Results 61 to 70 of about 111,108 (251)

A Flexible Beamline for Ultrafast High-Throughput Microscopy with Extreme Ultraviolet Radiation

CLEO 2024
We report the design of a compact beamline for Time-Resolved Ptychography driven by a 13nm and 30nm High-Harmonic Generation source. The microscope is capable of quantitative, ultrafast, full-field microscopy supporting near-wavelength spatial and sub-50fs temporal resolutions.
Grova, Carmelo, Bevis, Charles S., Giani, Nicola, Adams, Daniel E., Svetina, Cristian, Mancini, Giulia F.   +5 more
openaire   +2 more sources

Fixed-target serial oscillation crystallography at room temperature

IUCrJ, 2019
A fixed-target approach to high-throughput room-temperature serial synchrotron crystallography with oscillation is described. Patterned silicon chips with microwells provide high crystal-loading density with an extremely high hit rate.
Jennifer L. Wierman, Olivier Paré-Labrosse, Antoine Sarracini, Jessica E. Besaw, Michael J. Cook, Saeed Oghbaey, Hazem Daoud, Pedram Mehrabi, Irina Kriksunov, Anling Kuo, David J. Schuller, Scott Smith, Oliver P. Ernst, Doletha M. E. Szebenyi, Sol M. Gruner, R. J. Dwayne Miller, Aaron D. Finke   +16 more
doaj   +1 more source

Single‐molecule DNA flow‐stretch assays for high‐throughput DNA–protein interaction studies

FEBS Open Bio, EarlyView.
We describe an optimised single‐molecule DNA flow‐stretch assay that visualises DNA–protein interactions in real time. Linear DNA fragments are tethered to a surface and stretched by buffer flow for fluorescence imaging. Using λ and φX174 DNA, this protocol enhances reproducibility and accessibility, providing a versatile approach for studying diverse ...
Ayush Kumar Ganguli, Mohammad Nour Alsamsam, Ugnė Bagdonaitė, Van Truc Vu, Chun‐Jen Huang, Polina Kuzhir, Mindaugas Zaremba, Aurimas Kopūstas, Marijonas Tutkus   +8 more
wiley   +1 more source

IAVCP (Influenza A Virus Consensus and Phylogeny): Automatic Identification of the Genomic Sequence of the Influenza A Virus from High-Throughput Sequencing Data

Viruses
Recently, high-throughput sequencing of influenza A viruses has become a routine test. It should be noted that the extremely high diversity of the influenza A virus complicates the task of determining the sequences of all eight genome segments.
Anastasiia Iu. Paremskaia, Pavel Yu. Volchkov, Andrei A. Deviatkin   +2 more
doaj   +1 more source

Loss of AMBRA1 activates MAPK and angiogenesis signaling pathways in melanoma cells

FEBS Open Bio, EarlyView.
Loss of AMBRA1 in melanoma cells activates multiple oncogenic pathways associated with tumor progression. Transcriptomic and protein network analyses revealed that AMBRA1 depletion enhances MAPK/ERK signaling, angiogenesis, TGF‐β/EMT signaling, and Wnt/axon guidance pathways.
Milad Ibrahim, Marco Corazzari, Iman Osman, Jane Armstrong, Noel Carter   +4 more
wiley   +1 more source

MagmaFlow: A desktop platform for artificial intelligence‐driven expression analysis

FEBS Open Bio, EarlyView.
MagmaFlow is a free, no‐code platform for gene expression analysis. It generates interactive volcano plots, links genes to literature, pathways, and diseases, prioritizes candidates using millions of publications, identifies affected biological processes, builds network diagrams, and exports publication‐ready figures and reports for macOS and Windows ...
Carlos E. Buss, Ao Li, Eduardo H. Gilglioni, Mayank Bansal, Sumeet Pal Singh, Latifa Bakiri, Alessandra K. Cardozo, Esteban N. Gurzov   +7 more
wiley   +1 more source

An Extremely Sensitive Ultra-High Throughput Growth Selection Assay for the Identification of Amidase Activity

Applied Microbiology and Biotechnology
Abstract In the last decades, biocatalysis has offered new perspectives for the synthesis of (chiral) amines, which are essential building blocks for pharmaceuticals, fine and bulk chemicals. In this regard, amidases have been employed due to their broad substrate scope and their independence from expensive cofactors. To expand
Yannick Branson, Bjarne Schnell, Celine Zurr, Thomas Bayer, Christoffel P.S. Badenhorst, Ren Wei, Uwe T. Bornscheuer   +6 more
openaire   +2 more sources

Accurate and noninvasive prostate cancer detection using plasma‐derived extracellular vesicle RNA

FEBS Open Bio, EarlyView.
Plasma extracellular vesicles were captured with WGA‐conjugated magnetic beads and profiled for RNA biomarkers. A three‐RNA panel (NM_024955, NR_047469, and NR_002564) distinguished prostate cancer from healthy controls and benign prostatic hyperplasia, supporting a simple, noninvasive approach to improve prostate cancer detection.
Hanping Wei, Haoran Wu, Wei Feng
wiley   +1 more source

Automated Wormscan [version 3; referees: 2 approved, 1 approved with reservations]

F1000Research, 2019
There has been a recent surge of interest in computer-aided rapid data acquisition to increase the potential throughput and reduce the labour costs of large scale Caenorhabditis elegans studies.
Timothy Puckering, Jake Thompson, Sushruth Sathyamurthy, Sinduja Sukumar, Tirosh Shapira, Paul Ebert   +5 more
doaj   +1 more source

Evolution‐guided yeast complementation reveals functional differences in human PSPH variants

FEBS Open Bio, EarlyView.
Ancient genomes can help guide which human genetic variants are tested experimentally. This study applies that idea to PSPH, a gene involved in serine biosynthesis, and uses high‐throughput yeast complementation to compare variant function. The findings reveal measurable differences among selected alleles and illustrate the value of evolution‐guided ...
Mauricio Campa‐Álvarez, Diana Ascencio, Miguel Vallebueno‐Estrada, Eduardo González‐Orozco, Christian Eduardo Martínez‐Guerrero, Rafael Montiel, Alexander DeLuna   +6 more
wiley   +1 more source