Results 61 to 70 of about 204,342 (301)

Minichromosome Maintenance Proteins: From DNA Replication to the DNA Damage Response

Cells
The DNA replication machinery is highly conserved from bacteria to eukaryotic cells. Faithful DNA replication is vital for cells to transmit accurate genetic information to the next generation. However, both internal and external DNA damages threaten the
Agnes Malysa, Xiaohong Mary Zhang, Gerold Bepler   +2 more
doaj   +1 more source

Enzymes involved in organellar DNA replication in photosynthetic eukaryotes

Frontiers in Plant Science, 2014
Plastids and mitochondria possess their own genomes. Although the replication mechanisms of these organellar genomes remain unclear in photosynthetic eukaryotes, several organelle-localized enzymes related to genome replication, including DNA polymerase,
Takashi eMoriyama, Takashi eMoriyama, Naoki eSato, Naoki eSato   +3 more
doaj   +1 more source

MultiBac: expanding the research toolbox for multiprotein complexes [PDF]

, 2012
This article is made available for unrestricted research re-use and secondary analysis in any form or by any means with acknowledgement of the original source.
Berger, Imre, Bieniossek, Christoph, Imasaki, Tsuyoshi, Takagi, Yuichiro   +3 more
core   +1 more source

Screening and epitope characterization of Nidogen‐2‐specific nanobodies

FEBS Open Bio, EarlyView.
Camel immunization and phage display were employed to generate high‐affinity VHH nanobodies against Nidogen‐2. After library construction, biopanning, ELISA screening, sequencing, and recombinant expression, selected nanobodies were purified and characterized, leading to the preliminary exploration of a nanobody‐based sandwich ELISA for specific ...
Jianchuan Wen, Qianqian Cui, Zhongyun Lan, Yingjun Wang, Shuaiying Zhao, Wenxuan Feng, Yunfeng Liu, Qiting Huang, Dongna Zhang, Jianfeng Xu   +9 more
wiley   +1 more source

Eukaryotic Replisome Components Cooperate to Process Histones During Chromosome Replication

Cell Reports, 2013
DNA unwinding at eukaryotic replication forks displaces parental histones, which must be redeposited onto nascent DNA in order to preserve chromatin structure.
Magdalena Foltman, Cecile Evrin, Giacomo De Piccoli, Richard C. Jones, Rick D. Edmondson, Yuki Katou, Ryuichiro Nakato, Katsuhiko Shirahige, Karim Labib   +8 more
doaj   +1 more source

Pharmacological inhibition of the PERK pathway modulates hepatocellular carcinoma growth and immune signaling

FEBS Open Bio, EarlyView.
Pharmacological inhibition of PERK in a DEN‐induced mouse model of liver cancer does not reduce tumor burden but alters cellular stress signaling. Despite blocking PERK activity, downstream stress responses, including CHOP expression, remain active, suggesting compensatory mechanisms within the unfolded protein response that may influence tumor ...
Ada Lerma‐Clavero, Maria Kopsida, Nathalie Arendt, Hans Lennernäs, Markus Sjöblom, Femke Heindryckx   +5 more
wiley   +1 more source

Regulated Transport into the Nucleus of Herpesviridae DNA Replication Core Proteins

Viruses, 2013
The Herpesvirdae family comprises several major human pathogens belonging to three distinct subfamilies. Their double stranded DNA genome is replicated in the nuclei of infected cells by a number of host and viral products.
Alessandro Ripalti, Arianna Loregian, Simone Avanzi, Daria Camozzi, David A. Jans, Alvisi Gualtiero, Giorgio Palù   +6 more
doaj   +1 more source

Amyloidogenic Peptide Fragments Designed From Bacterial Collagen‐like Proteins Form Hydrogel

Advanced Functional Materials, EarlyView.
This study identified amyloidogenic sequence motifs in bacterial collagen‐like proteins and exploited these to design peptides that self‐assemble into β‐sheet fibers and form hydrogels. One hydrogel supported healthy fibroblast growth, showing promise for biocompatible materials. Our work demonstrates that bacterial sequences can be harnessed to create
Vamika Sagar, Xuen J Ng, Lukas Lechel, Ashish Chand Rajwar, Hendrik Bargel, Abhishek A. Jalan   +5 more
wiley   +1 more source

The small subunit of DNA polymerase D (DP1) associates with GINS–GAN complex of the thermophilic archaea in Thermococcus sp. 4557

MicrobiologyOpen, 2019
The eukaryotic GINS, Cdc45, and minichromosome maintenance proteins form an essential complex that moves with the DNA replication fork. The GINS protein complex has also been reported to associate with DNA polymerase. In archaea, the third domain of life,
Shuhong Lu, Xuesong Zhang, Kaiying Chen, Zimeng Chen, Yixiang Li, Zhongquan Qi, Yulong Shen, Zhuo Li   +7 more
doaj   +1 more source

Human PrimPol mutation associated with high myopia has a DNA replication defect [PDF]

, 2014
PrimPol is a primase-polymerase found in humans, and other eukaryotes, involved in bypassing lesions encountered during DNA replication. PrimPol employs both translesion synthesis and repriming mechanisms to facilitate lesion bypass by the replisome ...
Aguilera, Aidan J. Doherty, Benjamin A. Keen, Bianchi, Biertümpfel, Brissett, Brown, Donigan, Garcia-Gomez, Iyer, Jones, Keen, Laura J. Bailey, Li, Lobley, Morgan, Mourón, Nemec, Nemec, Pitcher, Rudd, Sale, Saw, Stanislaw K. Jozwiakowski, Vitale, Wan, Washington, Zhao   +27 more
core   +1 more source