Single‐molecule DNA flow‐stretch assays for high‐throughput DNA–protein interaction studies
We describe an optimised single‐molecule DNA flow‐stretch assay that visualises DNA–protein interactions in real time. Linear DNA fragments are tethered to a surface and stretched by buffer flow for fluorescence imaging. Using λ and φX174 DNA, this protocol enhances reproducibility and accessibility, providing a versatile approach for studying diverse ...
Ayush Kumar Ganguli +8 more
wiley +1 more source
Project quality, regulation quality
In the Italian context, the first law directly affecting the urban planning and building sector dates back to approximately 160 years ago, precisely Law 2248/1865.
Elena Mussinelli
doaj +1 more source
European works councils: progress and a long road ahead [PDF]
Stan De Spiegelaere and Romek Jagodzinski of the European Trade Union Institute give a progress report on European Works Councils. Although EWCs continue to grow, they suffer from a lack of resources and more of third of them lack training rights in ...
De Spiegelaere, Stan, Jagodzinski, Romek
core
Translating European Labor Relations Practices to the United States Through Global Framework Agreements? German and Swedish Multinationals Compared [PDF]
Extensive research has shown that European multinational enterprises (MNEs) have a propensity to avoid collective employee representation when going abroad.
Helfen, Markus O. +2 more
core +1 more source
Evolutionarily divergent DUF4465 domains have a common vitamin B12‐binding function
We show that DUF4465 family proteins, widespread across bacteria from gut microbiomes, hydrothermal vents, and soil, share a common vitamin B12‐binding function. These augmented β‐jellyroll proteins bind vitamin B12 via extended loops. Our findings establish sequence‐diverse DUF4465 proteins as a widespread class of B12‐binding proteins, highlighting ...
Charlea Clarke +4 more
wiley +1 more source
The East in Open Conflict: The Great Strike of 1993 [PDF]
[Excerpt] Because it is impossible in one book to examine all German institutions of negotiation, this book focuses on one important set of relations at the heart of social market regulation: the social partnership between labor and management. Social
Turner, Lowell
core +1 more source
THE EUROPEAN WORKS COUNCIL IN THE NETHERLANDS
This work contains an English translation of the current text of the Dutch implementing statute. It also contains an explanation of all the important issues addressed in the statute and an analysis of the provisions at variance with the Directive.
Marianne Ruizeveld, Leonard Verburg
openaire +1 more source
Remote Assessment of Ataxia Severity in SCA3 Across Multiple Centers and Time Points
ABSTRACT Objective Spinocerebellar ataxia type 3 (SCA3) is a genetically defined ataxia. The Scale for Assessment and Rating of Ataxia (SARA) is a clinician‐reported outcome that measures ataxia severity at a single time point. In its standard application, SARA fails to capture short‐term fluctuations, limiting its sensitivity in trials.
Marcus Grobe‐Einsler +20 more
wiley +1 more source
Cracking the Code: Genotype–Phenotype Correlation Models in Sarcoglycanopathies
ABSTRACT Objective Sarcoglycanopathies are among the most severe limb‐girdle muscular dystrophies (LGMD), though milder presentations have been described. These diseases are primarily caused by missense variants, but the limited predictability of their effect on protein maturation, complex formation, and transport has hindered reliable genotype ...
Leonela Luce +72 more
wiley +1 more source
Added Prognostic Value of EEG Reactivity in Comatose Patients Following Cardiac Arrest
ABSTRACT Objectives To evaluate the added prognostic value of EEG reactivity for favorable outcome compared with background analysis during and after targeted temperature management (TTM). Methods Prospective observational cohort study of comatose post–cardiac arrest patients admitted to a single academic center between 2017 and 2022, all undergoing ...
Sarah Caroyer +11 more
wiley +1 more source

