Results 101 to 110 of about 204,342 (301)
A common mechanism for recruiting the Rrm3 and RTEL1 accessory helicases to the eukaryotic replisome
The EMBO JournalThe eukaryotic replisome is assembled around the CMG (CDC45-MCM-GINS) replicative helicase, which encircles the leading-strand DNA template at replication forks.
Ottavia Olson +3 more
doaj +1 more source
Replication Stress and Consequential Instability of the Genome and Epigenome
Molecules, 2019 Cells must faithfully duplicate their DNA in the genome to pass their genetic information to the daughter cells. To maintain genomic stability and integrity, double-strand DNA has to be replicated in a strictly regulated manner, ensuring the accuracy of ...
Pawlos S. Tsegay, Yanhao Lai, Yuan Liu
doaj +1 more source
Embedded CRISPRi Enhances Gene‐Silencing Efficiency in Drosophila
Advanced Science, EarlyView.Current CRISPR interference (CRISPRi) technology in Drosophila has limited efficiency. This study introduces the emCRISPRi platform, which significantly enhances transcriptional silencing efficacy by embedding inhibitory domains within the dCas9 architecture.
Pengchong Fu +7 more
wiley +1 more source
Monitoring and quantifying replication fork dynamics with high-throughput methods
Communications BiologyBefore each cell division, eukaryotic cells must replicate their chromosomes to ensure the accurate transmission of genetic information. Chromosome replication involves more than just DNA duplication; it also includes chromatin assembly, inheritance of ...
Nora Fajri, Nataliya Petryk
doaj +1 more source
Two subunits of human ORC are dispensable for DNA replication and proliferation
eLife, 2016 The six-subunit Origin Recognition Complex (ORC) is believed to be an essential eukaryotic ATPase that binds to origins of replication as a ring-shaped heterohexamer to load MCM2-7 and initiate DNA replication. We have discovered that human cell lines in
Etsuko Shibata +5 more
doaj +1 more source
Distinct RPA functions promote eukaryotic DNA replication initiation and elongation. [PDF]
Nucleic Acids Res, 2023 Pike AM, Friend CM, Bell SP.
europepmc +1 more source
Transcription as a Threat to Genome Integrity [PDF]
, 2016 Genomes undergo different types of sporadic alterations, including DNA damage, point mutations, and genome rearrangements, that constitute the basis for evolution.
Aguilera López, Andrés +1 more
core +1 more source
Alternating High‐Fat and Polysaccharide Diets Modulates Gut Phage‐Bacterial Interplay
Advanced Science, EarlyView.This study reveals how alternating high‐fat and polysaccharide diets reshape the human gut virome and enhance phage‐bacteria interactions. Using large‐scale metagenomic meta‐analysis and a time‐resolved mouse model, the authors show that diets strongly modulate phage abundance, lifestyle, and gene exchange, offering new insights into nutrition‐guided ...
Fengxiang Zhao +6 more
wiley +1 more source
Advanced Science, EarlyView.This study performs pan‐viromic profiling of 14,529 samples from 5,710 domestic herbivores across five Chinese provinces, establishing the DhCN‐Virome (1,085,360 viral metagenomes). It reveals species/sample‐specific viromic signatures and cross‐species transmission dynamics, aiding unified disease control.
Yue Sun +19 more
wiley +1 more source
Replisome Dynamics and Their Functional Relevance upon DNA Damage through the PCNA Interactome
Cell Reports, 2018 Summary: Eukaryotic cells use copious measures to ensure accurate duplication of the genome. Various genotoxic agents pose threats to the ongoing replication fork that, if not efficiently dealt with, can result in replication fork collapse. It is unknown
Mrinal Srivastava +6 more
doaj +1 more source