Results 111 to 120 of about 52,035 (209)

Substrate stiffness regulates cell fate and its applications in the repair of motor system: From two dimensions to three dimensions

BMEMat, EarlyView.
Substrate stiffness with different dimensions (2D and 3D) exerts a regulatory role in cellular behaviors, thereby governing cell fate decisions. Biomaterials with tunable stiffness exhibit substantial functional potential for the repair and regeneration of the motor system.
Zhiyuan Lu, Renwang Sheng, Ludvig J. Backman, Chuanquan Liu, Danning Zheng, Wei Zhang, Jialin Chen   +6 more
wiley   +1 more source

Nanotopographical cues mechanically regulate the secretion and osteoinductive potential of MSC‐derived exosomes for bone regeneration

BMEMat, EarlyView.
A defined 700 nm low‐density nanotopographical interface mechanically primes MSCs through the integrin β1–focal adhesion–Akt–Rab27a‐dependent pathway, enhancing exosome secretion and osteoinductive potency. Enrichment of miR‐548d‐5p in these exosomes promotes osteogenic differentiation and bone regeneration, establishing nanotopography‐guided MSC ...
Ziqi Zhang, Limeng Zheng, Zehui Xu, Xiaoyang Han, Guangli Xiang, Yizeng Chang, Hong Chen, Mo Yang, Wai Yin Cheng, Nathanael Chun Him Lai, Weihao Yuan, Siu Hong Dexter Wong, Bohan Yin   +12 more
wiley   +1 more source

LncRNA FAISL Inhibits Calpain 2‐Mediated Proteolysis of FAK to Promote Progression and Metastasis of Triple Negative Breast Cancer

Advanced Science
Triple negative breast cancer (TNBC) is the most aggressive subtype in breast tumors. When re‐analyzing TCGA breast cancer dataset, we found cell adhesion molecules are highly enriched in differentially expressed genes in TNBC samples, among which Focal ...
Yunmei Zhang, Shiyu Wei, Zhengjie Chen, Rui Xu, Shu‐Rong Li, Lili You, Ruiyue Wu, Yin Zhang, Jian‐You Liao, Xiaoding Xu, Erwei Song, Man‐Li Luo   +11 more
doaj   +1 more source

FAK-Del33 is hyper-phosphorylated in a cell-line dependent fashion.

, 2014
(A) Lysates containing 1 mg of total cellular protein were prepared from HA-tagged FAK-WT (lanes 1-2) and FAK-Del33 stable transfected cells (lanes 3-4). The cells overexpressing FAK were grown in the absence (induction of expression, even-numbered lanes)
Xiangfan Liu (294313), Peihua Ni (294310), Qishi Fan (294315), Ling Yao (16961), Changqiang Chen (294311), Xuqian Fang (630321), Jiafei Lin (630322), Xinmin Zheng (401187)   +7 more
core   +1 more source

Talin1 Adhesion Morphology and Colocalization With Tensin3 Are Largely Unaffected by Polyacrylamide Substrate Stiffness

Cytoskeleton, EarlyView.
ABSTRACT Cells sense the stiffness of their extracellular matrix (ECM) and adapt their behavior accordingly. We investigated how ECM stiffness affects the spatial organization of talin1, a key mechanosensitive focal adhesion protein. Using polyacrylamide (PA) hydrogels with tunable stiffnesses (0.2–188 kPa), we analyzed cell morphology, migration ...
Joanna Hajduk, Patrycja Twardawa, Sylwia Bobis‐Wozowicz, Marta Szewczyk, Zenon Rajfur, Zbigniew Baster   +5 more
wiley   +1 more source

On the relationship of YAP and FAK in hMSCs and osteosarcoma cells: discrimination of FAK modulation by nuclear YAP depletion or YAP silencing

, 2019
The HIPPO pathway effector YAP has been shown to be regulated by FAK-signaling. However, the existence of an inverse relationship between YAP and FAK is unknown.
Jung, Britta Antonia, Husari, Ayman, Dieterle, Martin P., Steinberg, Thorsten, Rühe, Jürgen, Prucker, Oswald, Tomakidi, Pascal   +6 more
core   +1 more source

Cytoskeleton–Membrane Uncoupling in Duchenne Muscular Dystrophy: Implications for Newborn Screening and Early Protection

Cytoskeleton, EarlyView.
ABSTRACT The cytoskeleton of striated muscle integrates force transmission, mechanotransduction, and sarcolemmal stability through coordinated networks of sarcomeres, costameres, and intermediate filaments. Together, these systems establish mechanical continuity between the contractile apparatus, the sarcolemma, and the extracellular matrix.
Houda Cohen, Nora Hosny, Brian R. Thompson, Joseph M. Metzger   +3 more
wiley   +1 more source

Roles and inhibitors of FAK in cancer: current advances and future directions

Frontiers in Pharmacology
Focal adhesion kinase (FAK) is a non-receptor tyrosine kinase that exhibits high expression in various tumors and is associated with a poor prognosis. FAK activation promotes tumor growth, invasion, metastasis, and angiogenesis via both kinase-dependent ...
Hui-Hui Hu, Sai-Qi Wang, Sai-Qi Wang, Hai-Li Shang, Hui-Fang Lv, Bei-Bei Chen, Bei-Bei Chen, She-Gan Gao, Xiao-Bing Chen, Xiao-Bing Chen   +9 more
doaj   +1 more source

Y397E FAK demonstrates residual activity.

, 2018
(A) Peptide pulldowns using bead-immobilized FERM-kinase linker domain peptides and recombinant SRC-GST. The pulldown blot indicates which samples captured SRC-GST, while the input blot indicates the amount of SRC-GST in each sample prior to ...
Sindhuja Sominidi-Damodaran (5509265), Cera A. McDonald (5509268), Edwin J. Squirewell (5509271), Saranya P. Wyles (5509277), Ming Li (91180), Alexander Meves (5509280), Ralph T. Böttcher (263397), Joel B. Heim (300828), Jan van Deursen (346359), Catherine Motsonelidze (5509274)   +9 more
core   +1 more source

Increased Insulin Action, Glucose Metabolism and Muscle Function in Supervillin‐Knockout and Supervillin‐Mutant Mice

Cytoskeleton, EarlyView.
ABSTRACT We here describe mouse models with complementary homozygous Svil mutations. In skeletal muscle, Svil‐Mut mice express the Svil‐encoded N‐terminus fused to the βgal‐neo gene‐trap tag and lack the highly conserved archvillin C‐terminus; Svil‐KO mice lack expression of all known Svil‐encoded proteins; and Svil‐LoxP mice contain loxP sites for ...
Tara C. Smith, Lauren A. Tauer, Randall H. Friedline, Xiaodi Hu, Duy Tran, Yangyi E. Luo, Nacima Hadjout‐Rabi, Elisabeth R. Barton, Jason K. Kim, Elizabeth J. Luna   +9 more
wiley   +1 more source