinica, 2013
Simple and effective methods are needed for the identification of Chinese medicinal material species and their variety. Lonicera japonica Thunb. is one of Chinese herbal medicines widely demanded. A total of 3 705 EST-SSRs of L. japonica and 2 818 EST-SSRs of L. japonica var. chinensis Thunb. were identified from EST database in our lab.
Chao, Jiang, Yuan, Yuan, Gui-Ming, Liu, Lu-Qi, Huang, Xu-Min, Wang, Jun, Yu, Min, Chen   +6 more
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