Results 261 to 270 of about 111,815 (303)

Enhanced sophorolipid production by feeding-rate-controlled fed-batch culture

Bioresource Technology, 2009
To develop the easier control method for fed-batch culture of sophorolipid production, we chose rapeseed oil as the most productive oil and compared their productivities in relation to different concentrations of glucose. The optimal concentration of glucose was 30 g/L for sophorolipid production.
Young-Bum, Kim, Hyun Shik, Yun, Eun-Ki, Kim   +2 more
openaire   +2 more sources

Control of endotoxin release in Escherichia coli fed-batch cultures

Bioprocess and Biosystems Engineering, 2004
High amounts of outer membrane (OM) components were released in glucose-limited fed-batch (GLFB) cultures at 37 degrees C at specific growth rates approaching 0.05 h(-1). Endotoxin analyses from a 20 degrees C GLFB culture gave similar results. An alternative fermentation technique, the temperature-limited fed-batch (TLFB) technique, reduced the ...
Marie, Svensson, Ling, Han, Gustav, Silfversparre, Lena, Häggström, Sven-Olof, Enfors   +4 more
openaire   +2 more sources

Defined protein and animal component-free NS0 fed-batch culture

Biotechnology and Bioengineering, 2007
A chemically defined protein and animal component-free fed-batch process for an NS0 cell line producing a human IgG(1) antibody has been developed. The fed-batch feed profile was optimised in a step-wise manner. Depletion of measurable compounds was determined by direct analysis.
Erika, Spens, Lena, Häggström
openaire   +2 more sources

Controlling glycation of recombinant antibody in fed‐batch cell cultures

Biotechnology and Bioengineering, 2011
AbstractProtein glycation is a non‐enzymatic glycosylation that can occur to proteins in the human body, and it is implicated in the pathogenesis of multiple chronic diseases. Glycation can also occur to recombinant antibodies during cell culture, which generates structural heterogeneity in the product. In a previous study, we discovered unusually high
Inn H, Yuk, Boyan, Zhang, Yi, Yang, George, Dutina, Kimberly D, Leach, Natarajan, Vijayasankaran, Amy Y, Shen, Dana C, Andersen, Bradley R, Snedecor, John C, Joly   +9 more
openaire   +2 more sources

Substrate level control in fed-batch hybridoma cultures

1994
Summary By controlling the substrate concentrations in fed-batch cultures yields of hybridoma cells and product can be improved. Possibilities for process control are determined by reproducibility of cell cultivation, quality of the process model, and the amount and quality of available measurement data.
B. Romein, P. van Londen, J.J. Meijer, C. Hellinga, J.P. van Dijken, K.Ch.A.M Luyben   +5 more
openaire   +1 more source

Optimization of a Fed-batch Culture by Statistical Data Analysis

IFAC Proceedings Volumes, 1983
Abstract An algorithm for the optimization of a fermentation process was investigated using the combination of dynamic programming and a linear predictive procedure by regression analysis. It was applied to the fed-batch culture for glutamic acid production with ethanol feeding and was proved to be effective for solving the optimization problems ...
M. Kishimoto, T. Sawano, T. Yoshida, H. Taguchi   +3 more
openaire   +1 more source

Comparison of Fed-batch Strategies in Hybridoma Cultures

2008
The classical cell culture process in stirred tank or airlift reactors is still the mostly used large scale production system. Fed-batch processes with different feeding strategies have been investigated to improve the productivity of hybridoma cultures.
J. O. Schwabe, Ines Wilkens, R. PÖrtner
openaire   +1 more source

Product Consistency During Long-Term Fed-Batch Culture

1994
Synopsis We have used a glutamine synthetase expression vector system1 to generate NSO myeloma cell lines expressing high levels of recombinant antibody genes. Medium depletion analysis has been used to design customized fed-batch processes which maintain the cells in viable condition for periods of three to four weeks and yield titers of nearly 1 g ...
D.K. Robinson, C.P. Chan, T.C. Seamans, D.K. Lee, A.B. Lenny, J.-S. Tung, D.J. DiStefano, S. Munshi, S.L. Gould, G.E. Mark, C.C. Yu Ip, P.K. Tsai, J. Irwin, M. Silberklang   +13 more
openaire   +1 more source

Profile control scheme in a Bakers' yeast fed‐batch culture

Biotechnology and Bioengineering, 1985
AbstractThis article develops and discusses a practical and useful computer control scheme so that the biomass concentration or the specific growth rate will as accurately as possible follow a desired profile specified in advance. Many computer simulations certified the validity of the proposed control scheme.
T, Takamatsu, S, Shioya, Y, Okada, M, Kanda   +3 more
openaire   +2 more sources

Optimisation of fed-batch culture of hybridoma cells using genetic algorithms

ISA Transactions, 2001
In this paper, a program describing a genetic algorithm is used for optimising fed-batch culture hybridoma cells to obtain the highest yield over certain time period. Optimal feed rate trajectories for a single feed stream containing both glucose and glutamine, and separate feed streams of glucose and glutamine are determined via the genetic algorithm.
S K, Nguang, L, Chen, X D, Chen
openaire   +2 more sources