Results 31 to 40 of about 11,099 (258)

Parallel substrate supply and pH stabilization for optimal screening of E. coli with the membrane-based fed-batch shake flask

Microbial Cell Factories, 2018
Background Screening in the fed-batch operation mode is essential for biological cultivations facing challenges as oxygen limitation, osmotic inhibition, catabolite repression, substrate inhibition or overflow metabolism.
P. Philip, D. Kern, J. Goldmanns, F. Seiler, A. Schulte, T. Habicher, J. Büchs   +6 more
doaj   +1 more source

Cross Kingdom Metabolic Engineering Paradigm Elevating Sustainable Protein Production

Advanced Science, EarlyView.
ABSTRACT Confronting the dual crisis of escalating global protein demand and unsustainable agriculture necessitates transformative solutions. Here, we pioneer evolutionary insights from maize nitrogen optimization via asparagine synthetase (ASNS) to rewire metabolism in Pichia pastoris.
Yuanyuan Du, Changyu Pi, Kai Hong, Le Gao, Xin Wu   +4 more
wiley   +1 more source

Novel approach of high cell density recombinant bioprocess development: Optimisation and scale-up from microlitre to pilot scales while maintaining the fed-batch cultivation mode of E. coli cultures

Microbial Cell Factories, 2010
Background Bioprocess development of recombinant proteins is time consuming and laborious as many factors influence the accumulation of the product in the soluble and active form.
Rimšeliene Renata, Kraft Mario, Horn Uwe, Šiurkus Juozas, Panula-Perälä Johanna, Neubauer Peter   +5 more
doaj   +1 more source

Polychip‐A High‐Throughput Droplet Microfluidics Platform for Interrogating Microbial Interactions

Advanced Science, EarlyView.
Polychip, a fully integrated droplet microfluidics platform, enables high‐throughput, single‐cell resolution screening of polymicrobial interactions. By seamlessly combining six automated microfluidics operations on a single chip, the system accelerates antimicrobial discovery by 11 to 14 times compared to traditional robotic methods.
Jeong Jae Han, Adrian Ryan Guzman, Aifen Zhou, Han Zhang, Rohit Gupte, Haemin Jung, Kaylee Delgado, Sini Skariah, Ali Sultan, Arul Jayaraman, Paul de Figueiredo, Arum Han   +11 more
wiley   +1 more source

WT1‐Targeted Oral Bifidobacterium longum Vaccine Enhances Checkpoint Blockade Efficacy in Pancreatic Cancer

Advanced Science, EarlyView.
Oral WT1‐targeted Bifidobacterium longum vaccine promotes WT1‐associated cellular immunity and enhances anti‐PD‐1/anti‐CTLA‐4 efficacy in pancreatic cancer. ABSTRACT Pancreatic ductal adenocarcinoma (PDAC) remains largely unresponsive to immune checkpoint inhibitors (ICI) due to its profoundly immunosuppressive tumor microenvironment.
Taiki Yamazaki, Aori Minami, Misako Hirota, Mirei Hirano, Ryota Sato, Atsuki Honma, Hideto Ueki, Toshiro Shirakawa   +7 more
wiley   +1 more source

Transferability of bioprocessing modes for recombinant protease production: from fed-batch to continuous cultivation with Bacillus licheniformis

BMC Biotechnology
Background Proteases are essential in various industries due to their unique substrate specificities and robustness in different operational conditions.
Stefan Kittler, Fabian Müller, Mohamed Elshazly, Georg Benjamin Wandrey, Tobias Klein, Andreas Daub, Oliver Spadiut, Julian Kopp   +7 more
doaj   +1 more source

Engineered Optogenetic Circuits In Yeast with Self‐Sustained Outputs

Advanced Science, EarlyView.
Optogenetic quorum‐sensing (OptoQS) circuit enables Saccharomyces cerevisiae to record transient light stimulus with self‐sustained outputs. Panel a indicates the design of the OptoQS circuit based on G‐protein coupled receptor (GPCR) signaling cascade. Panel b indicates the mode of action of surrogate messenger‐mediated signal transmission at the cell
Cong Fan, Haofeng Chen, Yan Wang, Junyi Wang, Yueyang Chen, Yang Zhang, Jinting Guan, Jifeng Yuan   +7 more
wiley   +1 more source

Multi‐Stimuli‐Responsive Hydrogen‐Bonded Organic Frameworks Nanocarriers Enable Targeted Fungicide Release and Plant Immune Regulation

Advanced Science, EarlyView.
A fluorinated hydrogen‐bonded organic framework nanocarrier (PFC‐1‐F) loaded with PYR and coated with HACC enables multi‐stimuli‐responsive (enzyme/ROS/pH) release at infection sites. Exhibiting targeted antifungal activity against Sclerotium rolfsii, modulating plant antioxidant defense, and demonstrating favorable biosafety across plant, soil, and ...
Guangming Ma, Ying Zou, Yao Wang, Huiyan Li, Jinlin Li, Tianfu Liu, Xiaohong Pan   +6 more
wiley   +1 more source

Enhancing Biomass Production and Biodesulfurization Efficiency of Rhodococcus qingshengii IGTS8: Evaluation of Batch, Repeated Batch and Fed-Batch Cultivation Techniques

Applied Sciences
The batch, repeated batch and fed-batch cultivation strategies, in stirred tank bioreactors, were evaluated to maximize biomass production and the cells’ desulfurization activity (CDA) of Rhodococcus qingshengii IGTS8.
Konstantinos Dimos, Styliani Kalantzi, George Prasoulas, Panagiotis D. Gklekas, Olga Martzoukou, Dimitris G. Hatzinikolaou, Dimitris Kekos, Diomi Mamma   +7 more
doaj   +1 more source

Implementation of Sliding Mode Controller with Boundary Layer for Saccharomyces cerevisiae Fed-batch Cultivation [PDF]

Bioautomation, 2005
An implementation of sliding mode control for yeast fed-batch cultivation is presented in this paper. Developed controller has been implemented on two real fed-batch cultivations of Saccharomyces cerevisiae.
Stoyan Tzonkov, Iasen Hristozov, Tania Pencheva, Dirk Huell, Christine Klockow, Bernd Hitzmann   +5 more
doaj