Results 71 to 80 of about 204,342 (301)

SiO2‐CaOCME/Poly(Tetrahydrofuran)/Poly(Caprolactone) 3D‐Printed Scaffolds Drive Human‐Bone Marrow Stromal Cell Osteogenic Differentiation

Advanced Healthcare Materials, EarlyView.
3D printed hybrid scaffolds combining bioactive silica–calcium chemistry with elastic polymers guide human bone stem cells to form bone. The scaffolds support cell survival, organization, and invasion while releasing osteogenic ions. Together, architecture and composition drive bone‐specific gene expression, extracellular matrix organization, and ...
David R. Sory, Agathe C.M. Heyraud, Julian R. Jones, Sara M. Rankin   +3 more
wiley   +1 more source

Improved proteomic analysis of nuclear proteins, as exemplified by the comparison of two myelo\"id cell lines nuclear proteomes [PDF]

, 2012
One of the challenges of the proteomic analysis by 2D-gel is to visualize the low abundance proteins, particularly those localized in organelles. An additional problem with nuclear proteins lies in their strong interaction with nuclear acids.
Chevallet, Mireille, Diemer, Hélène, Lelong, Cécile, Luche, Sylvie, Rabilloud, Thierry, Van Dorsselaer, Alain   +5 more
core   +4 more sources

Dual‐Peptide Nanoplatform: Mesoporous Silica Nanoparticles Functionalized With a Cell‐Penetrating Peptide and Loaded With Rationally Designed Antimicrobial Peptides for Tuberculosis Therapy

Advanced Healthcare Materials, EarlyView.
Machine learning–guided engineering of a plectasin‐derived peptide yields DC05, a potent antimycobacterial candidate. Encapsulation into tuftsin‐functionalized mesoporous silica nanoparticles enhances intracellular delivery, stability, and activity against Mycobacterium tuberculosis while maintaining low cytotoxicity and minimal hemolysis. The combined
Christian S. Carnero Canales, Cesar Augusto Roque‐Borda, Ana Carolina Cerqueira Negri, Oswaldo Ramirez Delgado, Letícia Oliveira Catarin Nunes, Flávia Aparecida Resende, Hernane Barud, Norival Alves Santos‐Filho, Saulo Santesso Garrido, Karen Cristina Oliveira, Alexandra Ivo de Medeiros, Rafael Miguel Sábio, Fernando Rogério Pavan   +12 more
wiley   +1 more source

In vitro Assays for Eukaryotic Leading/Lagging Strand DNA Replication

Bio-Protocol, 2017
The eukaryotic replisome is a multiprotein complex that duplicates DNA. The replisome is sculpted to couple continuous leading strand synthesis with discontinuous lagging strand synthesis, primarily carried out by DNA polymerases ε and δ, respectively ...
Grant Schauer, Jeff Finkelstein, Mike O’Donnell   +2 more
doaj   +1 more source

DNA unwinding mechanism of a eukaryotic replicative CMG helicase

Nature Communications, 2020
The DNA duplex is known to be split apart in a steric exclusion manner during replication, but the specific mechanism has remained unclear. Here the authors present a cryo-EM structure of a eukaryotic replicative CMG helicase on forked DNA, revealing the
Zuanning Yuan, Roxana Georgescu, Lin Bai, Dan Zhang, Huilin Li, Michael E. O’Donnell   +5 more
doaj   +1 more source

An archaeal family-B DNA polymerase variant able to replicate past DNA damage: occurrence of replicative and translesion synthesis polymerases within the B family [PDF]

, 2014
A mutant of the high fidelity family-B DNA polymerase from the archaeon Thermococcus gorgonarius (Tgo-Pol), able to replicate past DNA lesions, is described.
Aidan J. Doherty, Barry, Bebenek, Bernard A. Connolly, Biles, Boiteux, Braithwaite, Brautigam, Brian J. Keith, Burgers, Cadet, Cann, Cann, Cline, Cozens, Cubonová, Fenton, Firbank, Franklin, Gan, Goodman, Haracska, Haracska, Heller, Hogg, Ishino, Johnson, Johnson, Johnson, Joyce, Jozwiakowski, Jozwiakowski, Keith, Kelley, Kelman, Kim, Kunkel, Kunkel, Lawrence, Lawrence, Lawrence, Leadon, Leigh, Louise Gilroy, Lundberg, McCulloch, Nick McElhinny, Northam, Perera, Pettersen, Pfeifer, Prakash, Pursell, Rangarajan, Reha-Krantz, Sabouri, Sabouri, Sarmiento, Schneider, Shaw, Stanislaw K. Jozwiakowski, Stillman, Swan, Teoule, Terpe, Wang, Wang, Waters, Yang   +68 more
core   +1 more source

Bacteria‐Responsive Nanostructured Drug Delivery Systems for Targeted Antimicrobial Therapy

Advanced Materials, EarlyView.
Bacteria‐responsive nanocarriers are designed to release antimicrobials only in the presence of infection‐specific cues. This selective activation ensures drug release precisely at the site of infection, avoiding premature or indiscriminate release, and enhancing efficacy.
Guillermo Landa, Gracia Mendoza, Silvia Irusta, Manuel Arruebo   +3 more
wiley   +1 more source

Forks on the Run: Can the Stalling of DNA Replication Promote Epigenetic Changes?

Frontiers in Genetics, 2017
Built of DNA polymerases and multiple associated factors, the replication fork steadily progresses along the DNA template and faithfully replicates DNA. This model can be found in practically every textbook of genetics, with the more complex situation of
Hollie Rowlands, Piriththiv Dhavarasa, Ashley Cheng, Krassimir Yankulov   +3 more
doaj   +1 more source

MASQC: Next Generation Sequencing Assists Third Generation Sequencing for Quality Control in N6-Methyladenine DNA Identification

Frontiers in Genetics, 2020
DNA N6-methyladenine (6mA) modification has been discovered as the most prevalent DNA modification in prokaryotes and eukaryotes, involving gene expression, DNA replication and repair, and host-pathogen interactions. Single-molecule real-time sequencing (
Siqian Yang, Yaoxin Wang, Ying Chen, Qi Dai   +3 more
doaj   +1 more source

Molecular Evolution and Functional Diversification of Replication Protein A1 in Plants [PDF]

, 2016
Replication protein A (RPA) is a heterotrimeric, single-stranded DNA binding complex required for eukaryotic DNA replication, repair, and recombination. RPA is composed of three subunits, RPA1, RPA2, and RPA3.
Aklilu, Behailu B., Culligan, Kevin M.
core   +4 more sources