Results 211 to 220 of about 11,099 (258)
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Fed-Batch Cultivation of Methanobacterium formicicum and its Fluorometric Monitoring
Acta Biotechnologica, 1989AbstractFed‐batch cultivation of Methanobacterium formicicum Z‐281 was performed under substrate limiting conditions. Specific rate of culture growth amounted to 0.041 h−1, cell yield related to substrate was 0.6 mg proteine/mM formate. Significant increase of coenzyme F420 content in cells depending on time of cultivation was observed. Increase of the
P. Kuschk, V. V. Kevbrin
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Fed-batch cultivation of Bordetella pertussis: Metabolism and Pertussis Toxin production
Biologicals, 2006The production of acellular pertussis in comparison with whole cell pertussis vaccines demands 5-25 times the amount of Bordetella pertussis' virulence factors, such as Pertussis Toxin (PT), to produce the same number of vaccine doses. An increase in the volumetric productivity by employing fed-batch rather than the currently used batch cultivations of
Thalen, M. +8 more
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Probing control of fed-batch cultivations: analysis and tuning
Control Engineering Practice, 2001Production of various proteins can today be made using genetically modified Escherichia coli bacteria. In cultivations of E. coli it is important to avoid accumulation of the by-product acetate. Formation of acetate occurs when the specific glucose uptake exceeds a critical value and can be avoided by a proper feeding strategy. A difficulty is that the
M. Åkesson, P. Hagander, J.P. Axelsson
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A fed‐batch cultivation method for the simultaneous utilization of glucose and formate by yeasts
Acta Biotechnologica, 1988AbstractA fed‐batch cultivation method was elaborated which 1. warrants simultaneous consumption of glucose and formate, 2. is easy to employ and provides reproducible results, 3. gives information about the role of formate in yeast growth, 4. results in almost identical conditions for parallel cultures on glucose and glucose/formate.
R. H. Müller, E. Zimmermann, W. Babel
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Optimal fed‐batch cultivation when mass transfer becomes limiting
Biotechnology and Bioengineering, 2007AbstractIn the design of an aerobic fed‐batch process to produce, for example, a pharmaceutical protein, the volumetric production rate will eventually become limited by mass transfer when the biomass concentration exceeds a certain upper limit x*. It appears to be common practice to switch from exponential feed of substrate to a constant feed rate ...
John, Villadsen, Kiran R, Patil
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2013
Fed-batch reactor is based on feeding of a growth limiting nutrient substrate to a culture. Cell growth and fermentation can be controlled by the feeding strategy. The fed-batch strategy is typically used to reach a high cell density in the bioreactor or mimic a continuous culture. Mostly the feed solution is highly concentrated.
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Fed-batch reactor is based on feeding of a growth limiting nutrient substrate to a culture. Cell growth and fermentation can be controlled by the feeding strategy. The fed-batch strategy is typically used to reach a high cell density in the bioreactor or mimic a continuous culture. Mostly the feed solution is highly concentrated.
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Assessment of fed-batch cultivation strategies for an inducible CHO cell line
Journal of Biotechnology, 2019In order to maximize cell growth and productivity for an inducible CHO cell line expressing rituximab, various fed-batch culture strategies were investigated. In each case, the performance was evaluated for cultures induced at moderate and high cell density conditions (4 × 106 and 10 × 106 cells/mL) to assess the impact of the timing of induction.
Kahina Mellahi +6 more
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Clavulanic Acid Degradation in Streptomyces clavuligerus Fed‐Batch Cultivations
Biotechnology Progress, 2002Abstract Clavulanic acid (CA) is an important antibiotic that is produced by Streptomyces clavuligerus . CA is unstable and product degradation has turned out to have a major impact on product titers in fed‐batch cultivations.
Johannes A, Roubos +4 more
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Cultivation of Arthrospira (Spirulina) platensis by Fed-Batch Process
2012This chapter comments on fed-batch cultivation of Arthrospira platensis under different carbon and nitrogen sources, pH, temperature, light intensity, type of photobioreactor and typical parameters of the fed-batch process, such as feeding time, addition protocol and flow rate.
João C. M. Carvalho +3 more
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Change in hyphal morphology of Aspergillus oryzae during fed-batch cultivation
Applied Microbiology and Biotechnology, 2006Industrial enzymes are often produced by filamentous fungi in fed-batch cultivations. During cultivation, the different morphological forms displayed by the fungi have an impact on the overall production. The morphology of a recombinant lipase producing Aspergillus oryzae strain was investigated during fed-batch cultivations.
Martin B, Haack +3 more
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