Comprehensive clone screening and evaluation of fed-batch strategies in a microbioreactor and lab scale stirred tank bioreactor system : application on Pichia pastoris producing Rhizopus oryzae lipase [PDF]
, 2014 Background: In Pichia pastoris bioprocess engineering, classic approaches for clone selection and bioprocess optimization at small/micro scale using the promoter of the alcohol oxidase 1 gene (PAOX1), induced by methanol, present low reproducibility ...
Adelantado Vallvé, Núria +7 more
core +4 more sources
Journal of Biological Engineering, 2019 Background Fed-batch conditions are advantageous for industrial cultivations as they avoid unfavorable phenomena appearing in batch cultivations. Those are for example the formation of overflow metabolites, catabolite repression, oxygen limitation or ...
T. Keil +4 more
doaj +1 more source
Optimization of fed-batch fermentation processes using the Backtracking Search Algorithm [PDF]
, 2017 Fed-batch fermentation has gained attention in recent years due to its beneficial impact in the economy and productivity of bioprocesses. However, the complexity of these processes requires an expert system that involves swarm intelligence-based ...
Chuah, Joon Huang +3 more
core +2 more sources
Fisheries and Aquatic Sciences, 2018 As a process for commercial application, production of reducing sugar, antioxidant, and DNA protective compounds from shrimp-shell powder was investigated in a fed-batch biodegradation using Bacillus cereus EW5.
Harun Ar Rashid +2 more
doaj +1 more source
The scale-up of microbial batch and fed-batch fermentation processes [PDF]
Journal of Biotechnology, 2007 Publisher Summary The scale-up of single-celled aerobic microbial fermentation processes is complicated that can lead to unpredictable process performance. However, this is not due to the introduction of fluid dynamic generated stresses (or so-called “shear damage”), whether arising from agitator generated turbulence or bursting bubbles, rather it is
Christopher J, Hewitt, Alvin W, Nienow
openaire +2 more sources
Journal of Biological Engineering, 2020 Background Small-scale cultivation vessels, which allow fed-batch operation mode, become more and more important for fast and reliable early process development.
Timm Keil +3 more
doaj +1 more source
Strategies in fed-batch cultivation on the production performance of Lactobacillus salivarius I 24 viable cells [PDF]
, 2016 The potential use of fed-batch cultivation (FBC) for improvement of the production of Lactobacillus salivarius I 24 biomass for subsequent use as probiotics was studied using a 2-L stirredtank bioreactor. Three different constant feeding rates (0.1, 0.05,
Abd Rahim, Raha +4 more
core +1 more source
Downstream processing of high chain length polysialic acid using membrane adsorbers and clay minerals for application in tissue engineering [PDF]
, 2013 Polysialic acid (polySia) is a carbohydrate polymer of varying chain length. It is a promising scaffold material for tissue engineering. In this work, high chain length polySia was produced by an Escherichia coli K1 strain in a 10-L bioreactor in batch ...
Angata +19 more
core +2 more sources
Optimization of cyclosporin A production by Beauveria nivea in continuous fed-batch fermentation [PDF]
, 2011 To develop the effective control method for fed-batch culture of cyclosporin A production, we chose fructose, L-valine and (NH4)2HPO4 as feeding nutrients and compared their productivities in relation to different concentrations.
Dong Huijun +3 more
core +1 more source
Enhanced iturin a production in a two-compartment biofilm reactor by Bacillus velezensis ND
Frontiers in Bioengineering and Biotechnology, 2023 In this study, a two-compartment biofilm reactor was designed for iturin A production. The biofilm reactor consists of a stirred-tank fermentor containing exclusively suspended cells and a packing column where the biofilm is attached.
Zhongmin Tang +4 more
doaj +1 more source