Results 91 to 100 of about 535,411 (309)

Single‐molecule DNA flow‐stretch assays for high‐throughput DNA–protein interaction studies

FEBS Open Bio, EarlyView.
We describe an optimised single‐molecule DNA flow‐stretch assay that visualises DNA–protein interactions in real time. Linear DNA fragments are tethered to a surface and stretched by buffer flow for fluorescence imaging. Using λ and φX174 DNA, this protocol enhances reproducibility and accessibility, providing a versatile approach for studying diverse ...
Ayush Kumar Ganguli, Mohammad Nour Alsamsam, Ugnė Bagdonaitė, Van Truc Vu, Chun‐Jen Huang, Polina Kuzhir, Mindaugas Zaremba, Aurimas Kopūstas, Marijonas Tutkus   +8 more
wiley   +1 more source

Time‐restricted feeding prior to Mycobacterium tuberculosis infection reduces tissue CD4+ T cells with limited impact on bacterial clearance

FEBS Open Bio, EarlyView.
Time‐restricted feeding (TRF) in mice increased liver fatty acid oxidation and decreased fatty acid biosynthesis. These alterations persisted when TRF was discontinued and the host was infected with Mycobacterium tuberculosis. Pre‐exposure to TRF did not alter tissue (lung and spleen) mycobacterial burden but significantly reduced CD3+ T cells in lungs
Ashish Gupta, Nidhi Yadav, Subhasmita Das, R. Rajendra Kumar Reddy, Nupur Sharma, Amol Ratnakar Suryawanshi, Jaswinder Singh Maras, Ranjan Kumar Nanda   +7 more
wiley   +1 more source

Lipase inhibition attenuates the acute inhibitory effects of oral fat on food intake in healthy subjects

, 2003
The lipase inhibitor, orlistat, is used in the treatment of obesity and reduces fat absorption by about 30%. However, the mean weight loss induced by orlistat is less than expected for the degree of fat malabsorption.
O'Donovan, D., Wishart, J., Horowitz, M., Feinle-Bisset, C.   +3 more
core   +1 more source

Creating an acute energy deficit without stimulating compensatory increases in appetite: is there an optimal exercise protocol? [PDF]

, 2014
Recent years have witnessed significant interest from both the scientific community and the media regarding the influence of exercise on subsequent appetite and energy intake responses.
Stensel, DJ, Deighton, K, Kevin Deighton, David J. Stensel   +3 more
core   +1 more source

Comparing weight loss rates between males and females: a preliminary analysis

Journal of the International Society of Sports Nutrition
Background Biological differences between males and females include variations in fat-free mass, resting metabolic rate, and hormone profiles. Males have more lean muscle and visceral fat, likely associated with differences in testosterone, while females
Valentina Rodriguez Da Silva, Ryan Singh, Valeria V. Silva Ramirez, Daniel Arevalo, Eric T. Trexler, Landon Shannahan, John Solis, Samuel Blanke, Samuel Evers, Talon Westervelt, John Holtje, Bill I. Campbell   +11 more
doaj   +1 more source

Effects of IGFBP4 deficiency on human preadipocyte proliferation and differentiation through the IGF1R/AKT pathway

FEBS Open Bio, EarlyView.
IGFBP4 knockdown (KD) impairs preadipocyte proliferation and is associated with IGF1R protein downregulation and attenuated AKT phosphorylation. The mechanisms by which IGFBP4 KD influences the IGF1R/AKT signaling pathway involve newly synthesized proteins and lysosomal degradation pathways. Created in BioRender.
Yujia Guo, Sandy Richter, Christoph Bach, Wieland Kiess, Anna Kirstein, Diana Le Duc, Antje Garten   +6 more
wiley   +1 more source

Investigating Web Search Strategies and Forum Use to Support Diet and Weight Loss [PDF]

, 2009
Healthcare is shifting from being reactive to preventive, with a focus on maintaining general wellness through positive decisions on diet, exercise, and lifestyle.
André, Paul, Ryen W. White, Tan, Desney, m.c. schraefel, Paul André, Desney Tan, schraefel, m.c., White, Ryen W.   +7 more
core   +1 more source

From energy provision to protein synthesis: Tunnelling nanotubes as mediators of intercellular metabolic cooperation in cancer

FEBS Open Bio, EarlyView.
The cytoskeleton‐mediated transport of mitochondria via tunnelling nanotubes restores respiration, increases ATP production, rescues cells from apoptosis, activates the AKT/mTOR signalling pathway, promotes cell migration and invasiveness, contributes to cancer progression and treatment resistance.
Stanislava Martínková, Jan Trnka
wiley   +1 more source

Loss-of-function miRNA heterozygotes altered body fat.

, 2019
MiRNA knock-out (KO) lines were mated with the Act5C-Gal4 driver. The resulting F1 heterozygotes were comparatively analyzed against control flies that were generated from the cross between W1118 and the same Act5C-Gal4 driver.
Shuyin Zhang (6997076), Jin Seo (289425), Morgan Youngblood (6997079), Russell Arellanes (6997067), Jared Yeahquo (6997073), Dylan Allen (6997061), Lucille Redmond (6997070), Austin Reiner (6997082), M. Christian Elledge (6997064), William Redmond (6997058)   +9 more
core   +1 more source

High-Fat Diet : Bacteria Interactions Promote Intestinal Inflammation Which Precedes and Correlates with Obesity and Insulin Resistance in Mouse [PDF]

, 2010
Background: Obesity induced by high fat (HF) diet is associated with inflammation which contributes to development of insulin resistance. Most prior studies have focused on adipose tissue as the source of obesity-associated inflammation.
Michael M Chi, Scott Magness, Jobin, Christian, Christian Jobin, Jobin Christian, Scull Brooks P., Brooks P. Scull, Lund Pauline K., Chi Michael M., Magness Scott, Shengli Ding, Pauline K Lund, Scull, Brooks P., Nicole M. J. Schwerbrock, Brooks P Scull, Nicole M J Schwerbrock, Chi, Michael M., Ding Shengli, Magness, Scott, Rigby Rachael, Schwerbrock, Nicole M. J., Schwerbrock Nicole M. J., Rigby, Rachael, Ding, Shengli, Lund, Pauline K., Michael M. Chi, Pauline K. Lund, Rachael Rigby   +27 more
core   +1 more source