Results 131 to 140 of about 31,723,480 (301)
Drosophila melanogaster c306 GAL4 is not specific to ovarian cells
microPublication BiologyA widely used genetic tool in Drosophila melanogaster is the GAL4/UAS system. This bipartite system allows for tissue and cell-specific expression and is used for both overexpression and RNA interference (RNAi) knockdown studies. Here we provide evidence
Ashley C. Goll +2 more
doaj +1 more source
Structural and biochemical analysis of a B12 superbinder
FEBS Letters, EarlyView.BtuG proteins are vitamin B12 scavengers in Bacteroides thetaiotaomicron, a dominant human gut bacterium. We present crystal structures of three BtuG homologs bound to cobalamin and its precursor cobinamide, revealing picomolar binding affinities, among the highest known for any natural protein.
Jose M. Martinez Felices +3 more
wiley +1 more source
microPublication BiologyLoss of function in the Drosophila melanogaster gene julius seizure (jus) causes the fly to have a seizure-like condition known as bang-sensitivity. Here, we characterize the expression patterns of two different jus reporters, a jus-GAL4 construct and a ...
Derek M. Dean +12 more
doaj +1 more source
, 2020 Supplementary data for the article "CTCF mediated genome architecture regulates the dosage of mitotically stable mono-allelic expression of autosomal genes" by Chandradoss et ...
Sandhu, K (via Mendeley Data) +1 more
core +1 more source
FEBS Letters, EarlyView.Mycobacterial 3‐methylcrotonyl‐CoA carboxylase uses a mobile biotin‐carrying domain to shuttle a carboxyl group between two catalytic sites, enabling carboxylation of 3‐methylcrotonyl‐CoA during leucine breakdown. Cryo‐electron microscopy captures the carrier at both sites and reveals an inward loop movement that may prevent futile rebinding to the ...
Ajit Yadav +2 more
wiley +1 more source
Exploring non-autonomous protein homeostasis driven by glutamatergic neurons
microPublication BiologyNeuronal overexpression of xbp-1s, a regulator of the endoplasmic reticulum unfolded protein response (UPRER), induces non-autonomous UPRER activation in distal tissues of Caenorhabditis elegans.
Adam J Hruby +8 more
doaj +1 more source
The Shewanella oneidensis Fic enzyme SoFic targets the switch‐I region of EF‐Tu for AMPylation
FEBS Letters, EarlyView.Fic enzymes mediate diverse post‐translational modifications across all domains of life, including AMPylation. Prokaryotic EF‐Tu can be AMPylated and deAMPylated by the conserved Fic enzyme SoFic. Structural and biochemical approaches were used to characterize the effect of AMPylation on EF‐Tu, SoFic's enzymatic activities, and the enzyme‐target ...
Svenja Runge +6 more
wiley +1 more source
KDM5A overexpression modulates key myogenic differentiation factors and sarcomere gene expression
microPublication BiologyMyogenic differentiation is a complex and tightly regulated process involving multiple transcriptional regulators. KDM5A (lysine demethylase 5A) is a histone demethylase that regulates gene transcription and has been implicated in skeletal muscle ...
Geert de Vries +5 more
doaj +1 more source
Chiral separation of chloroalkanes with the chromatographic column onboard Martian rovers
FEBS Letters, EarlyView.Computer image of the Rosalind Franklin Rover of ESA's ExoMars mission. ExoMars is scheduled to land on planet Mars in Oxia Planum in 2029. This area represents an interesting spot to look for biosignatures. Investigations of ExoMars include measurement on molecular chirality. We show that chiral chloroalkanes, that have been identified on Mars, can be
Asma Merzougui +4 more
wiley +1 more source
gCube 4.2.1 - tabular-data-expression-widget v. 1.8.0
, 2017 <p> The gCube System - tabular-data-expression-widget<br> --------------------------------------------------<br> <br> tabular-data-expression-widget allows to create expression on tabular resource<br> <br> <br>
Giancarlo Panichi (5200930) +1 more
core +1 more source