Results 201 to 210 of about 24,500,580 (267)

An ecosystem for producing and sharing metadata within the web of FAIR Data. [PDF]

Gigascience
Jacob D, Ehrenmann F, David R, Tran J, Mirande-Ney C, Chaumeil P.   +5 more
europepmc   +1 more source

Delivery of DNA‐encoded vaccines and proteins in mice using cutaneous suction‐mediated transfection

FEBS Open Bio, EarlyView.
Cutaneous suction‐mediated transfection in mice for delivery of DNA‐encoded vaccines and proteins. A critical limitation of DNA vaccines and other therapeutics is transfection in vivo to produce the encoded antigens or therapeutic proteins. Cutaneous suction‐based methods have demonstrated effectiveness in many animal models and have been successfully ...
Emran O. Lallow, Isabel Brandtjen, Yaxin Mo, Melissa Gulley, Louis Osorio, Sagar Kudchodkar, Nandita C. Jhumur, Christine C. Roberts, Lisa K. Denzin, David I. Shreiber, Biju Parekkadan, Hao Lin, Joel N. Maslow   +12 more
wiley   +1 more source

A practical guide to FAIR data management in the age of multi-OMICS and AI. [PDF]

Front Immunol
Mugahid D, Lyon J, Demurjian C, Eolin N, Whittaker C, Godek M, Lauffenburger D, Fortune S, Levine S.   +8 more
europepmc   +1 more source

Evolution‐guided yeast complementation reveals functional differences in human PSPH variants

FEBS Open Bio, EarlyView.
Ancient genomes can help guide which human genetic variants are tested experimentally. This study applies that idea to PSPH, a gene involved in serine biosynthesis, and uses high‐throughput yeast complementation to compare variant function. The findings reveal measurable differences among selected alleles and illustrate the value of evolution‐guided ...
Mauricio Campa‐Álvarez, Diana Ascencio, Miguel Vallebueno‐Estrada, Eduardo González‐Orozco, Christian Eduardo Martínez‐Guerrero, Rafael Montiel, Alexander DeLuna   +6 more
wiley   +1 more source

The state of food composition databases: data attributes and FAIR data harmonization in the era of digital innovation. [PDF]

Front Nutr
Brinkley S, Gallo-Franco JJ, Vázquez-Manjarrez N, Chaura J, Quartey NKA, Toulabi SB, Odenkirk MT, Jermendi E, Laporte MA, Lutterodt HE, Annan RA, Barboza M, Amare E, Srichamnong W, Jaramillo-Botero A, Kennedy G, Bertoldo J, Prenni JE, Rajasekharan M, de la Parra J, Ahmed S.   +20 more
europepmc   +1 more source

Structural studies and functional engineering of NanX: an anhydro‐sialic acid transporter from Escherichia coli

FEBS Open Bio, EarlyView.
Biophysical characterisation shows that NanX, a membrane transport protein from the major facilitator superfamily (MFS), forms both monomers and dimers after purification. AlphaFold modelling and substrate docking provide information on residues likely involved in substrate recognition for NanX and another MFS member, NanT.
Michael C. Newton‐Vesty, Kelsi R. Hall, Zachary D. Tillett, Liam S. Turk, James S. Davies, Michael J. Currie, Hamish G. Brown, Sepideh Valimehr, Peter D. Mace, Andrew E. Whitten, Santosh Panjikar, Eric Hanssen, Rachel A. North, Renwick C.J. Dobson   +13 more
wiley   +1 more source

Enhancing mental health outcomes through a theory of change: supporting open-source data science platforms to promote FAIR data and evidence-based decisions. [PDF]

BMC Health Serv Res
Mugotitsa B, Andeso P, Owoko H, Momanyi R, Mailosi D, Ssebunnya J, Tsofa B, Greenfield J, Kiragga A, Todd J, INSPIRE network.   +10 more
europepmc   +1 more source

Purification and preparation of Marchantia polymorpha Auxin Response Factor 2 for phase separation studies

FEBS Open Bio, EarlyView.
We describe detailed protocols for the purification and preparation of Marchantia polymorpha Auxin Response Factor 2 (MpARF2). This protein is fused to an MBP solubility tag and an mNG fluorescent tag and is purified from Escherichia coli. The presented procedures make it possible to study MpARF2 assemblies, which could arise from phase separation ...
Bas Janssen, Robin Romein, Willy A. M. van den Berg, Simon Lindhoud, Jan Willem Borst, Carlo P. M. van Mierlo   +5 more
wiley   +1 more source

FAIR data management: a framework for fostering data literacy in biomedical sciences education. [PDF]

BMC Med Res Methodol
Gonzalez Soltero R, Pino García D, Bellido A, Ryan P, Rodríguez-Learte AI.   +4 more
europepmc   +1 more source

De novo synthesis of short‐chain aldehydes and hydrocarbons secreted by the brown marmorated stink bug Halyomorpha halys

FEBS Open Bio, EarlyView.
The brown marmorated stink bug, Halyomorpha halys, mainly secretes 4‐oxo‐(E)‐2‐hexenal, (E)‐2‐decenal, dodecane and tridecane, a mixture representative of that released by many heteropterans. We demonstrated that H. halys can synthesise these secretory components de novo using dietary glucose.
Haruna Fujimori, Koji Noge
wiley   +1 more source