Flow Cytometric Analysis of Efflux by Dye Accumulation
Gram-negative infections are increasingly difficult to treat because of their impermeable outer membranes (OM) and efflux pumps which maintain a low intracellular accumulation of antibiotics within cells.
Emily E. Whittle +5 more
doaj +1 more source
G-banding of plant chromosomes [PDF]
A method for the staining of the chromosomal material of plant cells so as to induce G-Bands, useful in plant genetic studies such as hydridization. G-Banding is induced by contacting living plant cells, such as those of plants of the family Gramineae ...
Hsu, Tao-Chiuh
core +1 more source
Single Cell Analysis of Human RAD18-Dependent DNA Post-Replication Repair by Alkaline Bromodeoxyuridine Comet Assay [PDF]
Damage to DNA can block replication progression resulting in gaps in the newly synthesized DNA. Cells utilize a number of post-replication repair (PRR) mechanisms such as the RAD18 controlled translesion synthesis or template switching to overcome the ...
Downes, C. Stephen +4 more
core +2 more sources
Fluorescence microscopy methods for the determination of somatic cell count in raw cow's milk
The direct fluorescence microscopy method with ethidium bromide staining can be used for somatic cell counting in raw cow's milk. However, this method has some limitations that may influence the results of the analysis.
P. Zajac +3 more
doaj +1 more source
Caffeine-induced release of small molecules from DNA nanostructures
Summary: Several planar aromatic molecules are known to intercalate between base pairs of double-stranded DNA. This mode of interaction has been used to stain DNA as well as to load drug molecules onto DNA-based nanostructures.
Bharath Raj Madhanagopal +3 more
doaj +1 more source
Functional Analysis of in Vivo and in Organello Footprinting of HeLa Cell Mitochondrial DNA in Relationship to ATP and Ethidium Bromide Effects on Transcription [PDF]
In vivo and in organello footprinting techniques based on methylation interference have been utilized to investigate protein-DNA interactions in the transcription initiation and rDNA transcription termination regions of human mitochondrial DNA (mtDNA) in
Attardi, Giuseppe +2 more
core +1 more source
The effect of intrahippocampal injection of estradiol on spatial memory in male rats during multiple sclerosis disease [PDF]
Background & Objective: Multiple sclerosis (MS) is a demyelinating disease of central nervous system, which is approximately two to three times more common in women than men. It was suggested that sexual hormones affect MS disease.
Vahideh Zarrin +2 more
doaj +2 more sources
Synthesis and Neuropharmacological Evaluation of Some Novel Quinoxaline 2, 3-Dione Derivatives
Quinoxaline-2, 3-dione obtained from cyclocondensation reaction of o-phenylene diamine with oxalic acid was reacted with three different ketones and formaldehyde to give the corresponding Mannich bases in satisfactory yield.
Selvaraj Jubie +2 more
doaj +1 more source
Modulation of 5-Aminolevulinic acid mediated photodynamic therapy induced cell death in a human lung adenocarcinoma cell line [PDF]
Photodynamic therapy (PDT) is a cancer treatment involving the administration of a photosensitising drug which selectively accumulates in tumor tissue, followed by irradiation with appropriate wavelength light.
Battle, A. +3 more
core +2 more sources
Ethidium bromide binding to DNA cryogels
Abstract The interaction of the classical intercalator ethidium bromide (EtBr) with the double helical network strands of DNA cryogels was investigated. The cryogels were prepared starting from aqueous solutions of DNA (about 2000 base pairs long) at −18 °C using 1,4-butanediol diglycidyl ether crosslinker under various reaction conditions.
Karacan, Pinar, Okay, Oguz
openaire +2 more sources

