Results 81 to 90 of about 204,342 (301)

Cell Cycle Control of Nuclear Metabolism Couples Phosphatidylinositol Signaling to Histone Methylation

Advanced Science, EarlyView.
Nuclear metabolism oscillates during cell cycle progression. Quantitative chromatome proteomics and imaging reveal phase‐specific dynamics of PIP5K1A and nuclear PIP2, linking phosphatidylinositol metabolism to histone methylation. This work identifies nuclear lipid metabolism as a previously unrecognized regulatory axis coordinating chromatin ...
Antoni Gañez‐Zapater, Savvas Kourtis, Camilla Reiter Elbæk, Lorena Espinar, Carolina Toro‐Márquez, Albert Coll‐Manzano, Alfredo Smiriglia, Laura García‐López, Laura Wiegand, Maria Guirola, Frédéric Fontaine, Andrea Morandi, André C. Müller, Sara Sdelci   +13 more
wiley   +1 more source

Mechanistic models of asymmetric hand-over-hand translocation and nucleosome navigation by CMG helicase

Nature Communications
Faithful replication of eukaryotic chromatin requires the CMG helicase to translocate directionally along single-stranded DNA (ssDNA) while unwinding double-stranded DNA (dsDNA) and navigating nucleosomes.
Fritz Nagae, Yutaka Murata, Masataka Yamauchi, Shoji Takada, Tsuyoshi Terakawa   +4 more
doaj   +1 more source

Structure and Function of a Mycobacterial NHEJ DNA Repair Polymerase [PDF]

, 2006
Non homologous end-joining (NHEJ)-mediated repair of DNA double-strand breaks in prokaryotes requires Ku and a specific multidomain DNA ligase (LigD).
Aidan J. Doherty, Angel J. Picher, Arezi, Augustin, Bowater, Chen, Chen, Critchlow, Daley, Darren Thompson, DeFazio, Della, Dominguez, Emsley, Garcia-Diaz, Gavin C. Fox, Gong, Gong, Ito, Iyer, Jones, Krejci, La Fortelle, Lao-Sirieix, Lao-Sirieix, Lipps, Luis Blanco, Ma, Ma, Mahajan, Matsumoto, McCoy, Murshudov, Nick McElhinney, Nick McElhinny, Nick McElhinny, Nigel C. Brissett, Paula Andrade, Paull, Picher, Pitcher, Pitcher, Potterton, Potterton, Powell, Prasad, Ramsden, Raquel Juarez, Robert S. Pitcher, Ruiz, Rydberg, Singhal, Steitz, Tseng, Weller, Wilson, Winn, Wu, Zhang, Zhu   +59 more
core   +1 more source

A Bifunctional T3SS‐Effector Simultaneously Cleaves Host MAP Kinase and Inhibits PPM1A Phosphatase

Advanced Science, EarlyView.
Pathogenic bacteria exploit the metalloprotease effector NleD to subvert host defenses. Structural, biochemical, and infection analyses reveal a bifunctional mechanism by which NleD binds and inhibits the host phosphatase PPM1A while preserving its proteolytic activity against MAPKs.
Yaakov Socol, Lihi Gur‐Arie, Netanel Tzarum, Tamara Wellins, Naama Katsowich, Miriam Ravins, Michal Bejerano‐Sagie, Klil Cohen, Oded Livnah, Joshua N. Adkins, Ernesto Nakayasu, Alexei Savchenko, Yeu Khai Choong, Nikhil Kumar Tulsian, Saturo Machida, Sigal Ben‐Yehuda, Yael Litvak, J. Sivaraman, Ilan Rosenshine   +18 more
wiley   +1 more source

Detection of Protein–Protein Interactions in Escherichia coli With Single Molecule Sensitivity

Advanced Science, EarlyView.
This article describes a regulatory circuit in Escherichia coli able to detect protein–protein interactions with exquisite sensitivity. The interaction between two hybrid proteins fused to Bordetella pertussis adenylate cyclase and its activator calmodulin triggers a potent cyclic AMP signaling cascade.
Marilyne Davi, Daniel Ladant
wiley   +1 more source

The major roles of DNA polymerases epsilon and delta at the eukaryotic replication fork are evolutionarily conserved.

PLoS Genetics, 2011
Coordinated replication of eukaryotic genomes is intrinsically asymmetric, with continuous leading strand synthesis preceding discontinuous lagging strand synthesis.
Izumi Miyabe, Thomas A Kunkel, Antony M Carr   +2 more
doaj   +1 more source

Role of the ubiquitin-selective CDC-48/UFD-1/NPL-4 chaperone in DNA replication [PDF]

, 2012
Faithful transmission of genomic information requires tight spatiotemporal regulation of DNA replication factors. Posttranslational modifications, such as ubiquitylation, constitute a fast and effective mechanism to control such complex protein function.
Franz, André
core  

Fork rotation and DNA precatenation are restricted during DNA replication to prevent chromosomal instability [PDF]

, 2015
Faithful genome duplication and inheritance require the complete resolution of all intertwines within the parental DNA duplex. This is achieved by topoisomerase action ahead of the replication fork or by fork rotation and subsequent resolution of the DNA
Anna L. Chambers, Hiasa, Jessica A. Downs, Jonathan Baxter, Sahar Mansoubi, Stephanie A. Schalbetter   +5 more
core   +3 more sources

Telomere‐to‐Telomere Genomes Reveal that Multiscale Evolution Shapes the Largest Metabolic Arsenal of Diaporthe Fungi

Advanced Science, EarlyView.
This study presents the first telomere‐to‐telomere genomes and population resources for Diaporthe pathogens, uncovering the largest known fungal repertoire of secondary metabolite gene clusters. Structural variations and horizontal gene transfer drive cluster diversification, while specific rapidly evolving clusters control virulence, offering novel ...
Kainan Li, Chen Zhang, Zhichao Zhang, Jiaheng Cheng, Qiuyan Gao, Long Gao, Xiaolin Zhao, Wei Yan, Yuanchao Wang, Wenwu Ye   +9 more
wiley   +1 more source

pFAR plasmids: New Eukaryotic Expression Vectors for Gene Therapy, devoid of Antibiotic Resistance Markers [PDF]

, 2008
Efficient production of eukaryotic expression vectors requires the selection of plasmid-containing bacteria. To avoid the risk of dissemination of antibiotic resistance markers, we developed a new system to produce a family of plasmids Free of Antibiotic
Corinne Marie, Daniel Scherman, Gaelle Vandermeulen, Magali Richard, Mickael Quiviger, Veronique Preat   +5 more
core   +1 more source