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Immunological studies of the binding protein for chloroplast ferredoxin-NADP+ reductase
Archives of Biochemistry and Biophysics, 1987Monospecific rabbit antibodies against the ferredoxin-NADP+ reductase binding protein of spinach thylakoids were obtained and characterized. The immunoglobulin G (IgG) fraction gave single precipitation arcs with the purified antigen or with Triton X-100 extracts of thylakoids or the reductase binding protein complex.
R L, Chan +2 more
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How is ferredoxin-NADP reductase involved in the NADP photoreduction of chloroplasts?
Photosynthesis Research, 2004NADP photoreduction of chloroplasts was discovered in 1951, and subsequent research was conducted to elucidate the enzymatic mechanisms involved in this reaction. In 1963, ferredoxin-NADP reductase (FNR; EC 1.18.1.2, ferredoxin-NADP oxidoreductase) was isolated and purified to a crystalline form.
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Purification, properties, and cellular localization of Euglena ferredoxin-NADP reductase
Biochimica et Biophysica Acta (BBA) - Bioenergetics, 1987Ferredoxin-NADP reductase from Euglena gracilis Klebs var. Bacillaris Cori purified to apparent homogeneity, yields a typical 36 kDa and an unusual 15 kDa polypeptide on sodium dodecyl sulfate-polyacrylamide gel electrophoresis, exhibits a typical flavoprotein spectrum, contains FAD, and catalyzes NADPH-dependent iodonitrotetrazolium-violet diaphorase,
A J, Spano, J A, Schiff
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[Influence of ferredoxin on ferredoxin-NADP reductase].
Planta, 2014Transhydrogenase and diaphorase activity of ferredoxin-NADP reductase are enhanced by plant ferredoxins. This stimulation is specific; ferredoxin cannot be replaced by sulfhydryl compounds such as cysteine or dithiothreitol, the apoprotein of ferredoxin or Fe(2+), Fe(3+) ions.The effect is particularly obvious with the reductase from the heterokont ...
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A lysyl residue at the NADP binding site of ferredoxin-NADP reductase
Biochimica et Biophysica Acta (BBA) - Enzymology, 1976Dansyl chloride, at low molar ratio, inactivates ferredoxin-NADP reductase (NADPH:ferredoxin oxidoreductase, EC 1.6.7.1). The complete protection afforded either by NADP or NADPH suggests a direct involvement of the active site. Experiments with [Me-14C] dansyl chloride showed that about 1.5 residues per flavin were dansylated: by differential ...
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Structure and Function of Ferredoxin-NADP Reductase Complex
1990In 1963, Shin et al. (1) first isolated ferredoxin-NADP reductase (FNR, EC. 1.18.1.2) in crystalline form from spinach and demonstrated that the crystallized enzyme reduced NADP when it was added back together with ferredoxin to illuminated chloroplasts from which “built-in” FNR had been removed.
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[40] Ferredoxin-NADP reductase from spinach
1971Publisher Summary This chapter discusses the determination of ferredoxin–NADP reductase from spinach. Ferredoxin–NADP reductase is a chloroplast flavoprotein and catalyzes the reduction of NADP with reduced ferredoxin. The crystalline enzyme shows not only ferredoxin–NADP reductase activity, but also a number of other enzymatic activities, such as ...
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Complex formation of ferredoxin-NADP reductase with ferredoxin and with NADP
Biochemical and Biophysical Research Communications, 1968M, Shin, A S, Pietro
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