Results 11 to 20 of about 11,020,597 (230)

Engineered transcription-associated Cas9 targeting in eukaryotic cells [PDF]

Nature Communications
DNA targeting Class 2 CRISPR-Cas effector nucleases, including the well-studied Cas9 proteins, evolved protospacer-adjacent motif (PAM) and guide RNA interactions that sequentially license their binding and cleavage activities at protospacer target sites.
Gregory W. Goldberg, Manjunatha Kogenaru, Sarah Keegan, Max A. B. Haase, Larisa Kagermazova, Mauricio A. Arias, Kenenna Onyebeke, Samantha Adams, Daniel K. Beyer, David Fenyö, Marcus B. Noyes, Jef D. Boeke   +11 more
doaj   +2 more sources

What happens to Bifidobacterium adolescentis and Bifidobacterium longum ssp. longum in an experimental environment with eukaryotic cells? [PDF]

BMC Microbiology
Background The impact of probiotic strains on host health is widely known. The available studies on the interaction between bacteria and the host are focused on the changes induced by bacteria in the host mainly.
Dominika Jakubczyk, Katarzyna Leszczyńska, Katarzyna Pacyga-Prus, Dominika Kozakiewicz, Wioletta Kazana-Płuszka, Dominika Gełej, Paweł Migdał, Roksana Kruszakin, Agnieszka Zabłocka, Sabina Górska   +9 more
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Bioenergetic myths of energy transduction in eukaryotic cells [PDF]

Frontiers in Molecular Biosciences
The study of energy transduction in eukaryotic cells has been divided between Bioenergetics and Physiology, reflecting and contributing to a variety of Bioenergetic myths considered here: 1) ATP production = energy production, 2) energy transduction is ...
Guy C. Brown
doaj   +2 more sources

Interactions between Bacteriophages and Eukaryotic Cells [PDF]

Scientifica, 2020
As the name implies, bacteriophage is a bacterium-specific virus. It infects and kills the bacterial host. Bacteriophages have gained attention as alternative antimicrobial entities in the science community in the western world since the alarming rise of
Ramendra Dirgantara Putra, Diana Lyrawati   +1 more
doaj   +2 more sources

Applications of Recombinant Dna Technology in Gastrointestinal Medicine and Hepatology: Basic Paradigms of Molecular Cell Biology. Part B: Eukaryotic Gene Transcription and Post-Transcripional Rna Processing [PDF]

Canadian Journal of Gastroenterology, 2000
The transcription of DNA into RNA is the primary level at which gene expression is controlled in eukaryotic cells. Eukaryotic gene transcription  involves several different RNA polymerases that interact with a host of transcription factors to initiate ...
Gary E Wild, Patrizia Papalia, Mark J Ropeleski, Julio Faria, Alan BR Thomson   +4 more
doaj   +2 more sources

Successful Expression of DNA-Based Vaccine Construct Encoding Human Papillomavirus Type 16 E7 Fused to Heat Shock Protein B1 in Eukaryotic Cells

Journal of Medical Microbiology and Infectious Diseases, 2023
Introduction: Developing potent therapeutic vaccines against human papillomaviruses (HPVs) is crucial for the effective management of various HPV-associated cancers.
Reyhane Najafi1, Azam Bolhassani2*, Maryam Montazeri1, Elnaz Agi3   +3 more
doaj   +1 more source

Structure and function of bacterial dynamin-like proteins [PDF]

, 2012
Membrane dynamics are essential for numerous cellular processes in eukaryotic and prokaryotic cells. In eukaryotic cells, membrane fusion and fission are often catalyzed by large GTPases of the dynamin protein family. These proteins couple GTP hydrolysis
Bramkamp, Marc
core   +1 more source

Isolation of Myricitrin and 3,5-di-O-Methyl Gossypetin from Syzygium samarangense and Evaluation of their Involvement in Protecting Keratinocytes against Oxidative Stress via Activation of the Nrf-2 Pathway

Molecules, 2019
The wax apple (Syzygium samarangense) is traditionally employed as an antibacterial and immunostimulant drug in traditional medicine. This plant is rich in different flavonoids and tannins. In this study, we isolated two compounds from S.
Mansour Sobeh, Ganna Petruk, Samir Osman, Mohamed A. El Raey, Paola Imbimbo, Daria Maria Monti, Michael Wink   +6 more
doaj   +1 more source

Quantification of DNA-associated proteins inside eukaryotic cells using single-molecule localization microscopy [PDF]

, 2014
Development of single-molecule localization microscopy techniques has allowed nanometre scale localization accuracy inside cells, permitting the resolution of ultra-fine cell structure and the elucidation of crucial molecular mechanisms.
Adam T. Watson, Alex Herbert, Antony M. Carr, Betzig, Bähler, Culbertson, David Klenerman, David Lando, Dion, Elf, Ernest Laue, Forsburg, Heilemann, Henteges, Hess, Horrocks, Jem Tucker, Kearsey, Kim, Krichevsky, Kubota, Lando, Manley, Mark A. Osborne, Masai, Matsuyama, Matthieu Palayret, Meister, Michalet, Mortensen, Patterson, Peter Jönsson, Reyes-Lamothe, Rust, Rémi L. Boulineau, Shiomi, Sophie George, Spendier, Steven F. Lee, Stracy, Su'etsugu, Szymborska, Thomas J. Etheridge, Tokunaga, Uphoff, Watson, Yasukazu Daigaku   +46 more
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Engineering circular RNA for potent and stable translation in eukaryotic cells

Nature Communications, 2018
Messenger RNA (mRNA) has broad potential for application in biological systems. However, one fundamental limitation to its use is its relatively short half-life in biological systems.
A. Wesselhoeft, Piotr S. Kowalski, Daniel G. Anderson   +2 more
semanticscholar   +1 more source