Brugia malayi: The Diagnostic Potential of Recombinant Excretory/Secretory Antigens
Experimental Parasitology, 1994The diagnostic potential of recombinant E/S antigens of the lymphatic filaria Brugia malayi was investigated by Western blot. A cDNA expression library was constructed using B. malayi male adult worm mRNA, and E/S recombinants were identified with a rabbit antiserum raised against E/S products collected in vitro from B.
S, Kumari +5 more
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Immunomodulatory effects of Trichinella spiralis excretory-secretory antigens on macrophages
Experimental Parasitology, 2019Helminths and their products can suppress the host immune response and other immunopathologies which may benefit parasite survival. Parasitic nematode Trichinella spiralis (T. spiralis) exert immunomodulatory effect on the host immune response through excretory-secretory (ES) products.
Caixia, Han +8 more
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DETECTION OF TOXOPLASMIC IMMUNITY BY MULTIPUNCTURE SKIN TEST WITH EXCRETORY-SECRETORY ANTIGEN
Lancet, The, 1985In 414 volunteers the sensitivity and specificity of a multipuncture skin test with excretory-secretory (ES) toxoplasma antigen was compared with serological detection by an indirect fluorescent antibody (IFA) test. The ES antigen was extracted from supernatants of cell cultures infected with Toxoplasma gondii, purified, and standardised, and the ...
P Ambroise-Thomas
exaly +3 more sources
Idiotypic replica of a Toxocara canis excretory/secretory antigen epitope
International Journal for Parasitology, 1995This study describes the production, characterization and use of an anti-idiotype serum raised against the monoclonal antibody TC-1 which recognizes a T. canis excretory/secretory antigen (ES Ag) epitope. Anti-idiotypic (anti-Id or Ab2) antibodies were produced in rabbits using TC-1 F(ab')2 fragments; these anti-Id inhibited ES Ag binding to ...
R, Bardon, J L, Guillen, C, Aguila
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Radiolabelling of the excretory-secretory and somatic antigens ofAnisakis simplexlarvae
Journal of Helminthology, 1992ABSTRACTAnisakis simplexlarvae were culturedin vitroin medium containing35-methionine for ten days. The medium and the larval tissues were analysed for biosynthetically labelled polypeptide by sodium dodecyl sulphate polyacrylamide gel electrophoresis and autoradiography.
K, Sugane, S H, Sun, T, Matsuura
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Toxoplasma gondii excretory secretory antigenic proteins of diagnostic potential
APMIS, 2011Saadatnia G, Mohamed Z, Ghaffarifar F, Osman E, Kazemi Moghadam Z, Noordin R. Toxoplasma gondii excretory secretory antigenic proteins of diagnostic potential. APMIS 2012; 120: 47–55.Infection with Toxoplasma gondii is widespread and important in humans, especially pregnant women and immunosuppressed patients.
Geita, Saadatnia +5 more
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Monoclonal Antibodies Against Excretory/Secretory Antigens of Angiostrongylus cantonensis
Hybridoma, 2010In the present study, four murine monoclonal antibodies (MAbs) were generated against the excretory/secretory (ES) products of Angiostrongylus cantonensis adult worms; two represented IgG1 and two represented IgM MAbs, and they were designated 12D5, 15F8, 21B7 and 14G10, respectively.
Mu-Xin, Chen +10 more
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Excretory/secretory antigens of adult Paragonimus ohirai recognized by a monoclonal antibody
International Journal for Parasitology, 1995An IgM monoclonal antibody (mAb) that reacted with the gut epithelium and luminal contents of adult Paragonimus ohirai was prepared from a mouse immunized with the excretory/secretory (ES) products of the parasite. The mAb reacted with the same sites not only of immature worms and newly excysted juveniles of P.
H, Ichikawa, T, Ikeda
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Detection of Taenia solium Cysticercosis in Pigs by ELISA with an Excretory-Secretory Antigen
Veterinary Research Communications, 1999Serodiagnosis of Taenia solium cysticercosis in pigs was conducted by an enzyme-linked immunosorbent assay with an excretory-secretory antigen. The antigen was obtained by in vitro cultivation of the cysticerci in a synthetic medium RPMI 1640. The sensitivity and specificity of the ELISA in detecting infection in pigs reared on free range was 92% and ...
D'Souza, P E, Hafeez, M
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Eosinophil differentiation in response to Fasciola hepatica and its excretory/secretory antigens
International Journal for Parasitology, 1993Bone marrow cells from mice infected with Fasciola hepatica, from mice injected with F. hepatica excretory/secretory (ES) antigens, and from uninfected or uninjected control animals were cultured in the presence of F. hepatica ES antigens or the eosinophil differentiation cytokine IL-5.
E A, Milbourne, M J, Howell
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