Results 121 to 130 of about 5,903,550 (301)

Electroporation of nucleic acids into prokaryotic and eukaryotic cells by square wave pulses

, 1998
A novel electroporator is presented that generates adjustable square wave pulses with a maximal pulse strength of 2500 V / 100 A and a pulse length between 0.1 and 15 ms and that efficiently transfects prokaryotic and eukaryotic cells.
Worgall, S., Rascher, W., Koenen, M., Hänze, J., Fischer, L.   +4 more
core   +1 more source

The C‐terminal domain of yeast Arginyltransferase1 is essential for its catalytic activity

FEBS Open Bio, EarlyView.
Arginyltransferase 1 (Ate1), a eukaryotic enzyme, catalyses arginylation, transferring arginine from tRNA‐Arg to the amino terminus of the target protein. Overexpression of Ate1 in yeast is lethal and is dependent on arginylation. This study elucidates how mutations in the cofactor‐binding and active site of Ate1 and truncation of its structural ...
Vikas Kumar Yadav, Shweta Shweta, Manisha Malhotra, Vikash Maurya, Akhilesh Kumar   +4 more
wiley   +1 more source

Construction of A Eukaryotic Expression Vector Carrying the iNOS Gene and Its Effect on A549 Lung Cancer Cells

Chinese Journal of Lung Cancer, 2012
Background and objective The iNOS gene is associated with NO-mediated antitumor effects. The aims of this study are to construct a eukaryotic expression plasmid that carries the iNOS gene and to detect the expression levels and antitumor effects of the ...
Sujuan YE, Weihan YANG, Yu WANG, Wenjing OU, Qingping MA, Wen ZHU   +5 more
doaj   +1 more source

Genetic dissection of human ABCE1 in yeast reveals separable requirements for ribosome recycling and suppression of aberrant reinitiation

FEBS Open Bio, EarlyView.
Human ABCE1 cannot functionally replace its yeast ortholog. Yeast–human chimera analysis identified NBD1 as a major interspecies barrier. Genetic screening yielded hABCE1 revertants that rescue yeast viability but fail to suppress aberrant translation reinitiation in the 3′ UTR.
Eriko Nakata, Yuxin Li, Kei Endo, Koichi Ito   +3 more
wiley   +1 more source

The fraction of the non-viable eukaryotic cells quantified by flow cytometry versus automatic analysis with BioFilmAnalyzer.

, 2018
The fraction of the non-viable eukaryotic cells quantified by flow cytometry versus automatic analysis with BioFilmAnalyzer.
Vladimir Yu Volkov (5159051), Oleg A. Markelov (5159054), Irshad S. Sharafutdinov (5159057), Regina R. Murtazina (5159045), Elena Yu Trizna (5159039), Pavel V. Zelenikhin (5159048), Diana R. Baydamshina (5159042), Airat R. Kayumov (667391), Vladislav Melnikov (5159036), Mikhail I. Bogachev (667390)   +9 more
core   +1 more source

Dactolisib outperforms Rapamycin in potentiating apoptosis induced by XPO1, Bcl‐2 and Mcl‐1 blockade in preclinical glioblastoma models

FEBS Open Bio, EarlyView.
The PAM pathway and its impact on cellular metabolism. Color‐coded key symbols represent Rapamycin (R, orange, targeting mTOR) and Dactolisib (D, green, targeting PI3K/mTOR), indicating their sites of action. Created with BioRenders.com. Glioblastoma resists therapy through interconnected survival programs.
Kai Zhao, Leonie Meyer, Madita Braun, Katharina Otte, Celine Bitter, Maite Pawlik, Hartmann Raifer, Frederik Helmprobst, Axel Pagenstecher, Hans Urban, Michael C. Burger, Michael W. Ronellenfitsch, Joachim P. Steinbach, Jarmila Jedelská, Shamim Bagheri, Behrooz H. Yousefi, Pietro Di Fazio, Markus Luster, Andreas H. Mahnken, Andreas Neubauer, Andreas Burchert, Christopher Nimsky, Jörg W. Bartsch, Tillmann Rusch   +23 more
wiley   +1 more source

Bacteriophage Interactions With Epithelial Cells: Therapeutic Implications

Frontiers in Microbiology, 2021
Andrzej Górski, Andrzej Górski, Andrzej Górski, Jan Borysowski, Ryszard Miȩdzybrodzki, Ryszard Miȩdzybrodzki, Ryszard Miȩdzybrodzki   +6 more
doaj   +1 more source

Prokaryotic microvesicles Ortholog of eukaryotic extracellular vesicles in biomedical fields

Cell Communication and Signaling
Every single cell can communicate with other cells in a paracrine manner via the production of nano-sized extracellular vesicles. This phenomenon is conserved between prokaryotic and eukaryotic cells.
Halimeh Mobarak, Farzin Javid, Maryam Taghavi Narmi, Narges Mardi, Fatemeh Sadeghsoltani, Parisa Khanicheragh, Samaneh Narimani, Mahdi Mahdipour, Emel Sokullu, Ferzane Valioglu, Reza Rahbarghazi   +10 more
doaj   +1 more source

Applications of Recombinant Dna Technology in Gastrointestinal Medicine and Hepatology: Basic Paradigms of Molecular Cell Biology. Part B: Eukaryotic Gene Transcription and Post-Transcripional Rna Processing

Canadian Journal of Gastroenterology, 2000
The transcription of DNA into RNA is the primary level at which gene expression is controlled in eukaryotic cells. Eukaryotic gene transcription  involves several different RNA polymerases that interact with a host of transcription factors to initiate ...
Gary E Wild, Patrizia Papalia, Mark J Ropeleski, Julio Faria, Alan BR Thomson   +4 more
doaj   +1 more source

Melanin's Role in Balancing Mechanical Strength and Reproductive Output in Environmentally Stressed Fungal Spores

Advanced Functional Materials, EarlyView.
Melanin content directly controls fungal spore mechanical properties, with pigmented spores achieving rupture forces two orders of magnitude higher than hyaline spores. Environmental stress drives this quantity‐quality tradeoff; stressed species invest energy in melanin‐reinforced, mechanically superior thick‐walled, super‐hydrophobic spores, while ...
Atul Agrawal, Steven E. Naleway
wiley   +1 more source