Results 71 to 80 of about 1,210,251 (303)

Mycobacterial cell division arrest and smooth‐to‐rough envelope transition using CRISPRi‐mediated genetic repression systems

FEBS Open Bio, EarlyView.
CRISPRI‐mediated gene silencing and phenotypic exploration in nontuberculous mycobacteria. In this Research Protocol, we describe approaches to control, monitor, and quantitatively assess CRISPRI‐mediated gene silencing in M. smegmatis and M. abscessus model organisms.
Vanessa Point, Wafaa Achache, Janïs Laudouze, Eliana Sepulveda Ramos, Mickaël Maziero, Céline Crauste, Stéphane Canaan, Pierre Santucci   +7 more
wiley   +1 more source

dUTPase is essential in zebrafish development and possesses several single‐nucleotide variants with pronounced structural and functional consequences

FEBS Open Bio, EarlyView.
dUTPases are involved in balancing the appropriate nucleotide pools. We showed that dUTPase is essential for normal development in zebrafish. The different zebrafish genomes contain several single‐nucleotide variations (SNPs) of the dut gene. One of the dUTPase variants displayed drastically lower protein stability and catalytic efficiency as compared ...
Viktória Perey‐Simon, Angéla Békesi, Latifa Kazzazy, Jázmin Mihály, Máté Varga, Beáta G. Vértessy, Kinga Nyíri   +6 more
wiley   +1 more source

Elimination of Toxic Solvents from Analytical Methods in Food Analysis: Caffeine Determination in Tea as an Example

Foods
This study presents an innovative method for caffeine determination in tea, employing ethanol as the sole organic solvent for both SPE sample preparation and chromatographic analysis.
Oktawia Kalisz, Aleksandra Jaworska, Sylwia Studzińska, Szymon Bocian   +3 more
doaj   +1 more source

pH Indicator Paper by Immobilizing Turmeric Rhizome Ethanol Extract on Filter Paper [PDF]

, 2022
Maria Aloisia Uron Leba, Maria Benedikta Tukan, Faderina Komisia   +2 more
openalex   +1 more source

Tumor‐stromal crosstalk and macrophage enrichment are associated with chemotherapy response in bladder cancer

FEBS Open Bio, EarlyView.
Chemoresistance in bladder cancer: Macrophage recruitment associated with CXCL1, CXCL5 and CXCL8 expression is characteristic of Gemcitabine/Cisplatin (Gem/Cis) Non‐Responder tumors (right side) while Responder tumors did not show substantial tumor‐stromal crosstalk (left side). All biological icons are attributed to Bioicons: carcinoma, cancerous‐cell‐
Sophie Leypold, Janik Riese, Lancelot Seillier, Mark Kühnel, Julia Pannhausen, Charlotte O. J. Fröhlich, Christian Martin, Peter Boor, Matthias Saar, Danny D. Jonigk, Nadine T. Gaisa, Michael Rose   +11 more
wiley   +1 more source

In Situ-Derived N-Doped ZnO from ZIF-8 for Enhanced Ethanol Sensing in ZnO/MEMS Devices

Molecules
Microelectromechanical systems (MEMS) gas sensors have numerous advantages such as compact size, low power consumption, ease of integration, etc., while encountering challenges in sensitivity and high resistance because of their low sintering temperature.
Meihua Liang, Yong Yan, Jiaxuan Yang, Xiaodong Liu, Rongrong Jia, Yuanyuan Ge, Zhili Li, Lei Huang   +7 more
doaj   +1 more source

HIV‐1 establishes immediate latency in T cells expressing the viral Nef protein

FEBS Open Bio, EarlyView.
Nef is a viral protein often omitted from HIV‐1 reporter viruses. Consequently, its role in viral latency is unclear. We developed three novel dual reporter HIV‐1 derivatives that express Nef and allow for detection of latent and productive infection. Using these reporters, we show that Nef does not affect the establishment of immediate viral latency ...
Cindy Lam, Ivan Sadowski
wiley   +1 more source

U.S. International Agricultural Trade Policy: Interests, Institutions and Information in the Corn Supply Chain [PDF]

Brazilian Political Science Review, 2016
The purpose of this article is to analyze the U.S. international agricultural trade policy by focusing on instruments and institutional arrangements. Policy decision-making is analyzed by means of three variables: 1) how interests are mobilized; 2) how ...
Laís Forti Thomaz, Marcelo Fernandes de Oliveira   +1 more
doaj  

Suppressive effect of melatonin administration on ethanol‐induced gastroduodenal injury in rats in vivo [PDF]

, 1997
Daniela Melchiorri, Ewa Sewerynek, R. J. Reiter, Genaro Gabriel Ortíz, Burkhard Pöeggeler, Giuseppe Nisticò   +5 more
openalex   +1 more source

Alcohol‐induced altered glycans in human tracheal epithelial cells promote bacterial adhesion

FEBS Open Bio, EarlyView.
Alcohol induces altered glycans to promote bacteria adhesion. Heavy alcohol drinking is known to increase the risk of bacterial pneumonia. However, the link between alcohol levels and risk of infection remains underexplored. Recently, we found that alcohol induced α2‐6sialo mucin O‐glycans in human tracheobronchial epithelial cells, which mediated the ...
Pi‐Wan Cheng, Souvik Datta, Derrick R. Samuelson   +2 more
wiley   +1 more source