Results 151 to 160 of about 11,735,145 (343)
Single‐molecule DNA flow‐stretch assays for high‐throughput DNA–protein interaction studies
FEBS Open Bio, EarlyView.We describe an optimised single‐molecule DNA flow‐stretch assay that visualises DNA–protein interactions in real time. Linear DNA fragments are tethered to a surface and stretched by buffer flow for fluorescence imaging. Using λ and φX174 DNA, this protocol enhances reproducibility and accessibility, providing a versatile approach for studying diverse ...
Ayush Kumar Ganguli +8 more
wiley +1 more source
Constitutive parameter de-embedding using inhomogeneously-filled rectangular waveguides with longitudinal section modes [PDF]
Constitutive parameter extraction from S parameter data using a rectangular waveguide whose cross section is partially filled with a material sample as opposed to being completely filled was examined.
Dominek, A. K., Park, A.
core +1 more source
FEBS Open Bio, EarlyView.Amino acids sequence of two different proteins with the same sequence (chameleon sequence—black boxes) represent in 3D structure of the proteins different secondary structures: HHHH—helical and BBB—Beta‐structural. The chains folded in water environment adopt different III‐order structures in which the chameleon fragments appear to adopt similar status
Irena Roterman +4 more
wiley +1 more source
Scientific Journal of Gdynia Maritime University, 2017 The aim of this work was to determine a practical usefulness of literature Hansen solubility parameters to determine the composition of solvents for efficient extraction of active compounds present in chamomile inflorescences.
R. Zieliński
doaj
Optimising Speaker-Dependent Feature Extraction Parameters to Improve Automatic Speech Recognition Performance for People with Dysarthria. [PDF]
Sensors (Basel), 2021 Marini M, Vanello N, Fanucci L.
europepmc +1 more source
FEBS Open Bio, EarlyView.This protocol paper outlines methods to establish the success of a time‐resolved serial crystallographic experiment, by means of statistical analysis of timepoint data in reciprocal space and models in real space. We show how to amplify the signal from excited states to visualise structural changes in successful experiments.
Jake Hill +4 more
wiley +1 more source
Solid-Phase Extraction of Active Compounds from Natural Products by Molecularly Imprinted Polymers: Synthesis and Extraction Parameters. [PDF]
Polymers (Basel), 2021 Kamaruzaman S +5 more
europepmc +1 more source
Screening and epitope characterization of Nidogen‐2‐specific nanobodies
FEBS Open Bio, EarlyView.Camel immunization and phage display were employed to generate high‐affinity VHH nanobodies against Nidogen‐2. After library construction, biopanning, ELISA screening, sequencing, and recombinant expression, selected nanobodies were purified and characterized, leading to the preliminary exploration of a nanobody‐based sandwich ELISA for specific ...
Jianchuan Wen +9 more
wiley +1 more source
Nigerian Journal of Technological Development, 2016 Extraction process parameters can influence the quality of oil obtained from seeds during extraction process. The effect of extraction process parameters on the quality of parinari seed oil, a potential renewable industrial raw material, is yet to be ...
T. E. Odetoye +2 more
doaj +2 more sources
Digital twins to accelerate target identification and drug development for immune‐mediated disorders
FEBS Open Bio, EarlyView.Digital twins integrate patient‐derived molecular and clinical data into personalised computational models that simulate disease mechanisms. They enable rapid identification and validation of therapeutic targets, prediction of drug responses, and prioritisation of candidate interventions.
Anna Niarakis, Philippe Moingeon
wiley +1 more source