Results 61 to 70 of about 114,889,184 (267)

Golgi enzymes are retrieved from the plasma membrane to the trans‐Golgi network

FEBS Letters, EarlyView.
Golgi enzymes are traditionally considered resident proteins retained within the Golgi apparatus. Here, we demonstrate that a subset transiently reaches the cell surface and is subsequently retrieved to the trans‐Golgi network via retrograde transport. Using a nanobody‐based toolkit, we uncover a dynamic trafficking cycle of several Golgi enzymes.
Dominik P. Buser, Tina Junne
wiley   +1 more source

Predicting the in vivo pulmonary toxicity induced by acute exposure to poorly soluble nanomaterials by using advanced in vitro methods

Particle and Fibre Toxicology, 2018
Background Animal models remain at that time a reference tool to predict potential pulmonary adverse effects of nanomaterials in humans. However, in a context of reduction of the number of animals used in experimentation, there is a need for reliable ...
Thomas Loret, Françoise Rogerieux, Bénédicte Trouiller, Anne Braun, Christophe Egles, Ghislaine Lacroix   +5 more
doaj   +1 more source

Functional comparison of EncB and EncC cargo proteins in iron storage within the Myxococcus xanthus encapsulin

FEBS Letters, EarlyView.
Encapsulins are protein nanocompartments that play an important role in iron storage. In the Myxococcus xanthus encapsulin system, two cargo proteins called EncB and EncC contribute to iron mineralization. Here, we show that EncB and EncC generate iron‐containing minerals with distinct chemical compositions, suggesting that the composition of stored ...
Harry B. McDowell, Thomas Walther, Egbert Hoiczyk   +2 more
wiley   +1 more source

Methods for culturing retinal pigment epithelial cells: a review of current protocols and future recommendations

Journal of Tissue Engineering, 2016
The retinal pigment epithelium is an important part of the vertebrate eye, particularly in studying the causes and possible treatment of age-related macular degeneration.
Aaron H Fronk, Elizabeth Vargis
doaj   +1 more source

Drosophila melanogaster: a simple system for understanding complexity

Disease Models & Mechanisms, 2019
Understanding human gene function is fundamental to understanding and treating diseases. Research using the model organism Drosophila melanogaster benefits from a wealth of molecular genetic resources and information useful for efficient in vivo ...
Stephanie E. Mohr, Norbert Perrimon
doaj   +1 more source

Ligandi dei siti recettoriali orto- e allosterici: caratterizzazione in vitro e in vivo di nuovi derivati dualsterici bisammonioalcanici

, 2015
In this study we characterized in vitro and in vivo three different groups of dualsteric compounds, which were synthesized as M2 muscarinic agonists. These bitopic ligands, which incorporate in a unique molecule an orthosteric muscarinic agonist and a ...
VIVO, Valentina
core  

vivo-project/VIVO: Release vivo-1.14.2

<p><strong>Release vivo 1.14.2</strong><br/>It is recommended that you review the 1.14 Documentation and the VIVO/Vitro READMEs on github before you start.<br/>If you are new to VIVO/Vitro it is suggested that you take ...
Jim Blake, Stefan-Wolff, Brian Caruso, Andrew Woods, Nate Prewitt, William Welling, Don Elsborg, hudajkhan, Ralph O'Flinn, Christian Hauschke, Georgy Litvinov, Alex Viggio, tworrall, Ben, Ted Lawless, Miloš Popović, Arfon Smith, vivo-uab, Jon Corson-Rikert, Steve McKay, Mike Conlon, Dragan Ivanovic, Brian Lowe, Stephen V. Williams, Holly Mistlebauer, Michel Héon, Laura Wrubel, Graham Triggs   +27 more
core   +1 more source

Structural and biochemical analysis of a B12 superbinder

FEBS Letters, EarlyView.
BtuG proteins are vitamin B12 scavengers in Bacteroides thetaiotaomicron, a dominant human gut bacterium. We present crystal structures of three BtuG homologs bound to cobalamin and its precursor cobinamide, revealing picomolar binding affinities, among the highest known for any natural protein.
Jose M. Martinez Felices, Jacob J. Whittaker, Dirk J. Slotboom, Albert Guskov   +3 more
wiley   +1 more source

L‐aspartate oxidase provides new insights into fumarate reduction in anaerobic darkness in Synechocystis sp. PCC6803

FEBS Letters, EarlyView.
Synechocystis strains deficient in succinate dehydrogenase (SDH) secrete more succinate than the WT under dark anaerobic conditions, supporting that SDH then primarily acts as SDH, not as a fumarate reductase. L‐aspartate oxidase (Laspo) from Synechocystis is functional under anaerobic conditions, reducing fumarate to succinate.
Kateryna Kukil, Jeffrey A. Hawkes, Cecilia Blikstad, Pia Lindberg   +3 more
wiley   +1 more source

The heterodimeric amino acid transporters (HAT) of the SLC7/SLC3 family: A structure−function relationships and relevance to human pathology

FEBS Letters, EarlyView.
Heterodimeric amino acid transporters consist of SLC7 and SLC3 family proteins arranged in a conserved structural organization. They regulate nutrient transport across cell membranes, supporting essential cellular functions. These transporters also contribute to xenobiotic/drug uptake and distribution.
Mariafrancesca Scalise, Tiziano Mazza, Raffaella Scanga, Lara Console, Michele Galluccio, Cesare Indiveri   +5 more
wiley   +1 more source