Results 71 to 80 of about 14,064 (255)

Identifying and characterising a plant GH1 β‐glucosidase that exhibits hydrolytic activity on N‐linked glucopyranoside

open access: yesFEBS Open Bio, EarlyView.
We report the first β‐glucosidase with demonstrated hydrolytic activity on an N‐linked glycopyranoside. The enzyme, native to maize, was biochemically characterised for this novel reaction, and structural modelling of the enzyme–substrate complex revealed several clues to the underlying reduced catalytic rate relative to its native O‐glycopyranoside ...
Hani Gharabli   +3 more
wiley   +1 more source

Method for Resilience-oriented Optimization and Evaluation of Distribution Networks Considering Load Restoration Prioritization and Dynamic Repair Under Typhoon Disasters [PDF]

open access: yesDianli jianshe
[Objective] To enhance the resilience of distribution networks under typhoon disasters and mitigate the risks of load interruptions and power supply losses caused by natural disasters, this paper proposes a resilience-oriented optimization strategy and ...
YAN Renwu, GUO Yumin, LI Peiqiang
doaj   +1 more source

Purification and preparation of Marchantia polymorpha Auxin Response Factor 2 for phase separation studies

open access: yesFEBS Open Bio, EarlyView.
We describe detailed protocols for the purification and preparation of Marchantia polymorpha Auxin Response Factor 2 (MpARF2). This protein is fused to an MBP solubility tag and an mNG fluorescent tag and is purified from Escherichia coli. The presented procedures make it possible to study MpARF2 assemblies, which could arise from phase separation ...
Bas Janssen   +5 more
wiley   +1 more source

Protocol for quantifying miRNA trafficking across the endosomal membrane

open access: yesFEBS Open Bio, EarlyView.
An in vitro protocol measures miRNA uptake into endosomes isolated from mammalian cell extracts, which are free of subcellular contaminants. Performed at 37 °C in the presence of ATP, it ensures the import of single‐stranded miRNA into the endosomal lumen.
Syamantak Ghosh   +2 more
wiley   +1 more source

PERANCANGAN SISTEM PAKAR UNTUK IDENTIFIKASI DAN REKOMENDASI PERBAIKAN KERUSAKAN PADA MOTOR AC

open access: yesTeknologi dan Kejuruan, 2012
: The design of expert system for identifying and recommending fault recovery of AC motor. The existing vibration measurement instruments do not have capability to identify AC motor fault and give the information about the cause of it and reparation ...
Totok Soehartanto, Arif Fajar Solikin
doaj   +1 more source

Sodium Outdiffusion from Stacking Faults as Root Cause for the Recovery Process of Potential-induced Degradation (PID)

open access: yesEnergy Procedia, 2014
AbstractPotential-induced degradation (PID) is currently one of the most important and prominent module degradation mechanisms leading to significant yield losses. It was shown that Na decorated stacking faults are responsible for this degradation mechanism of the solar modules.
Lausch, Dominik   +6 more
openaire   +1 more source

Structural and biochemical insights into the thermostable esterase Ta0887 from Thermoplasma acidophilum

open access: yesFEBS Open Bio, EarlyView.
In this study, a novel esterase from the thermoacidophilic archaeon Thermoplasma acidophilum was biochemically and structurally characterized. Our results demonstrate that Ta0887 is a highly thermostable esterase that preferentially hydrolyzes p‐nitrophenyl hexanoate and possesses an α‐helical cap domain that likely contributes to its substrate ...
Alejandro Delgado‐Rey   +4 more
wiley   +1 more source

Acoustic Emission Characteristics of Fault Activation under the Influence of Coal Roadway Driving

open access: yesShock and Vibration
To investigate the impact of coal roadway excavation on the fault activity, this study examined the acoustic emission (AE) count, energy characteristics, and evolution of the AE b-value during the tunneling process across a fault by utilizing field data ...
Pengbo Wang   +4 more
doaj   +1 more source

Semi-Supervised and Disentangled Causal Discovery for Analyzing Fault Propagation in Microservices

open access: yesIEEE Access
While troubleshooting fault propagation in a microservice-based system is labor-intensive for operators, it is critical to their decision-making process for maintaining availability and reliability.
Nobukazu Fukuda   +5 more
doaj   +1 more source

Risk Assessment Method of UHV AC/DC Power System under Serious Disasters

open access: yesEnergies, 2016
Based on the theory of risk assessment, the risk assessment method for an ultra-high voltage (UHV) AC/DC hybrid power system under severe disaster is studied.
Rishang Long, Jianhua Zhang
doaj   +1 more source

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