Association of candidate genes for fatty acid content in soybean by temperature-switch PCR (TSP) genotyping [PDF]
The development of molecular markers is essential for improvement of soybean cultivars with modified fatty acid content. The objective of this study was to identify and validate SNP markers in candidate genes for fatty acid content in soybean.
Rafael Delmond Bueno +6 more
doaj +5 more sources
Impact of Mutations in Soybean Oleate and Linoleate Desaturase Genes on Seed Germinability of Heat-Stressed Plants [PDF]
Soybean is the primary oilseed crop in the United States, with significant industrial value. Understanding the molecular mechanisms of heat stress tolerance in soybean plants is critical for developing stress-resistant cultivars.
Johnson O. Toyinbo +7 more
doaj +2 more sources
Associação de marcadores moleculares SNP com o conteúdo de ácido linolênico em sementes de soja Association of SNP markers with the linolenic acid content in soybean seeds [PDF]
O objetivo deste trabalho foi validar a associação de marcadores moleculares do tipo "single nucleotide polymorphism" (SNP) para os genes FAD3A, FAD3B e FAD3C com o conteúdo de ácido linolênico (18:3) em sementes de soja e analisar a influência dos ...
Marcos de Oliveira Pinto +3 more
doaj +1 more source
Key FAD2, FAD3, and SAD Genes Involved in the Fatty Acid Synthesis in Flax Identified Based on Genomic and Transcriptomic Data. [PDF]
FAD (fatty acid desaturase) and SAD (stearoyl-ACP desaturase) genes play key roles in the synthesis of fatty acids (FA) and determination of oil composition in flax (Linum usitatissimum L.).
Dvorianinova EM +13 more
europepmc +2 more sources
318-bp IR-derived siRNAs targeting FAD3B and FAD3C. [PDF]
(A) and (B) Small RNAs generated from the 318-bp IR matching the corresponding FAD3B and FAD3C target regions were plotted versus the average of their normalized abundance from three replications, respectively.
Ning Zhang (23771) +7 more
core +1 more source
5’RACE on GmFAD3A and GmFAD3C mRNAs in T5 RNAi lines. [PDF]
Arrows indicate the inferred cleavage sites and numbers above represent the fractions of cloned 5’ RACE PCR products terminating at this position. Degradation sites detected with high frequency are highlighted in red, and those present across the three ...
Ning Zhang (23771) +7 more
core +1 more source
FAD3 gene expression level of T5 homozygous RNAi lines. [PDF]
Data are averages of biological triplicates ± SD normalized to CONS7 mRNA. Independent-Samples T Test was used to test the significance. Asterisks indicate significant differences in relative expression between transgenic lines and control. (*, p < 0.05;
Ning Zhang (23771) +7 more
core +1 more source
Transcript profiling and gene characterization of three fatty acid desaturase genes in high, moderate, and low linolenic acid genotypes of flax (Linum usitatissimumL.) and their role in linolenic acid accumulation [PDF]
Three genes encoding fatty acid desaturase 3 (fad3a, fad3b, and a novel fad3c) were cloned from four flax genotypes varying in linolenic acid content. Real-time PCR was used to quantify expression levels of the three fad3 genes during seed development ...
Sylvie Cloutier +2 more
core +1 more source
Soybean Oil‐Quality Variants Identified by Large‐Scale Mutagenesis
To identify genetic variation for fatty acid composition in mature soybean seeds, 4566 M3 generation seed samples from a chemically mutagenized population were subjected to fatty acid profiling by gas chromatography. In the population, a wide range of variation in the content for each of the five major fatty acids was observed.
Karen Hudson, Mohamed Fawzy Ramadan
wiley +1 more source
Novel FAD3 Mutant Allele Combinations Produce Soybeans Containing 1% Linolenic Acid in the Seed Oil
Soybean [Glycine max (L.) Merr.] oil typically contains about 7% of linolenic acid, an oxidatively unstable fatty acid that is undesirable for many food applications. For cooking oil, reduction of the linolenic acid content by partial hydrogenation produces trans fatty acids, which are now known to increase the risk of coronary heart disease.
Kristin Bilyeu +2 more
wiley +1 more source

