Results 211 to 220 of about 444,200 (304)

Single‐molecule DNA flow‐stretch assays for high‐throughput DNA–protein interaction studies

FEBS Open Bio, EarlyView.
We describe an optimised single‐molecule DNA flow‐stretch assay that visualises DNA–protein interactions in real time. Linear DNA fragments are tethered to a surface and stretched by buffer flow for fluorescence imaging. Using λ and φX174 DNA, this protocol enhances reproducibility and accessibility, providing a versatile approach for studying diverse ...
Ayush Kumar Ganguli, Mohammad Nour Alsamsam, Ugnė Bagdonaitė, Van Truc Vu, Chun‐Jen Huang, Polina Kuzhir, Mindaugas Zaremba, Aurimas Kopūstas, Marijonas Tutkus   +8 more
wiley   +1 more source

Order from entropy: big data from FAIR data cohorts in the digital age of plant breeding. [PDF]

Theor Appl Genet
Gogna A, Arend D, Beier S, Rezaei EE, Würschum T, Zhao Y, Chu J, Reif JC.   +7 more
europepmc   +1 more source

Enabling FAIR data in Earth and environmental science with community-centric (meta)data reporting formats. [PDF]

Sci Data, 2022
Crystal-Ornelas R, Varadharajan C, O'Ryan D, Beilsmith K, Bond-Lamberty B, Boye K, Burrus M, Cholia S, Christianson DS, Crow M, Damerow J, Ely KS, Goldman AE, Heinz SL, Hendrix VC, Kakalia Z, Mathes K, O'Brien F, Pennington SC, Robles E, Rogers A, Simmonds M, Velliquette T, Weisenhorn P, Welch JN, Whitenack K, Agarwal DA.   +26 more
europepmc   +1 more source

Screening and epitope characterization of Nidogen‐2‐specific nanobodies

FEBS Open Bio, EarlyView.
Camel immunization and phage display were employed to generate high‐affinity VHH nanobodies against Nidogen‐2. After library construction, biopanning, ELISA screening, sequencing, and recombinant expression, selected nanobodies were purified and characterized, leading to the preliminary exploration of a nanobody‐based sandwich ELISA for specific ...
Jianchuan Wen, Qianqian Cui, Zhongyun Lan, Yingjun Wang, Shuaiying Zhao, Wenxuan Feng, Yunfeng Liu, Qiting Huang, Dongna Zhang, Jianfeng Xu   +9 more
wiley   +1 more source

FAIR data - the photon and neutron communities move together towards open science. [PDF]

IUCrJ
Murphy BM, Götz A, Gutt C, McGuinness C, Rønnow HM, Schneidewind A, Deledda S, Pietsch U.   +7 more
europepmc   +1 more source

YlmG1 is localized exclusively to the chloroplast envelope membrane and is involved in preprotein translocation in Arabidopsis thaliana

FEBS Open Bio, EarlyView.
Cytosolically synthesized chloroplast preproteins are translocated across the outer and inner envelope membranes through translocons called TOC and TIC, respectively. In green algae and plants, the TIC core is composed of essential membrane proteins, Tic12, Tic20, and Tic214.
Mengyi Li, Xueyang Zhao, Masato Nakai
wiley   +1 more source

An ecosystem for producing and sharing metadata within the web of FAIR Data. [PDF]

Gigascience
Jacob D, Ehrenmann F, David R, Tran J, Mirande-Ney C, Chaumeil P.   +5 more
europepmc   +1 more source

Moving Towards FAIR Data Practices in Pharmacy Education. [PDF]

Am J Pharm Educ, 2022
McLaughlin JE, Tropsha A, Nicolazzo JA, Crescenzi A, Brouwer KL.   +4 more
europepmc   +1 more source

Iron homeostasis disruption and lipid peroxidation in skeletal muscle during short‐term immobilization

FEBS Open Bio, EarlyView.
14‐day casting‐induced immobilization reduced gastrocnemius muscle mass and increased non‐heme iron and ferritin heavy chain levels. Despite iron accumulation, transferrin receptor 1 and iron regulatory protein 2 were paradoxically upregulated. Lipid peroxidation was elevated without compensatory antioxidant responses.
Haruka Yokogawa, Kazuhiko Higashida, Naoya Nakai   +2 more
wiley   +1 more source

A practical guide to FAIR data management in the age of multi-OMICS and AI. [PDF]

Front Immunol
Mugahid D, Lyon J, Demurjian C, Eolin N, Whittaker C, Godek M, Lauffenburger D, Fortune S, Levine S.   +8 more
europepmc   +1 more source