The impact of leukocyte‐associated RNA retention on platelet RNA‐based cancer detection
Molecular Oncology, EarlyView.Platelets carry RNA signals that may help detect cancer, but platelet samples can also retain RNA from white blood cells. We found that this retained white blood cell signal did not significantly affect machine learning cancer classification, suggesting that the cancer‐related RNA signal in platelets is largely independent of leukocyte‐associated RNA ...
Michał Sieczczyński +12 more
wiley +1 more source
Lights, Camera, Distraction: Rethinking the Interpretability of Effect Size for Comedy Film Interventions. [PDF]
Nurs Health SciWang E, Zhang L, Gao D.
europepmc +1 more source
Single‐molecule DNA flow‐stretch assays for high‐throughput DNA–protein interaction studies
FEBS Open Bio, EarlyView.We describe an optimised single‐molecule DNA flow‐stretch assay that visualises DNA–protein interactions in real time. Linear DNA fragments are tethered to a surface and stretched by buffer flow for fluorescence imaging. Using λ and φX174 DNA, this protocol enhances reproducibility and accessibility, providing a versatile approach for studying diverse ...
Ayush Kumar Ganguli +8 more
wiley +1 more source
Autonomic imbalance as a predictor of pain expectations and pain experience in chronic back pain: study protocol of a pilot study on the application of heart rate variability biofeedback in a mixed-methods design (BACK.BEAT). [PDF]
BMJ OpenSchmitz S, Löwe B, Hüsing P.
europepmc +1 more source
RoundMi: A quantitative method to analyze mitochondrial morphology in mitotic cells
FEBS Open Bio, EarlyView.RoundMi is a workflow for rapid analysis of mitochondrial morphology in mitotic cells. By combining adaptive preprocessing with automated segmentation and quantification, it enables accurate measurements from single focal plane images, reducing acquisition time and computational demands while remaining compatible with high‐throughput fixed and live ...
Elmira Parvindokht Bararpour +2 more
wiley +1 more source
Perceived reward adequacy as a key mechanism for retaining medical doctors in health systems: A structural equation modelling study. [PDF]
Afr J Prim Health Care Fam MedFenyi S, Keelson S.
europepmc +1 more source
Versatile vector tools for efficient protein screening across multiple expression systems
FEBS Open Bio, EarlyView.A unified vector toolkit enables rapid protein expression screening across E. coli, insect, and mammalian cells. A single primer pair amplifies the target gene, which is inserted into any vector via a standardized interface. This streamlined workflow eliminates repeated cloning steps, accelerating the identification of optimal expression conditions for
Zhimin Zhu +5 more
wiley +1 more source
Pain Expectation in Individuals With High Autistic Traits: Decision-Evaluation Processes and Oxytocin Modulation. [PDF]
Ann N Y Acad SciZhu J +6 more
europepmc +1 more source