Results 321 to 330 of about 6,729,673 (335)

Exosome-delivered CD44v6/C1QBP complex drives pancreatic cancer liver metastasis by promoting fibrotic liver microenvironment

Gut, 2021
Objective Pancreatic ductal adenocarcinoma (PDAC) shows a remarkable predilection for liver metastasis. Pro-oncogenic secretome delivery and trafficking via exosomes are crucial for pre-metastatic microenvironment formation and metastasis.
Zhibo Xie, Ya Gao, Chiakang Ho, Lequn Li, C. Jin, Xiaoyi Wang, Caifeng Zou, Yishen Mao, Xiaobo Wang, Qingfeng Li, D. Fu, Yi-Fan Zhang   +11 more
semanticscholar   +1 more source

Proteomic Analysis of Yeast Mutant RNA Exosome Complexes

Journal of Proteome Research, 2013
The yeast exosome is a conserved multiprotein complex essential for RNA processing and degradation. The complex is formed by a nine-subunit core that associates with two hydrolytic 3'-5' exoribonucleases. Although catalytically inert, the assembly of this nine-subunit core seems to be essential for the exosome activity, as mutations in regions that do ...
Rogério F, Lourenço, Adriana F P, Leme, Carla C, Oliveira   +2 more
openaire   +2 more sources

Heterogeneous complexes of the RNA exosome in Sulfolobus solfataricus

Biochimie, 2012
The archaeal exosome is a protein complex involved in the degradation and the posttranscriptional tailing of RNA. The proteins Rrp41, Rrp42, Rrp4, Csl4 and DnaG are major subunits of the exosome in Sulfolobus solfataricus. In vitro, Rrp41 and Rrp42 form a catalytically active hexamer, to which an RNA-binding cap of Rrp4 and/or Csl4 is attached.
Chamindri, Witharana, Verena, Roppelt, Günther, Lochnit, Gabriele, Klug, Elena, Evguenieva-Hackenberg   +4 more
openaire   +2 more sources

An engineered exosome for delivering sgRNA:Cas9 ribonucleoprotein complex and genome editing in recipient cells.

Biomaterials Science, 2020
CRISPR-Cas9 is a versatile genome-editing technology that is a promising gene therapy tactic. However, the delivery of CRISPR-Cas9 is still a major obstacle to its broader clinical application.
Yangyang Ye, Xiang Zhang, Fei Xie, Bin Xu, Ping Xie, Ting Yang, Qian Shi, Chen-Yu Zhang, Yujing Zhang, Jiangning Chen, Xiaohong Jiang, Jing Li   +11 more
semanticscholar   +1 more source

Comparison of the yeast and human nuclear exosome complexes

Biochemical Society Transactions, 2012
Most RNAs in eukaryotic cells are produced as precursors that undergo processing at the 3′ and/or 5′ end to generate the mature transcript. In addition, many transcripts are degraded not only as part of normal recycling, but also when recognized as aberrant by the RNA surveillance machinery.
Katherine E, Sloan, Claudia, Schneider, Nicholas J, Watkins   +2 more
openaire   +2 more sources

Exosome complex and pervasive transcription in eukaryotic genomes

Current Opinion in Cell Biology, 2009
Exosome complex is widely conserved, functionally versatile, and essential constituent of the machinery regulating gene expression in the nucleus as well as in the cytoplasm. While the most fundamental enzymatic property of exosome is ribonucleolytic activity, its in vivo functions are varied, highly specific, and tightly regulated, and include RNA ...
openaire   +2 more sources

Fully Integrated Centrifugal Microfluidics for Rapid Exosome Isolation, Glycan Analysis, and Point-of-Care Diagnosis.

ACS Nano
Exosomes present in the circulatory system demonstrate considerable promise for the diagnosis and treatment of diseases. Nevertheless, the complex nature of blood samples and the prevalence of highly abundant proteins pose a significant obstacle to ...
Xudong Zhao, Xiang Liu, Tucan Chen, Han Xie, Shunji Li, Ying Zhang, Hongwei Zhang, Yulin Cao, Wei Du, Xiaojun Feng, Xin Liu, Yiwei Li, Peng Chen, Qiubai Li, Bi-Feng Liu   +14 more
semanticscholar   +1 more source

Purification of Endogenous Tagged TRAMP4/5 and Exosome Complexes from Yeast and In Vitro Polyadenylation-Exosome Activation Assays

2019
The RNA exosome processes a wide variety of RNA and mediates RNA maturation, quality control and decay. In marked contrast to its high processivity in vivo, the purified exosome exhibits only weak activity on RNA substrates in vitro. Its activity is regulated by several auxiliary proteins, and protein complexes.
Dagmar, Zigáčková, Veronika, Rájecká, Štěpánka, Vaňáčová   +2 more
openaire   +2 more sources

Exosomes and tumor virus interlink: A complex side of cancer

Pathology - Research and Practice
Extracellular Vesicles (EVs) based cancer research reveals several complicated sides of cancer. EVs are classified as several subpopulations such as microvesicles, apoptotic bodies, and exosomes. In cancer, exosomes play a significant role as a cellular messenger in tumor development and progression. Tumor-derived exosomes (TEXs) are also a theranostic
Ibrahim S, Topiwala, Aparna, Ramachandran, Meghana Shakthi, A, Ranjini, Sengupta, Rajib, Dhar, Arikketh, Devi   +5 more
openaire   +2 more sources

Hydrogel–exosome complexes: a novel strategy for cardiovascular regeneration

Nanoscale
Cardiovascular disease (CVD) remains one of the leading causes of high mortality and morbidity worldwide, posing a substantial threat to global health.
Yonglin Fu, Zixiong Qiu, Yifang Cao, Mei Jiang, Xiaojun Cui   +4 more
openaire   +1 more source