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Excretory-secretory and somatic antigens in the diagnosis of human filariasis.
Clinical and experimental immunology, 1984In order to compare the immunodiagnostic value of excretory-secretory (E-S) antigens derived from adult Brugia malayi worms with somatic antigens derived from adults, microfilariae (Mf) and infective larvae (L3) of these parasites, well defined serum pools from patients with filarial (brugia, bancrofti, loa and perstans) and non-filarial (ascaris ...
N A, Kaushal, R, Hussain, E A, Ottesen
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Analysis of excretory-secretory and somatic antigens of Gastrothylax crumenifer.
Journal of helminthology, 2000The excretory/secretory (ES) metabolic products released by Gastrothylax crumenifer (Trematoda: Digenea) during in vitro incubations and the somatic extract of the adult parasite were analysed using polyacrylamide gel electro-phoresis (PAGE). Immunogenicity of ES and somatic extracts were evaluated by immunoblotting and ELISA using sera raised against ...
M K, Saifullah, G, Ahmad, W A, Nizami
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Antigenic characterization of excretory-secretory products of Setaria cervi.
Tropical medicine and parasitology : official organ of Deutsche Tropenmedizinische Gesellschaft and of Deutsche Gesellschaft fur Technische Zusammenarbeit (GTZ), 1987Excretory-secretory products (ES) of Setaria cervi, a bovine filarial parasite, were prepared by in vitro maintenance of the worms in a protein free defined medium at 37 degrees C. RPMI-1640 medium proved to be the best as the worms remained motile in it for the maximum period of time without change of medium, and approximately 12-15 mg protein per 100
A, Malhotra +3 more
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Presentation of a membrane cysticercus antigen and its homology with excretory--secretory antigen.
Acta Leidensia, 1991A cysticercus antigen was purified from the membrane. It is a glycoprotein containing hexoses and sialic acids with a molecular weight of 70 Kd and isoelectric focussing point 4.8. In western blot studies, the purified antigen bound with antibodies present in cerebrospinal fluid of all neurocysticercosis patients tested.
N A, Khan, J, Sotelo
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Analysis of excretory-secretory and somatic antigens of Gastrothylax crumenifer.
Journal of helminthologyThe excretory/secretory (ES) metabolic products released by Gastrothylax crumenifer (Trematoda: Digenea) during in vitro incubations and the somatic extract of the adult parasite were analysed using polyacrylamide gel electrophoresis (PAGE). Immunogenicity of ES and somatic extracts were evaluated by immunoblotting and ELISA using sera raised against ...
, Saifullah, , Ahmad, , Nizami
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Xi bao yu fen zi mian yi xue za zhi = Chinese journal of cellular and molecular immunology, 2016
To isolate and identify serological antigens in the excretory-secretory antigens of Trichinella spiralis muscle larvae by the combination of co-immunoprecipitation and mass spectrometric technology.The serum IgG of New Zealand rabbits infected with Trichinella spiralis was isolated by ammonium sulfate precipitation.
Xuegui, Huang +4 more
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To isolate and identify serological antigens in the excretory-secretory antigens of Trichinella spiralis muscle larvae by the combination of co-immunoprecipitation and mass spectrometric technology.The serum IgG of New Zealand rabbits infected with Trichinella spiralis was isolated by ammonium sulfate precipitation.
Xuegui, Huang +4 more
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