Results 41 to 50 of about 111,108 (251)
NanoLuc luciferase - A multifunctional tool for high throughput antibody screening
Frontiers in Pharmacology, 2016 Based on the recent development of NanoLuc Luciferase a small (19 kDa), highly stable, ATP independent, bioluminescent protein, an extremely robust and ultra high sensitivity screening system has been developed whereby primary hits of therapeutic ...
Nicolas eBoute +5 more
doaj +1 more source
Determining the affinities of high-affinity antibodies using KinExA and surface plasmon resonance
mAbs, 2023 Accurate and efficient affinity measurement techniques are essential for the biophysical characterization of therapeutic monoclonal antibodies, one of the fastest growing drug classes.
M. Frank Erasmus +8 more
doaj +1 more source
Synergistic perspectives—How single‐molecule biophysics complement biochemical understanding
FEBS Letters, EarlyView.In this review, we discuss how ensemble biochemistry and single‐molecule approaches are complementary, outline commonly used single‐molecule techniques, and illustrate their relevance through two representative case studies: chromatin organization by SMC complexes and pathway choice during DNA double‐strand break repair.
Sara De Bragança +2 more
wiley +1 more source
Proceedings of the 26th ACM SIGSPATIAL International Conference on Advances in Geographic Information Systems, 2018 Large analytic applications on road networks including simulations, logistics, location-based advertisement, and transportation planning require shortest distance/time methods that provide high throughput (i.e., distance/time computations per second). Our previous work discussed how to process graph distance computations in a PostgreSQL database on a ...
Shangfu Peng +2 more
openaire +1 more source
The Shewanella oneidensis Fic enzyme SoFic targets the switch‐I region of EF‐Tu for AMPylation
FEBS Letters, EarlyView.Fic enzymes mediate diverse post‐translational modifications across all domains of life, including AMPylation. Prokaryotic EF‐Tu can be AMPylated and deAMPylated by the conserved Fic enzyme SoFic. Structural and biochemical approaches were used to characterize the effect of AMPylation on EF‐Tu, SoFic's enzymatic activities, and the enzyme‐target ...
Svenja Runge +6 more
wiley +1 more source
Re-engineering adenovirus vector systems to enable high-throughput analyses of gene function
BioTechniques, 2008 With the enhanced capacity of bioinformatics to interrogate extensive banks of sequence data, more efficient technologies are needed to test gene function predictions.
Richard J. Stanton +4 more
doaj +1 more source
STAR Protocols, 2020 Summary: Single-cell RNA-sequencing technologies are ideally placed to resolve intratumoral heterogeneity. However, the lack of coverage across key mutation hotspots has precluded the correlation of genetic and transcriptional readouts from the same ...
Alba Rodriguez-Meira +3 more
doaj +1 more source
Congestion Analysis of Transport Layer in a Multicell 5G DL Communication System
Sensors, 2023 The fifth generation (5G) marks an important advance in mobile network capabilities. When it comes to high data rates, capacity, spectrum efficiency, and availability, 5G mobile broadband goes above and beyond what was previously possible with standard ...
Cristina Maria Andras +3 more
doaj +1 more source
RNA‐related functions of BRCA2 in transcription, replication, and genome stability
FEBS Letters, EarlyView.Beyond its role in homologous recombination, BRCA2 regulates R‐loop homeostasis, DNA–RNA hybrid resolution, RNA polymerase II dynamics, and transcriptional activation. Disruption of any of these functions can contribute to genome instability, inflammatory signaling, and tumorigenesis. BRCA2 is a central tumor suppressor protein best known for its roles
Lucia Alvaro‐Aranda +2 more
wiley +1 more source
Molecular Oncology, EarlyView.RNA profiling of circulating extracellular vesicles (EVs) from blood samples of men undergoing prostate biopsy identifies transcripts associated with clinically significant prostate cancer. Integrative analysis with public tumor datasets links EV‐derived gene signatures to tumor stage and progression‐free survival, highlighting CASP3, XRCC2, and RIT1 ...
Stefan Werner +14 more
wiley +1 more source