Results 121 to 130 of about 1,555,668 (297)

Single‐molecule DNA flow‐stretch assays for high‐throughput DNA–protein interaction studies

FEBS Open Bio, EarlyView.
We describe an optimised single‐molecule DNA flow‐stretch assay that visualises DNA–protein interactions in real time. Linear DNA fragments are tethered to a surface and stretched by buffer flow for fluorescence imaging. Using λ and φX174 DNA, this protocol enhances reproducibility and accessibility, providing a versatile approach for studying diverse ...
Ayush Kumar Ganguli, Mohammad Nour Alsamsam, Ugnė Bagdonaitė, Van Truc Vu, Chun‐Jen Huang, Polina Kuzhir, Mindaugas Zaremba, Aurimas Kopūstas, Marijonas Tutkus   +8 more
wiley   +1 more source

GLP-1 guidelines amid an obesity epidemic: a poor fit for the Global South

Archives of Public Health
The recent World Health Organisation guideline on glucagon-like peptide-1 receptor agonists for obesity has generated intense interest in their use across low-and -middle-income countries. This correspondence examines the suitability of a pharmacotherapy-
Rechel Shrisunder, Vid Karmarkar
doaj   +1 more source

Association Between Abnormal Body Weight and Physical Fitness Levelsin Chinese Older Adults: Findings from the 5th National PhysicalFitness Surveillance in China

Xiehe Yixue Zazhi
ObjectiveBased on the data from the 5th National Physical Fitness Surveillance in China, this study aimed to explore the relationship between abnormal body weight and physical fitness levels in older adults.MethodsThe study included adults aged 60-79 ...
FAN Chaoqun, WANG Mei, FENG Qiang, WANG Jingjing, WU Dongming, SUN Zonghao, ZHU Jiarong, WU Yini   +7 more
doaj   +1 more source

Chameleon sequences reveal structural effects in proteins representing micelle‐like distribution of hydrophobicity

FEBS Open Bio, EarlyView.
Amino acids sequence of two different proteins with the same sequence (chameleon sequence—black boxes) represent in 3D structure of the proteins different secondary structures: HHHH—helical and BBB—Beta‐structural. The chains folded in water environment adopt different III‐order structures in which the chameleon fragments appear to adopt similar status
Irena Roterman, Katarzyna Stapor, Leszek Konieczny, Krzysztof Gądek, Piotr Nowakowski   +4 more
wiley   +1 more source

Similar body composition outcomes following volumetric diet and time-restricted eating in middle-aged individuals: a 12-week randomized controlled trial

Nutrition Journal
Introduction Overweight and obesity are increasing global challenges associated with severe health risks. Lifestyle factors like easy access to high-caloric foods and a decrease in physical activity contribute to weight gain. The increase in fat mass (FM)
Alicia Cloos, Stephan Geisler, Eduard Isenmann   +2 more
doaj   +1 more source

Development of human monoclonal antibodies against TARM1 by yeast display

FEBS Open Bio, EarlyView.
Human monoclonal antibodies against TARM1 are generated by yeast display‐guided selection. These antibodies bind to soluble and cell‐surface forms of TARM1. Also, these antibodies exhibit agonistic activity in the NFAT‐GFP reporter assay, indicating that TARM1 signaling can be functionally modulated by antibodies and suggesting TARM1 as a potential ...
Rikio Yabe, Mayumi Saeki, Masaaki Hashiguchi, Sayaka Ono, Kazuhisa Aoki, Hidetaka Tanno   +5 more
wiley   +1 more source

Current status and related factors of breakfast skipping among adolescents aged 12–15 years in Shandong Province, China: a cross-sectional study

BMC Public Health
Background Regular breakfast consumption is essential for adolescent health, providing critical nutrients for growth, development, and cognitive performance. However, breakfast skipping remains prevalent among adolescents globally and has been associated
Zhihao Huang, Qing Zhang, Chunyang Li, Hanhui Liu, Kunzong Tian   +4 more
doaj   +1 more source

Medical fitness for work in physicians with psychiatric disorders

European Psychiatry
Introduction Being a doctor is a profession with special medical requirements. Therefore, the assessment of medical fitness for work among physicians remains a complex decision, particularly for those with psychiatric disorders.
S. Chemingui, D. Brahim, I. Youssfi, M. Mersni, M. Methni, H. Ben Said, N. Mechergui, I. Yaich, C. Ben Said, N. Bram, N. Ladhari   +10 more
doaj   +1 more source

Establishing an assay to evaluate d‐amino acid oxidase enzyme kinetics and inhibition using WST‐8 redox dye

FEBS Open Bio, EarlyView.
This study investigated a novel WST‐8‐based assay for evaluating d‐Amino acid oxidase (DAO) inhibitors. We confirmed its effectiveness using known inhibitors and found that uremic toxins possess relatively weak inhibitory activity compared to existing drugs.
Kahoko Miyake, Yuki Enoki, Yuka Nakazawa, Kazuaki Taguchi, Kazuaki Matsumoto   +4 more
wiley   +1 more source

Analysing the significance of small conformational changes and low occupancy states in serial crystallographic data

FEBS Open Bio, EarlyView.
This protocol paper outlines methods to establish the success of a time‐resolved serial crystallographic experiment, by means of statistical analysis of timepoint data in reciprocal space and models in real space. We show how to amplify the signal from excited states to visualise structural changes in successful experiments.
Jake Hill, Yelyzaveta Pulnova, Elke De Zitter, Helen M. Ginn, Briony A. Yorke   +4 more
wiley   +1 more source