Results 211 to 220 of about 20,856 (255)
Some of the next articles are maybe not open access.
Interaction of ferredoxin and ferredoxin-NADP reductase with thylakoids
Archives of Biochemistry and Biophysics, 1983Ferredoxin-NADP reductase accounts for about 50% of the NADPH diaphorase activity of spinach leaf homogenates. The enzyme is bound to thylakoid membranes, but can be slowly extracted by aqueous buffers. Ferredoxin-NADP reductase can be extracted from the membranes by a 1- to 2-min treatment with a low concentration of trypsin. This treatment completely
Forti G. +5 more
openaire +3 more sources
Crystals of Anabaena PCC 7119 ferredoxin-NADP+ reductase
Journal of Molecular Biology, 1991Crystals of ferredoxin-NADP+ reductase (FNR) from the cyanobacterium Anabaena PCC 7119 are grown in the presence of polyethylene glycol 6000 and beta-octyl glucoside. They belong to the hexagonal system. The cell parameters are a = b = 87.8 A, c = 92.7 A, space group P6(1) or P6(5), and a Vm of 3.0 A3/dalton for one molecule of 36,000 daltons per ...
L, Serre +4 more
openaire +2 more sources
Studies on the holoenzyme biogenesis of the spinach ferredoxin-NADP+ reductase
Archives of Biochemistry and Biophysics, 1992An expression plasmid, pPreFNR, in which the DNA sequence coding for the spinach ferredoxin-NADP+ reductase precursor was under the control of prokaryotic transcription and translation initiation signals has been constructed. The plasmid directed the synthesis in Escherichia coli of a 43-kDa immunoreactive polypeptide which could be identified with the
A. Aliverti, L. Ferretti, G. Zanetti
openaire +2 more sources
Anthracycline antibiotic reduction by spinach ferredoxin-NADP+ reductase and ferredoxin
Biochemistry, 1985Spinach NADPH:ferredoxin oxidoreductase (EC 1.6.7.1) catalyzes the NADPH-dependent reduction of the anthracyclines daunomycin, aclacinomycin A, and nogalamycin and their respective 7-deoxyanthracyclinones. Under anaerobic conditions, the endogenous rate of O2 reduction by NADPH catalyzed by ferredoxin reductase (0.12 s-1 at pH 7.4) is augmented by the ...
J, Fisher, B R, Abdella, K E, McLane
openaire +2 more sources
Molecular heterogeneity of ferredoxin-NADP+ reductase from spinach leaves
Biochimica et Biophysica Acta (BBA) - Enzymology, 1977Ferredoxin-NADP+ reductase (NADPH: ferredoxin oxidoreductase, EC 1.6.7.1) from spinach leaves has been purified according to a new procedure. The enzyme shows the presence of five molecular forms as identified by isoelectric focusing, namely a, b, c, d and e with pI values of 6.0, 5.5, 5.2, 5.0 and 4.8, respectively.
Gozzer C +5 more
openaire +2 more sources
Inhibition of pea ferredoxin–NADP(H) reductase by Zn‐ferrocyanide
European Journal of Biochemistry, 2004Ferredoxin–NADP(H) reductases (FNRs) represent a prototype of enzymes involved in numerous metabolic pathways. We found that pea FNR ferricyanide diaphorase activity was inhibited by Zn2+ (Ki 1.57 µm). Dichlorophenolindophenol diaphorase activity was also inhibited by Zn2+ (Ki 1.80 µm), but the addition of ferrocyanide was required, indicating that the
Daniela L Catalano, Dupuy +2 more
openaire +2 more sources
Complex formation by ferredoxin-NADP+ reductase with ferredoxin or NADP+
Biochimica et Biophysica Acta (BBA) - Bioenergetics, 1973Abstract Complex formation by ferredoxin-NADP + reductase (NADPH:ferredoxin oxidoreductase, EC 1.6.99.4) with ferredoxin was measured by the independent methods based on the changes of circular dichroism, fluorescence intensity and the chromatographic behavior on a Sephadex G-75 column of the two proteins after mixing.
openaire +2 more sources
Interaction of ferredoxin-NADP+ reductase from Anabaena with its substrates
Archives of Biochemistry and Biophysics, 1991The interaction of ferredoxin-NADP+ reductase from the cyanobacterium Anabaena variabilis with its substrates, NADP+ and ferredoxin, has been studied by difference absorption spectroscopy. Several structural analogs of NADP+ have been shown to form complexes the stabilities of which are strongly dependent on the ionic strength of the medium.
J, Sancho, C, Gómez-Moreno
openaire +2 more sources
In Vitro and In Vivo Interactions of Ferredoxin-NADP+ Reductases in Pseudomonas putida
The Journal of Biochemistry, 2009Ferredoxin-NADP(+) reductase (Fpr) is known to control NADP(+)/NADPH pool in proteobacteria. There is only one fpr gene present in most proteobacteria, but Pseudomonas putida has two Fprs (FprA and FprB). We elucidated the functional relationships between the two types of Fpr and their electron transport partners [ferredoxin (Fd) and flavodoxin (Fld ...
Jinki, Yeom +3 more
openaire +2 more sources
Molecular heterogeneity of ferredoxin-NADP+ reductase from spinach leaves
Biochemical and Biophysical Research Communications, 1983Highly purified ferredoxin-NADP+ reductase from spinach leaves showed at least eight different protein bands in the electrofocused gel. All of them were catalytically active and were adsorbed on a ferredoxin-Sepharose 4B affinity column. The N-terminal amino acid sequence of the main component species was analyzed by the automatic Edman degradation ...
H, Hasumi, E, Nagata, S, Nakamura
openaire +2 more sources