The experiment was conducted to purify high activity extracellular enzymes, which were produced by a strain that we previously screened was able to degrade aflatoxin effectively, and speculate the functional groups of the enzyme associated with ...
Xue‐Jiao Zhang +4 more
doaj +1 more source
Luciferase activity under direct ligand-dependent control of a muscarinic acetylcholine receptor
Background Controlling enzyme activity by ligand binding to a regulatory domain of choice may have many applications e.g. as biosensors and as tools in regulating cellular functions.
Strotmann Rainer +3 more
doaj +1 more source
Detection of Extracellular enzymes Activities in VariousFusariumspp. [PDF]
Thirty seven species of Fusarium were evaluated for their ability of producing extracellular enzymes using chromogenic medium containing substrates such as starch, cellobiose, CM-cellulose, xylan, and pectin. Among the tested species Fusarium mesoamericanum, F. graminearum, F. asiaticum, and F. acuminatum showed high β-glucosidase acitivity.
Kwon, Hyuk Woo +5 more
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Enzymatic Analysis of Yeast Cell Wall-Resident GAPDH and Its Secretion
In yeast, many proteins are found in both the cytoplasmic and extracellular compartments, and consequently it can be difficult to distinguish nonconventional secretion from cellular leakage.
Michael J. Cohen +2 more
doaj +1 more source
EVIDENCE FOR EXTRACELLULAR ENZYMIC ACTIVITY OF THE ISOLATED PERFUSED RAT HEART [PDF]
1. The dissimilation of a number of externally added hexose phosphates and 5'-nucleotides by the perfused rat heart is described, and non-specific esterase and 5'-nucleotidase activity associated with the superficial cell membrane or vascular system has been demonstrated. 2. The rate of production of (14)CO(2) from [U-(14)C]glucose 6-phosphate suggests
J R, WILLIAMSON, D L, DIPIETRO
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Heterotrophic microorganisms in marine sediments produce extracellular enzymes to hydrolyze organic macromolecules, so their products can be transported inside the cell and used for energy and growth. Therefore, extracellular enzymes may mediate the fate
Jenna M. Schmidt +4 more
doaj +1 more source
Solvent and thermal stability, and pH kinetics, of proline-specific dipeptidyl peptidase IV-like enzyme from bovine serum [PDF]
Proline-specific dipeptidyl peptidase-like (DPP IV; EC 3.4.14.5) activity in bovine serum has attracted little attention despite its ready availability and the paucity of useful proline-cleaving enzymes.
Ruth, Deborah M. +4 more
core +1 more source
Detection of Extracellular Enzyme Activity inPenicilliumusing Chromogenic Media [PDF]
A total of 106 Penicillium species were tested to examine their ability of degrading cellobiose, pectin and xylan. The activity of β-glucosidase was generally strong in all the Penicillium species tested. P. citrinum, P. charlesii, P. manginii and P. aurantiacum showed the higher ability of producing β-glucosidase than other tested species.
Yoon, Ji Hwan +3 more
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Protein disulfide isomerase activity is essential for viability and extracellular matrix formation in the nematode Caenorhabditis elegans. [PDF]
Protein disulfide isomerase (PDI) is a multifunctional protein required for many aspects of protein folding and transit through the endoplasmic reticulum.
Page, Antony P. +5 more
core +1 more source
INTERACTIONS OF BACTERIA AND FUNGI ON DECOMPOSING LITTER: DIFFERENTIAL EXTRACELLULAR ENZYME ACTIVITIES [PDF]
Fungi and bacteria are key agents in plant litter decomposition in freshwater ecosystems. However, the specific roles of these two groups and their interactions during the decomposition process are unclear. We compared the growth and patterns of degradative enzymes expressed by communities of bacteria and fungi grown separately and in coexistence on ...
Romaní i Cornet, Anna M. +3 more
openaire +3 more sources

