Results 251 to 260 of about 53,182 (300)

Metabolically optimised BHK cell fed-batch cultures

Journal of Biotechnology, 2000
The aim of this work was the optimisation of a fed-batch culture by metabolic confinement of BHK21 cells producing an antibody/cytokine fusion protein with potential application in tumour-targeted therapy. Previous results showed that at very low nutrient concentrations, a metabolic shift towards more efficient metabolic pathways occurs.
H J, Cruz, J L, Moreira, M J, Carrondo
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Optimization of a Fed-batch Culture by Statistical Data Analysis

IFAC Proceedings Volumes, 1983
Abstract An algorithm for the optimization of a fermentation process was investigated using the combination of dynamic programming and a linear predictive procedure by regression analysis. It was applied to the fed-batch culture for glutamic acid production with ethanol feeding and was proved to be effective for solving the optimization problems ...
M. Kishimoto, T. Sawano, T. Yoshida, H. Taguchi   +3 more
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Controlled fed-batch by tracking the maximal culture capacity

Journal of Biotechnology, 2007
Fed-batch processes are well established in the biotech industry. The major reason to apply this technique is to avoid overflow metabolism and/or accumulation of toxic substrates. The basic idea of this approach is to control the physiological state of the culture, rather than just the typically exponential feed rate profile, by challenging a fed-batch
Henes B, Sonnleitner B
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Fed-Batch Culture Processes

2020
The cells used in cell culture processes are all derived from animals. Their genetic and epigenetic control circuits and their physiology have evolved to carry out the physiological functions of the tissue from which they were derived, not to sustain themselves and proliferate in in vitro culture.
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Generic model control of an Escherichia coli fed-batch culture

2019 23rd International Conference on System Theory, Control and Computing (ICSTCC), 2019
Generic model control (GMC), a model-based adaptive control strategy, is applied to a fed-batch high cell density cultivation of Escherichia coli, with biomass production maximization as an objective. A macroscopic model based on the overflow metabolism assumption is used, considering two metabolic pathways (respirative and respiro-fermentative), and ...
Abadli, Merouane, Dewasme, Laurent, Dumur, Didier, Tebbani, Sihem, Wouwer, Alain Vande   +4 more
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Controlling glycation of recombinant antibody in fed‐batch cell cultures

Biotechnology and Bioengineering, 2011
AbstractProtein glycation is a non‐enzymatic glycosylation that can occur to proteins in the human body, and it is implicated in the pathogenesis of multiple chronic diseases. Glycation can also occur to recombinant antibodies during cell culture, which generates structural heterogeneity in the product. In a previous study, we discovered unusually high
Inn H, Yuk, Boyan, Zhang, Yi, Yang, George, Dutina, Kimberly D, Leach, Natarajan, Vijayasankaran, Amy Y, Shen, Dana C, Andersen, Bradley R, Snedecor, John C, Joly   +9 more
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Simulation of L/A control in fed‐batch culture of baker's yeast

Biotechnology and Bioengineering, 1992
AbstractL/A controllers have extended their use from continuous to fed‐batch fermentation where the control is applied from the start of an initial batch phase. As opposed to proportional integral derivative (PID) controllers where even a startup procedure is recommended prior to fed‐batch, the L/A controller is not upset by an early connection.
P, Dantigny, M, Lakrori
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Control of endotoxin release in Escherichia coli fed-batch cultures

Bioprocess and Biosystems Engineering, 2004
High amounts of outer membrane (OM) components were released in glucose-limited fed-batch (GLFB) cultures at 37 degrees C at specific growth rates approaching 0.05 h(-1). Endotoxin analyses from a 20 degrees C GLFB culture gave similar results. An alternative fermentation technique, the temperature-limited fed-batch (TLFB) technique, reduced the ...
Marie, Svensson, Ling, Han, Gustav, Silfversparre, Lena, Häggström, Sven-Olof, Enfors   +4 more
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Fed-batch and continuous culture

1990
Abstract A variety of techniques has been developed for the submerged culture of microorganisms. Batch and fed-batch culture have been part of the art of fermentation since antiquity and are still used today for most industrial fermentations.
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Application of an On‐Line Turbidimeter for the Automation of Fed‐Batch Cultures

Biotechnology Progress, 1993
AbstractRecent advances in the technology of continuous in situ turbidity measurements in microbial cultivations are reviewed. Some characteristics of commercially available on‐line laser turbidimeters are estimated in relation to requirements of sensors for microbial cultures.
openaire   +2 more sources