Results 41 to 50 of about 88,820 (301)
FEBS Letters, EarlyView.An unexpected alternative interaction site for ethyl viologen was identified in formate dehydrogenase 1 from Methylorubrum extorquens. Combined mutagenesis, kinetic analysis, and docking revealed that aromatic residues near an iron–sulfur cluster enable flavin mononucleotide‐independent electron transfer, offering a framework for engineering improved ...
Eleni G. Poloniataki, Yong Hwan Kim
wiley +1 more source
Systematic evaluation of characteristics of the membrane-based fed-batch shake flask
Microbial Cell Factories, 2017 Background The initial part of process development involves extensive screening programs to identify optimal biological systems and cultivation conditions.
P. Philip +6 more
doaj +1 more source
, 2010 In this paper, high-concentration sugars were produced from pretreated corn stover. The raw corn stover was pretreated in a process combining steam explosion and alkaline hydrogen-peroxide. The hemicellulose and lignin were removed greatly. The cellulose
Li, Qiang +5 more
core +1 more source
Fed-batch bioreactor with mortality rate
IFAC Proceedings Volumes, 2013 We address the problem of finding an optimal feedback control for feeding a fed-batchbioreactor with one species and one substrate, from a given initial condition to a given targetvalue in a minimal amount of time. Mortality rate for the biomass and nutrient recycling are taken into account in this work. The optimal synthesis (optimal feeding strategy)
Bayen, Térence +2 more
openaire +1 more source
FEBS Open Bio, EarlyView.RoboMic is an automated confocal microscopy pipeline for high‐throughput functional imaging in living cells. Demonstrated with fluorescence recovery after photobleaching (FRAP), it integrates AI‐driven nuclear segmentation, ROI selection, bleaching, and analysis.
Selçuk Yavuz +6 more
wiley +1 more source
Screening and epitope characterization of Nidogen‐2‐specific nanobodies
FEBS Open Bio, EarlyView.Camel immunization and phage display were employed to generate high‐affinity VHH nanobodies against Nidogen‐2. After library construction, biopanning, ELISA screening, sequencing, and recombinant expression, selected nanobodies were purified and characterized, leading to the preliminary exploration of a nanobody‐based sandwich ELISA for specific ...
Jianchuan Wen +9 more
wiley +1 more source
One Approach for Dynamic L-lysine Modelling of Repeated Fed-batch Fermentation [PDF]
Bioautomation, 2007 This article deals with establishment of dynamic unstructured model of variable volume fed-batch fermentation process with intensive droppings for L-lysine production.
Kalin Todorov +5 more
doaj
Electronic Journal of Biotechnology, 2014 Background: Optimization of nutrient feeding was developed to improve the growth of Bacillus subtilis in fed batch fermentation to increase the production of jiean-peptide (JAA).
Juan Zhong +5 more
doaj +1 more source
Production of nattokinase by batch and fed-batch culture of Bacillus subtilis
New Biotechnology, 2010 Nattokinase was produced by batch and fed-batch culture of Bacillus subtilis in flask and fermentor. Effect of supplementing complex media (peptone, yeast extract, or tryptone) was investigated on the production of nattokinase. In flask culture, the highest cell growth and nattokinase activity were obtained with 50 g/L of peptone supplementation.
Young-Han, Cho +9 more
openaire +2 more sources
Derivation and characterization of retinal pigment epithelium from urine‐derived iPSCs
FEBS Open Bio, EarlyView.Age‐related macular degeneration causes vision loss via RPE dysfunction and loss. Traditional iPSC therapies rely on invasive biopsies, limiting scalability. Here, we utilize urine‐derived stem cells as an accessible source to generate u‐iPSCs, successfully differentiated into pigmented RPE. This “Urine‐to‐Retina” platform provides a promising path for
Daniella Beiner +7 more
wiley +1 more source