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Studying Exoribonuclease Activity Using Fluorescence Anisotropy Assay
Fluorescence anisotropy is a powerful technique, widely used for investigating ligand-macromolecule binding and high-throughput screens for drugs. Here, we employ fluorescence anisotropy to quantitatively study the activity of exoribonucleases exemplified by the Xrn2 enzyme.Krzysztof, Kuś, Lidia, Vasiljeva
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Cooperation of Endo- and Exoribonucleases in Chloroplast mRNA Turnover
2004Chloroplasts were acquired by eukaryotic cells through endosymbiosis and have retained their own gene expression machinery. One hallmark of chloroplast gene regulation is the predominance of posttranscriptional control, which is exerted both at the gene-specific and global levels.
Thomas J, Bollenbach +2 more
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Function and Characterization of Poly(A)-Specific 3´ Exoribonucleases
1997Poly(A) tails are commonly found at the 3´ end of various classes of RNA (reviewed in Brawerman 1981; Manley 1995b). They are evolutionarily widespread and appear on RNAs of several different organisms. In mammalian cells almost all mRNAs end with an approximately 200-adenosine-residue-long poly(A) tail.
A, Virtanen, J, Aström
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Lactobacillus plantarum exoribonuclease. Further purification and characterization
Biochemistry, 1971E C, Gardonio, D M, Logan
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