Results 71 to 80 of about 5,903,550 (301)

Timing the origin of eukaryotic cellular complexity with ancient duplications [PDF]

, 2020
Eukaryogenesis is one of the most enigmatic evolutionary transitions, during which simple prokaryotic cells gave rise to complex eukaryotic cells. While evolutionary intermediates are lacking, gene duplications provide information on the order of events ...
Marcet-Houben, Marina, Gabaldón, Toni, Vlimmeren, Anne van, van Wijk, Leny, van Mijk, Leny M., Wijk, Leny M. van, Snel, Berend, Marcet Houben, Marina, van Vlimmeren, Anne, Vosseberg, Julian, van Hooff, Jolien J.E., Gabaldón Estevan, Juan Antonio, 1973-, van Hooff, Jolien, Hooff, Jolien J. E. van   +13 more
core   +1 more source

Invisible but not inaccessible—Revealing transient oligomers formed by intrinsically disordered proteins with solution NMR and complementary methods

FEBS Letters, EarlyView.
Transient oligomers formed by intrinsically disordered proteins may be ‘invisible’ to direct detection yet remain accessible to solution NMR through equilibrium‐exchange measurements and pressure‐jump experiments. Complementary methods report on mass, stoichiometry, selected distance distributions, morphology, and internal packing.
Martin D. Gelenter, Ad Bax
wiley   +1 more source

Prokaryotic and Eukaryotic Cells (6th grade) [PDF]

, 2016
This unit is meant to provide a concrete and hands-on approach to introducing cells and differences between prokaryotic cells (cells without a nucleus), and eukaryotic cells (cells with a nucleus).
Bos, Samantha
core   +1 more source

Going viral − Virus‐targeting nanobodies for research, diagnostics and therapy

FEBS Letters, EarlyView.
The increasing prevalence of viral diseases represents a major threat for global human health. Nanobodies, small binding fragments derived from camelid heavy‐chain antibodies, offer versatile tools for viral research, diagnostics, and antiviral therapy.
Domenic Ebert, Ulrich Rothbauer
wiley   +1 more source

Intrabody expression in eukaryotic cells.

, 2009
International audienceWe describe procedures for intracellular expression of scFv in eukaryotic cells. Starting from a scFv gene cloned in a phage-display vector, we describe the cloning step into a mammalian expression vector, the transient transfection
Martineau, Pierre, Laurence Guglielmi, Pierre Martineau, Guglielmi, Laurence   +3 more
core   +1 more source

A small molecule inhibitor of leucine carboxyl methyltransferase-1 inhibits cancer cell survival

Frontiers in Drug Discovery
Reversible phosphorylation is the basis for signal transduction in eukaryotic cells, and this is tightly controlled by the complex interplay of kinases and phosphatases.
O. A. Arosarena, O. A. Arosarena, O. A. Arosarena, A. S. Saribas, E. P. Papadopoulos   +4 more
doaj   +1 more source

An Autocrine Proliferation Repressor Regulates Dictyostelium discoideum Proliferation and Chemorepulsion Using the G Protein-Coupled Receptor GrlH

mBio, 2018
In eukaryotic microbes, little is known about signals that inhibit the proliferation of the cells that secrete the signal, and little is known about signals (chemorepellents) that cause cells to move away from the source of the signal.
Yu Tang, Yuantai Wu, Sarah E. Herlihy, Francisco J. Brito-Aleman, Jose H. Ting, Chris Janetopoulos, Richard H. Gomer   +6 more
doaj   +1 more source

Soft motions encoded by the fold and mediated by conserved hinge sites drive V‐ATPase allosteric machinery and autoinhibition

FEBS Letters, EarlyView.
Vacuolar H+‐ATPase (V‐ATPase) is a multisubunit rotary proton pump that acidifies intracellular compartments. Using elastic network models, we identified conserved hinge residues that govern its large‐scale functional motions. We show that intrinsically encoded ‘soft modes’ drive critical functional transitions, including rotary catalysis and ...
Diksha Parwana, Xiaowei Bogetti, Stuti Sharma, Ivet Bahar   +3 more
wiley   +1 more source

The symbiotic origin of the eukaryotic cell

Comptes Rendus. Biologies, 2023
Eukaryogenesis represented a major evolutionary transition that led to the emergence of complex cells from simpler ancestors. For several decades, the most accepted scenario involved the evolution of an independent lineage of proto-eukaryotes endowed with an endomembrane system, including a nuclear compartment, a developed cytoskeleton and phagocytosis,
Purificación López-García, David Moreira   +1 more
openaire   +4 more sources

How structural disorder and polyelectrolyte properties of prothymosin α enable its large interactome

FEBS Letters, EarlyView.
In this review, complexes of the fully disordered and highly negatively charged prothymosin α are collected, described, and discussed. Linked to its poly‐electrostatic character, prothymosin α engages a large interactome through multiple different binding modes ranging from fully disordered complexes to short linear motif (SLiM)‐driven interactions ...
Ketty C. Tamburrini, Freia S. Buus, Benjamin Schuler, Birthe B. Kragelund   +3 more
wiley   +1 more source