Results 91 to 100 of about 14,337 (261)
Model‐Enabled Knowledge Transfer Across Cell Lines, Culture Scales and Conditions
Biotechnology and Bioengineering, EarlyView.ABSTRACT Mechanistic models are central to quantitative understanding and optimization of Chinese hamster ovary (CHO) cell culture processes, but their utility is often restricted by parameter sets calibrated for specific cell lines, scales, or operating conditions.
Luxi Yu +2 more
wiley +1 more source
The impact of PrsA over-expression on the Bacillus subtilis transcriptome during fed-batch fermentation of alpha-amylase production. [PDF]
Front Microbiol, 2022 Geissler AS +10 more
europepmc +1 more source
Biotechnology and Bioengineering, EarlyView.ABSTRACT A recombinant whole‐cell biocatalytic approach provides several advantages over isolated enzymes, such as improved enzyme stability, in vivo cofactor regeneration, and the facilitation of cascade reactions within a single cell. Whole‐cell biocatalysis has gained attention in medicinal opioid production, due to its technical and economic ...
Ali Jahanian +4 more
wiley +1 more source
A cell engineering approach to enzyme-based fed-batch fermentation. [PDF]
Microb Cell Fact, 2021 Sibley M, Ward JM.
europepmc +1 more source
Biotechnology and Bioengineering, EarlyView.ABSTRACT Chinese hamster ovary (CHO) cells are the leading host for recombinant therapeutic protein production in the biopharma industry. In this study, we investigated how feeding acidic forms of tricarboxylic acid (TCA) cycle intermediates—malic acid, succinic acid, and α‐ketoglutaric acid—affects cell culture performance and metabolism in two ...
Sarah A. Sacco +8 more
wiley +1 more source
Data-based dynamic compartment model: Modeling of E. coli fed-batch fermentation in a 600 m3 bubble column. [PDF]
J Ind Microbiol Biotechnol, 2022 Bisgaard J +5 more
europepmc +1 more source
Biotechnology and Bioengineering, EarlyView.ABSTRACT Improving cell‐specific productivity remains a central objective in biopharmaceutical manufacturing. In this study, the effect of blue light illumination on IgG1‐producing CHO DP‐12 cells was systematically evaluated across batch, fed‐batch, and perfusion cultivations in controlled 3 L bioreactor systems.
Stefanie Föller +2 more
wiley +1 more source
Dynamics Monitoring of Fed-batch E. coli Fermentation [PDF]
International Journal Bioautomation, 2017 A new method for on-line dynamics monitoring of three physiological states of fed-batch E. coli fermentation is proposed. The method is based on the acetate kinetics accepted as adaptive key parameter for recognition of current physiological state ...
Anastasiya Zlatkova, Velislava Lyubenova
doaj
L-Lactic Acid Production by Lactobacillus rhamnosus ATCC 10863
The Scientific World Journal, 2015 Lactic acid has been shown to have the most promising application in biomaterials as poly(lactic acid). L. rhamnosus ATCC 10863 that produces L-lactic acid was used to perform the fermentation and molasses was used as substrate.
Ana Lívia Chemeli Senedese +2 more
doaj +1 more source
A novel downstream process for highly pure 1,3-propanediol from an efficient fed-batch fermentation of raw glycerol by Clostridium pasteurianum. [PDF]
Eng Life Sci, 2021 Zhang C, Sharma S, Wang W, Zeng AP.
europepmc +1 more source