Results 41 to 50 of about 74,171 (258)

A serum substitute for fed-batch culturing of hybridoma cells [PDF]

Cytotechnology, 2008
We developed a substitute for serum to produce fed-batch cultures of hybridoma cells in serum-free medium and confirmed that the cells could be successfully cultivated this way. Our substitute consisted of 12 components. The specific production rates of lactate and ammonia, which are harmful byproducts from the cells, were significantly reduced ...
Keisuke, Shibuya, Ryoichi, Haga, Masaru, Namba   +2 more
openaire   +2 more sources

Simulation of kefiran production of Lactobacillus kefiranofaciens JCM6985 in fed-batch reactor [PDF]

Songklanakarin Journal of Science and Technology (SJST), 2006
Kinetics of kefiran production by Lactobacillus kefiranofaciens JCM6985 has been investigated. A mathematical model taking into account the mechanism of exopolysaccharides production has been developed. Experiments were carried out in batch mode in order
Benjamas Cheirsilp
doaj  

Innovative Processing of Compacted Waste Aluminum Alloy Powders via Controlled Remelting and Solidification

Advanced Engineering Materials, EarlyView.
This study demonstrates an efficient recycling route for out‐of‐spec AlSi10Mg atomized powders through compaction and arc remelting followed by suction casting. By correlating compaction load, cooling rate, and resulting microstructure, we show that intermediate pressures (50–80 kN) and rapid cooling refine dendrites, reduce porosity, and enhance ...
Mila Christy de Oliveira, Marcella Gaute Cavalcante Xavier, Abdoul‐Aziz Bogno, Piter Gargarella, José Eduardo Spinelli   +4 more
wiley   +1 more source

Production of 1,3-Propanediol by Clostridium Butyricum Growing on Biodiesel Derived Glycerol

Chemical Engineering Transactions, 2016
The continuous demand for alternative biofuels resulted in a significant rise of biodiesel production in last decade. As a consequence, high quantities of raw glycerol have been accumulated.
F. Martins, V. Saab, C. Ribeiro, M.A. Coelho, T. Ferreira   +4 more
doaj   +1 more source

Cell factory for γ-aminobutyric acid (GABA) production using Bifidobacterium adolescentis

Microbial Cell Factories, 2022
Background Bifidobacteria are gram-positive, probiotic, and generally regarded as safe bacteria. Techniques such as transformation, gene knockout, and heterologous gene expression have been established for Bifidobacterium, indicating that this bacterium ...
Hend Altaib, Tomoya Kozakai, Yassien Badr, Hazuki Nakao, Mahmoud A. M. El-Nouby, Emiko Yanase, Izumi Nomura, Tohru Suzuki   +7 more
doaj   +1 more source

Influence of an Oxygen‐Free Atmosphere on Diamond‐Single‐Grain Scratching of Ti–6Al–4V

Advanced Engineering Materials, EarlyView.
Single‐grain scratching of Ti–6Al–4V is investigated under controlled, oxygen‐free, and ambient atmospheres using a novel experimental setup with in situ high‐speed imaging. The approach enables direct observation of chip formation and adhesion under suppressed oxidation.
Berend Denkena, Benjamin Bergmann, Michael Zenger   +2 more
wiley   +1 more source

Kinetic study of batch and fed-batch enzymatic saccharification of pretreated substrate and subsequent fermentation to ethanol

Biotechnology for Biofuels, 2012
Background Enzymatic hydrolysis, the rate limiting step in the process development for biofuel, is always hampered by its low sugar concentration. High solid enzymatic saccharification could solve this problem but has several other drawbacks such as low ...
Gupta Rishi, Kumar Sanjay, Gomes James, Kuhad Ramesh   +3 more
doaj   +1 more source

Techno-economic analysis of Cellulase Production by Trichoderma reesei in Submerged Fermentation Processes using a Process Simulator

South African Journal of Chemical Engineering, 2022
Enzymes are valuable in many biofuels production processes, and their wide application has generated significant market demand in recent years. To develop lignocellulosic fuels in a biorefinery, high cost and low catalytic efficiency of cellulase are a ...
Abiola Ezekiel Taiwo, Andykan Tom-James, Olayomi Abiodun Falowo, Anthony Okoji, Oladayo Adeyi, Abayomi O. Olalere, Andrew Eloka-Eboka   +6 more
doaj   +1 more source

Influence of Test Temperature and Test Frequency on Fatigue Life of Aluminum Alloy EN AW‐2618A

Advanced Engineering Materials, EarlyView.
The influence of test temperature and test frequency on the fatigue life of EN AW‐2618A is investigated. High‐cycle fatigue tests are performed at different test temperatures and frequencies on the 1000 h/230°C overaged state. Both test parameters reduce fatigue life due to time‐dependent damage mechanisms.
Ying Han, Julian M. Rosalie, Jan Radners, Mauro Madia, Philipp von Hartrott, Birgit Skrotzki   +5 more
wiley   +1 more source

Direct Metal Deposition of Graphene–Ti28Nb35.4Zr Matrix Composites With Enhanced Mechanical, Corrosion, and Biocompatibility Properties for Bone Implants

Advanced Engineering Materials, EarlyView.
Graphene nanoplatelet (0.1 wt.%) reinforcement significantly enhances the performance of β Ti‐28Nb‐35.4Zr alloy. Grain refinement, reduced water contact angle, and improved surface characteristics promote osteoblast adhesion and complete surface coverage after 7 days.
Khurram Munir, Jixing Lin, Yuncang Li, Li Zhu, Paul F. A. Wright, Cuie Wen   +5 more
wiley   +1 more source