Results 101 to 110 of about 809,365 (289)

Clinical performance of the urine‐based TERT promoter AbsoluteQ Digital PCR for non‐invasive detection of bladder cancer

Molecular Oncology, EarlyView.
A urine‐based digital PCR assay targeting two hotspot TERT promoter variants detected bladder cancer with high sensitivity and no false positives in this case–control cohort. The streamlined AbsoluteQ workflow outperformed Sanger sequencing and supports non‐invasive molecular testing for bladder cancer detection.
Anna Nykel, Izabela Kubiak, Lena Rutkowska, Żaneta Kasprzyk, Łukasz Kępczyński, Michał Bednarek, Dariusz Sobieraj, Jacek Wilkosz, Piotr Kania, Adam Jędrzejczyk, Bogdan Kałużewski, Agnieszka Gach, Tadeusz Kałużewski   +12 more
wiley   +1 more source

Automated FRAP microscopy for high‐throughput analysis of protein dynamics in chromatin organization and transcription

FEBS Open Bio, EarlyView.
RoboMic is an automated confocal microscopy pipeline for high‐throughput functional imaging in living cells. Demonstrated with fluorescence recovery after photobleaching (FRAP), it integrates AI‐driven nuclear segmentation, ROI selection, bleaching, and analysis.
Selçuk Yavuz, Bart Geverts, Johan A. Slotman, Andrea Sacchetti, Stefan Prekovic, Martin E. van Royen, Adriaan B. Houtsmuller   +6 more
wiley   +1 more source

A Steepest Feasible Direction Method for Linear Programming. Derivation and Embedding In the Simplex Method [PDF]

Operations Research and Decisions
A feasible direction method for linear programming has been proposed. The method is embedded in the framework of the simplex method, even though it works with non-edge feasible directions.
Biressaw C. Wolde, Torbjörn Larsson
doaj  

A Survey on Automated Pain Recognition and Assessment Using Multimodal

Cybernetics and Information Technologies
Practical pain assessment is essential in clinical practice. However, conventional methods for pain assessment depend primarily on patients’ self-reports, which are not feasible for non-communicative individuals, such as neonates and unconscious patients.
Jasim Wala’a N., Chyad Adala Mahdi
doaj   +1 more source

Raman‐based label‐free microscopic analysis of the pancreas in living zebrafish larvae

FEBS Open Bio, EarlyView.
Forward stimulated Raman scattering (F‐SRS) and epi coherent anti‐Stokes Raman scattering (E‐CARS) allow label‐free discrimination of distinct subcellular structures in the pancreas of living zebrafish larvae. Given the straightforward applicability, we anticipate broad implementation of Raman microscopy in other organs and across various biomedical ...
Noura Faraj, Eline M. F. de Lange, Klaas A. Sjollema, Ben N. G. Giepmans   +3 more
wiley   +1 more source

Surface functionalization strategies for polydiacetylene-based colorimetric sensors

Communications Chemistry
Polydiacetylene (PDA)-based colorimetric sensors offer a promising platform for rapid and visual detection, through a chromatic transition from blue to red.
Brainy Happy Ana Tasiman, Rizky Aflaha, Wiyogo Prio Wicaksono, Ganjar Fadillah, Yuliyan Dwi Prabowo, Joan Daniel Prades, Erwin Peiner, Kuwat Triyana, Hutomo Suryo Wasisto   +8 more
doaj   +1 more source

Advances in Organocatalyzed Atom Transfer Radical Polymerization

Advances in Polymer Technology, 2019
Atom transfer radical polymerization (ATRP) is one of the most robust tools to prepare well-defined polymers with precise topologies and architectures. Although series of improved ATRP methods have been developed to decrease the metal catalyst loading to
Xin Hu, Ning Zhu, Kai Guo
doaj   +1 more source

Optimizing for minimum weight when two different finite element models and analyses are required [PDF]

The Finite Element Structural Optimization Program's (FESOP) ability to perform minimum weight optimization using two different finite element analyses and models is discussed. FESOP uses the ADS optimizer developed by Dr.
Hall, Jeffrey C.
core   +1 more source

Multi‐omics and low‐input proteomics profiling reveals dynamic regulation driving pluripotency initiation in early mouse embryos

FEBS Open Bio, EarlyView.
Mouse pre‐implantation development involves a transition from totipotency to pluripotency. Integrating transcriptomics, epigenetic profiling, low‐input proteomics and functional assays, we show that eight‐cell embryos retain residual totipotency features, whereas cytoskeletal remodeling regulated by the ubiquitin‐proteasome system drives progression ...
Wanqiong Li, Xi Xiao, Lingbin Qi, Chanyi Li, Sirui Song, Jiaying Qin, Zhigang Xue, Jinfeng Xue, Huaifang Li   +8 more
wiley   +1 more source

Meta‐analysis fails to show any correlation between protein abundance and ubiquitination changes

FEBS Open Bio, EarlyView.
We analyzed over 50 published proteomics datasets to explore the relationship between protein levels and ubiquitination changes across multiple experimental conditions and biological systems. Although ubiquitination is often associated with protein degradation, our analysis shows that changes in ubiquitination do not globally correlate with changes in ...
Nerea Osinalde, Unai Alduntzin, Gorka Prieto, Ugo Mayor   +3 more
wiley   +1 more source