Results 91 to 100 of about 5,903,550 (301)

Conditional Glycosylation in Eukaryotic Cells Using a Biocompatible Chemical Inducer of Dimerization

, 2015
Conditional Glycosylation in Eukaryotic Cells Using a Biocompatible Chemical Inducer of ...
Jennifer L. Czlapinski (1265382), Jennifer J. Kohler (234258), Scott T. Laughlin (1265388), Michael W. Schelle (418896), Carolyn R. Bertozzi (187405), Lawrence W. Miller (1265391), Virginia W. Cornish (1265385)   +6 more
core   +1 more source

Versatile vector tools for efficient protein screening across multiple expression systems

FEBS Open Bio, EarlyView.
A unified vector toolkit enables rapid protein expression screening across E. coli, insect, and mammalian cells. A single primer pair amplifies the target gene, which is inserted into any vector via a standardized interface. This streamlined workflow eliminates repeated cloning steps, accelerating the identification of optimal expression conditions for
Zhimin Zhu, Yaqing Liu, Lei Qin, Feng Yu, Qisheng Wang, Yahui Liu   +5 more
wiley   +1 more source

Editorial: Structural aspects of peroxisome biogenesis and functions

Frontiers in Cell and Developmental Biology, 2022
Marek Skoneczny, Tânia Francisco, Tânia Francisco, Ralf Erdmann, Christos Gatsogiannis   +4 more
doaj   +1 more source

Directed evolution of enzymes at the crossroads of tradition and innovation

FEBS Open Bio, EarlyView.
An iterative cycle of data‐driven enzyme optimization comprising four stages: genetic diversification of a template enzyme, expression of protein variants, high‐throughput evaluation, and machine‐learning‐guided redesign of the next variant library.
Maria Tomkova, Andrej Mirossay, Erik Sedlak   +2 more
wiley   +1 more source

Printing of eukaryotic cells using screening platform.

, 2015
A plate was printed with yeast from the species Pichia pastoris in 3066 matrix format onto an YPD agar charcoal plate using the Fluorescence Activated Cell Sorter, showing the proteins carried in eukaryotic cells as well as prokaryotic cells could be put
Martin Pule (821995), Arnold Pizzey (258949), Mark Lythgoe (821994), Cassandra Stowe (821992), Tammy Kalber (821993), Adam Badar (353606)   +5 more
core   +1 more source

Small RNA pathways in mammalian oocytes

FEBS Open Bio, EarlyView.
Three distinct small RNA pathways operate in mammalian oocytes: RNAi interference (RNAi), the microRNA (miRNA) pathway, and the PIWI‐associated RNA (piRNA) pathway. These pathways use small RNAs to guide sequence‐specific repression and contribute to oocyte biology by targeting genes and mobile elements or appear insignificant since different ...
Petr Svoboda, Josef Pasulka
wiley   +1 more source

Transcription factor clusters regulate genes in eukaryotic cells

eLife, 2017
Transcription is regulated through binding factors to gene promoters to activate or repress expression, however, the mechanisms by which factors find targets remain unclear.
Adam JM Wollman, Sviatlana Shashkova, Erik G Hedlund, Rosmarie Friemann, Stefan Hohmann, Mark C Leake   +5 more
doaj   +1 more source

Hyperactive ice‐binding proteins stabilize cell membranes and improve resistance to dehydration stress in Caenorhabditis elegans

FEBS Open Bio, EarlyView.
TisIBP8, a fungal‐derived hyperactive ice‐binding protein, helps Caenorhabditis elegans survive dehydration. It localizes near cell membranes, reduces cell damage, and helps maintain membrane structure during drying. These results suggest that ice‐binding proteins can protect cells from dehydration stress as well as freezing stress.
Daiki Shimose, Kotaro Ozaki, Ryohei Kuriyama, Yuka Ikemoto, Kazuhiro Mio, Yuji C. Sasaki, Tatsuya Arai, Sakae Tsuda, Yoichi Shinkai, Masahiro Kuramochi   +9 more
wiley   +1 more source

Pivotal role of the non-hr origin of DNA replication in the genesis of defective interfering baculoviruses [PDF]

, 2002
The generation of deletion mutants, including defective interfering viruses, upon serial passage of Spodoptera exigua multicapsid nucleopolyhedrovirus (SeMNPV) in insect cell culture has been studied. Sequences containing the non-homologous region origin
Pijlman, G.P., Yang, K., Martens, D.E., Goldbach, R.W., Vlak, J.M., Dortmans, Jos C. F. M., Vermeesch, Angela M. G., Goldbach, Rob W., Martens, Dirk E., Pijlman, Gorben P., Yang, Kai, Vlak, Just M., Dortmans, J.C.F.M., Vermeesch, A.M.G.   +13 more
core   +1 more source

DNA mismatch repair and its many roles in eukaryotic cells

, 2017
DNA mismatch repair (MMR) is an important DNA repair pathway that plays critical roles in DNA replication fidelity, mutation avoidance and genome stability, all of which contribute significantly to the viability of cells and organisms. MMR is widely-used
Keijzers, Guido, Dekang Liu, Guido Keijzers, Liu, Dekang, Lene Juel Rasmussen, Rasmussen, Lene Juel   +5 more
core   +2 more sources