Results 111 to 120 of about 387,698 (305)

How phagocytic cells kill bacteria: Lessons from a professional killer

FEBS Open Bio, EarlyView.
How phagocytic cells ingest and kill bacteria has been studied for more than a century, but many questions remain unanswered. The study of the amoeba Dictyostelium discoideum brings new answers, and new questions. Professional phagocytic cells such as neutrophils and macrophages, as well as free‐living soil amoebae like Dictyostelium discoideum, employ
Otmane Lamrabet, Pierre Cosson
wiley   +1 more source

Stably Integrated for Assessment of Invasion Kinetics

Molecular Imaging, 2008
Salmonella Typhimurium is a common cause of gastroenteritis in humans and also localizes to neoplastic tumors in animals. Invasion of specific eukaryotic cells is a key mechanism of Salmonella interactions with host tissues.
Kelly N. Flentie, Min Qi, Seth T. Gammon, Yasmin Razia, Felix Lui, Luciano Marpegan, Aashish Manglik, David Piwnica-Worms, Jeffrey S. McKinney   +8 more
doaj   +1 more source

From energy provision to protein synthesis: Tunnelling nanotubes as mediators of intercellular metabolic cooperation in cancer

FEBS Open Bio, EarlyView.
The cytoskeleton‐mediated transport of mitochondria via tunnelling nanotubes restores respiration, increases ATP production, rescues cells from apoptosis, activates the AKT/mTOR signalling pathway, promotes cell migration and invasiveness, contributes to cancer progression and treatment resistance.
Stanislava Martínková, Jan Trnka
wiley   +1 more source

Interaction of yeast eIF4G with spliceosome components Implications in pre-mRNA processing events [PDF]

, 2009
As evidenced from mammalian cells the eukaryotic translation initiation factor eIF4G has a putative role in nuclear RNA metabolism. Here we investigate whether this role is conserved in the yeast Saccharomyces cerevisiae.
Lewis, Joe, Fromont-Racine, Micheline, Thompson, Elizabeth, Jacquier, Alain, Beggs, Jean, D., Barrass, J. David, Beggs, Jean D., Kafasla, Panagiota   +7 more
core   +1 more source

In silico and in vitro exploration of a tyrosinase for biocatalytic production of catechols

FEBS Open Bio, EarlyView.
Tyrosinase from Ralstonia pseudosolanacearum is a promising biocatalyst for producing valuable catechols from monophenol substrates. This tyrosinase is uniquely suited to this due to its high monophenolase : diphenolase ratio. We combined in silico docking and in vivo kinetic characterisation of this tyrosinase with 11 industrially relevant monophenols,
James Britton, Fang Zhao, Reeta Davis, Meg Walsh, Kevin E O'Connor, David O'Connell, Tanja Narancic   +6 more
wiley   +1 more source

Specialized Yeast Ribosomes: A Customized Tool for Selective mRNA Translation [PDF]

, 2013
Evidence is now accumulating that sub-populations of ribosomes - so-called specialized ribosomes - can favour the translation of subsets of mRNAs. Here we use a large collection of diploid yeast strains, each deficient in one or other copy of the set of ...
Klausegger, Alfred, Brandl Clemens, Lore Breitenbach-Koller, Clemens Br, Bjoern Wimmer (431586), Mick F, Wimmer, Bjoern, Johann W. Bauer (431583), Manuela Weber, Clemens Brandl, Tuite Mick F., Breitenbach-Koller, Hannelore, Klausegger Alfred, Manuela Weber (431587), Johann W. Bauer, Bauer Johann W., Breitenbach-Koller, Lore, Haubenreisser Olaf, Preiss, Thomas, Breitenbach-Koller Lore, Wimmer Bjoern, Helmut Hintner, Michael Breitenbach, Olaf Haubenreisser (431585), Hintner Helmut, Breitenbach, Michael, Mick F Tuite, Alfred Klausegger, Karl Thomas, Weber Manuela, Karl, Thomas, Hintner, Helmut, Bjoern Wimmer, Haar, Thomas Von der, Haubenreisser, Olaf, von der Haar Tobias, Clemens Brandl (431584), Bauer, Johann W., Johann W Bauer, Breitenbach Michael, Thomas Karl, Mick F. Tuite (266570), Lore Breitenbach-Koller (431591), Michael Breitenbach (46789), Bauer, Johann, Tuite, Mick F., von der Haar, Tobias, Olaf Haubenreisser, Helmut Hintner (431590), Brandl, Clemens, Tobias von der Haar, Tobias von der Haar (80575), Alfred Klausegger (431589), Thomas Karl (431588), Weber, Manuela   +54 more
core   +1 more source

Modification of bacterial effector proteins inside eukaryotic host cells

Frontiers in Cellular and Infection Microbiology, 2016
Pathogenic bacteria manipulate their hosts by delivering a number of virulence proteins -called effectors- directly into the plant or animal cells. Recent findings have shown that such effectors can suffer covalent modifications inside the eukaryotic ...
Crina Mihaela Popa, Crina Mihaela Popa, Mitsuaki Tabuchi, Marc Valls, Marc Valls   +4 more
doaj   +1 more source

Purification and preparation of Marchantia polymorpha Auxin Response Factor 2 for phase separation studies

FEBS Open Bio, EarlyView.
We describe detailed protocols for the purification and preparation of Marchantia polymorpha Auxin Response Factor 2 (MpARF2). This protein is fused to an MBP solubility tag and an mNG fluorescent tag and is purified from Escherichia coli. The presented procedures make it possible to study MpARF2 assemblies, which could arise from phase separation ...
Bas Janssen, Robin Romein, Willy A. M. van den Berg, Simon Lindhoud, Jan Willem Borst, Carlo P. M. van Mierlo   +5 more
wiley   +1 more source

Simultaneous Disruption of Two DNA Polymerases, Pol eta and Pol zeta, in Avian DT40 Cells Unmasks the Role of Pol eta in Cellular Response to Various DNA Lesions [PDF]

, 2010
Replicative DNA polymerases are frequently stalled by DNA lesions. The resulting replication blockage is released by homologous recombination (HR) and translesion DNA synthesis (TLS). TLS employs specialized TLS polymerases to bypass DNA lesions.
Sonoda, E, Dávid Szüts, Hirota Kouji, Takuo Kawamoto, Shunichi Takeda, Motegi, Akira, Motegi Akira, Fumio Hanaoka, Kawamoto Takuo, Julian E. Sale, Kawamoto, Takuo, Iwai, Shigenori, Julian E Sale, Sale Julian E., Sonoda Eiichiro, Masutani Chikahide, Shigenori Iwai, Alan Lehmann, Masutani, C, Takeda, Shunichi, Iwai Shigenori, Lehmann, Alan, Chikahide Masutani, Szüts, Dávid, Iwai, S, Eiichiro Sonoda, Motegi, A, Kawamoto, T, Masutani, Chikahide, Kouji Hirota, Hanaoka Fumio, Szüts, D, Takeda Shunichi, Hirota, Kouji, Sale, Julian E, Szüts Dávid, Hanaoka, Fumio, Lehmann Alan, Takeda, S, Hirota, K, Hanaoka, F, Sale, JE, Akira Motegi, Sonoda, Eiichiro, Lehmann, A   +44 more
core   +1 more source

Protocol for quantifying miRNA trafficking across the endosomal membrane

FEBS Open Bio, EarlyView.
An in vitro protocol measures miRNA uptake into endosomes isolated from mammalian cell extracts, which are free of subcellular contaminants. Performed at 37 °C in the presence of ATP, it ensures the import of single‐stranded miRNA into the endosomal lumen.
Syamantak Ghosh, Kamalika Mukherjee, Suvendra N. Bhattacharyya   +2 more
wiley   +1 more source